Abstract: In angiosperms, mature pollen falls on the stigma to germinate the pollen tube. Under the guidance of funiculus and micropyle signals, the pollen tube grows accurately into the embryo sac in the ovule, and then ruptures to release two sperm cells to complete double fertilization. Ensuring that only one pollen tube enters the embryo sac for complete double fertilization and stable transmission of genetic information is carefully regulated. This paper reviews recent research on the mechanisms of pollen tube guidance and polytubey block.