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杨旭,苗春妍,谢彩侠,李娟,王丰青. 水杨酸对地黄毛蕊花糖苷含量及基因表达的影响[J]. 植物科学学报,2023,41(2):204−213. DOI: 10.11913/PSJ.2095-0837.22115
引用本文: 杨旭,苗春妍,谢彩侠,李娟,王丰青. 水杨酸对地黄毛蕊花糖苷含量及基因表达的影响[J]. 植物科学学报,2023,41(2):204−213. DOI: 10.11913/PSJ.2095-0837.22115
Yang X,Miao CY,Xie CX,Li J,Wang FQ. Effects of salicylic acid on acteoside accumulation and gene expression in tuberous roots of Rehmannia glutinosa Libosch.[J]. Plant Science Journal,2023,41(2):204−213. DOI: 10.11913/PSJ.2095-0837.22115
Citation: Yang X,Miao CY,Xie CX,Li J,Wang FQ. Effects of salicylic acid on acteoside accumulation and gene expression in tuberous roots of Rehmannia glutinosa Libosch.[J]. Plant Science Journal,2023,41(2):204−213. DOI: 10.11913/PSJ.2095-0837.22115

水杨酸对地黄毛蕊花糖苷含量及基因表达的影响

Effects of salicylic acid on acteoside accumulation and gene expression in tuberous roots of Rehmannia glutinosa Libosch.

  • 摘要: 为了分析叶片表面喷施水杨酸(SA)对毛蕊花糖苷含量的影响及其分子调控特征,以地黄(Rehmannia glutinosa Libosch.)栽培品种‘温85-5’为材料,用100 μmol/L的SA喷施生长180 d的植株,在处理0、1、3和6 h后收集叶片和块根,测定毛蕊花糖苷的含量,并对块根进行转录组测序分析。结果显示,SA处理后,地黄叶片中的毛蕊花糖苷提高了11.2% ~ 19.3%,块根中的毛蕊花糖苷含量提高了0.9 ~ 1.4倍。转录组分析结果表明,SA处理3 h后的差异表达基因最多,且下调表达的基因多于上调表达。苯乙醇苷生物合成通路在差异表达基因中得到显著富集,毛蕊花糖苷合成途径的催化酶基因如ALDHUGTPPO等在SA处理后的地黄块根中上调表达。此外,本文还鉴定出多个在SA处理后差异表达的AP2-EREBP、WRKY和MYB等转录因子基因。

     

    Abstract: The Rehmannia glutinosa ‘Wen 85-5’ cultivar was used to analyze the effects of spraying salicylic acid (SA) on the leaf surface on acteoside content and molecular regulation characteristics. The plants were grown for 180 days, then sprayed with SA (100 μmol/L). The leaves and tuberous roots of R. glutinosa were collected at 0, 1, 3, and 6 h after treatment to determine the content of acteoside. Transcriptome sequencing of the tuberous roots at different times after SA treatment was also performed. Results showed that, compared with the controls, acteoside content in the leaves and tuberous roots increased by 11.2% - 19.3%, and 0.9 - 1.4 times, respectively, after SA treatment. Transcriptome analysis showed that most differentially expressed genes (DEGs) were obtained 3 h after SA treatment, with more down-regulated genes than up-regulated genes. Most DEGs were significantly enriched in the phenylpropanoid biosynthesis pathway, while several catalytic enzyme genes of the acteoside synthesis pathway, such as ALDH, UGT, and PPO, were up-regulated in the tuberous roots. Many AP2-EREBP, WRKY, and MYB transcription factor genes were differentially expressed after SA treatment. This study provides theoretical support for the use of elicitors to treat R. glutinosa plants in the field to increase acteoside content.

     

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