Abstract: GDP-mannose pyrophosphorylase (GMP) is a key enzyme in the plant polysaccharide synthesis pathway. In this paper, members of the GMP gene family in Gossypium hirsutum L. were identified, their expression patterns in different tissues and under different abiotic stresses were analyzed, and evolutionary analysis between species and GMP gene families of other model plants was carried out. Results showed that G. hirsutum L. and G. barbadense L. both contained 38 GMP family members, G. raimondii Ulbrich contained 19 members, and G. arboreum L. contained 18 members. Based on phylogenetic analysis, 141 GMPs from different plants were divided into six subgroups. GhGMP members of the same subfamily exhibited similar gene structures and conserved motifs, and the promoter region contained many cis-acting element related to hormone response, plant growth and development, and stress response. Transcriptomic analysis showed that the expression of GhGMP genes was highest in the leaves, and some genes were significantly up-regulated after abiotic stress.