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许晨, 李青云, 万俊男, 侯雨君, 周慧敏, 朱振飞, 辛海平, 李吉涛. 葡萄Golden2-like转录因子家族的鉴定及其表达分析[J]. 植物科学学报, 2022, 40(2): 205-215. DOI: 10.11913/PSJ.2095-0837.2022.20205
引用本文: 许晨, 李青云, 万俊男, 侯雨君, 周慧敏, 朱振飞, 辛海平, 李吉涛. 葡萄Golden2-like转录因子家族的鉴定及其表达分析[J]. 植物科学学报, 2022, 40(2): 205-215. DOI: 10.11913/PSJ.2095-0837.2022.20205
Xu Chen, Li Qinq-Yun, Wan Jun-Nan, Hou Yu-Jun, Zhou Hui-Min, Zhu Zhen-Fei, Xin Hai-Ping, Li Ji-Tao. Identification and expression analysis of the Golden2-like transcription factor family of Vitis vinifera L.[J]. Plant Science Journal, 2022, 40(2): 205-215. DOI: 10.11913/PSJ.2095-0837.2022.20205
Citation: Xu Chen, Li Qinq-Yun, Wan Jun-Nan, Hou Yu-Jun, Zhou Hui-Min, Zhu Zhen-Fei, Xin Hai-Ping, Li Ji-Tao. Identification and expression analysis of the Golden2-like transcription factor family of Vitis vinifera L.[J]. Plant Science Journal, 2022, 40(2): 205-215. DOI: 10.11913/PSJ.2095-0837.2022.20205

葡萄Golden2-like转录因子家族的鉴定及其表达分析

Identification and expression analysis of the Golden2-like transcription factor family of Vitis vinifera L.

  • 摘要: 本研究对葡萄(Vitis vinifera L.)的Golden2-like (GLK)转录因子家族进行了全基因组鉴定和表达模式分析,并利用品种‘玫瑰香’(V.vinifera cv.Muscat Hamburg)进一步验证其在低温胁迫下的响应。结果显示,葡萄Golden2-like家族共46个成员,分为5个亚族,同一亚族的保守结构域相似。46个VvGLK分别定位于细胞核、叶绿体、细胞质和过氧化物酶体中,其启动子区域含多种逆境应答顺式作用元件。基因芯片分析结果表明,22个Golden2-like基因在果实发育过程中变化显著。同时,有15、15和9个基因分别响应盐、干旱和低温胁迫。qRT-PCR分析发现26个基因参与低温应答。VvGLK41在所有胁迫处理中均下调表达。

     

    Abstract: In this study, the Golden2-like transcription factor (TF) gene family was identified and analyzed in Vitis vinifera L. We further verified the expression patterns of Golden2-like genes under low temperature in V. vinifera ‘Muscat Hamburg’ seedlings. Results showed that 46 Golden2-like genes were identified in V. vinifera and classified into five subgroups. The conserved domain was similar within the same subgroup. Forty-six VvGLKs were located in the nucleus, chloroplast, cytoplasm, and peroxisome, and their promoter regions contained a variety of cis-elements related to stress response. Gene chip data showed that 22 VvGLKs changed significantly during fruit development and 15, 15, and 9 VvGLKs were expressed after salt, drought, and low-temperature stress, respectively. qRT-PCR identified 26 VvGLKs involved in cold-stress response. Notably, VvGLK41 was down-regulated after salt, drought, and low-temperature treatment. Thus, this study provides clues for fruit development and stress response in grape.

     

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