淫羊藿花蕾cDNA文库的构建与鉴定

Construction and Identification of a cDNA Library for the Flower buds of Epimedium brevicornu Maxim

摘要: 基于smart技术构建了淫羊藿花蕾cDNA文库并检测了其质量。结果表明,该文库重组率为95%,平均插入片段大小为1095 bp,文库滴度为2×10⁶pfu/mL,是一个高质量的淫羊藿花蕾cDNA文库。此文库的建立将有助于克隆与次生代谢相关的基因,特别是淫羊藿黄酮特异合成代谢的基因,其次是克隆与花发育相关的基因。

Abstract: A cDNA library for flower buds of Epimedium brevicornu Maxim was constructed which based on SMART technology.Our results showed that the titer of unamplifed library from flower buds was 2×10⁶ pfu/mL.The percentage of recombinants was 95% and the average length of the insert cDNA fragments was 1095 bp.It suggested that one high quality cDNA library for flower buds of E.brevicornu were successfully constructed.Construction of cDNA library will help to clone the relevant genes associated with secondary metabolism,first,the special genes in the course of flavonoid biosynthesis;second,the genes related with development of flower buds in E.brevicornu Maxim.