莲藕品种DNA指纹图谱的绘制

详细信息

Construction of the DNA Fingerprinting in Nelumbo

1.
Key Laboratory of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072, China
2.
Lotus Center, Wuhan University, Wuhan 430072, China

摘要: 采用RAPD技术对14个莲藕品种进行遗传多态性分析,用5个Operon引物和80个SBS的RAPD引物进行筛选,从中选出来自SBS的RAPD-C13和RAPD-D15扩增出的8条多态性条带,绘制了14个品种的DNA指纹图谱,在该图谱中每个品种均有各自特异的DNA指纹。
- DNA指纹 /
- 遗传多态性 /
- 莲藕 /
- RAPD
Abstract: The genetic polymorphism was analyzed by RAPD(random amplified polymorphic DNA)in 14 lotus accessions.Eight stable and repeatable RAPD bands amplified with two primers,RAPD-C13 and RAPD-D15 coming from five Operon’s primers and eighty SBS’s primers,were selected for the construction of DNA fingerprinting,in which each lotus had its unique fingerprinting pattern.
- DNA fingerprinting /
- Genetic polymorphism /
- Nelumbo /
- RAPD