Wild Rice Gene Expression in Response to Brown Planthopper (Nilaparvata lugens Stål) Feeding and Whitebacked Planthopper (Sogatella furcifera Horvath) Feeding
-
摘要: 褐飞虱和白背飞虱是水稻的刺吸式害虫。为了探讨水稻对虫害反应的分子机理,采用cDNA-AFLP(互补DNA扩增片段长度多态性)技术,研究了药用野生稻受这两种飞虱取食后基因表达的变化。通过调查约7%的虫应答后的药用野生稻转录本组,共分离了258个差异片段,测序112个;44个片段对应于已知或假定功能基因,其中35个基因的表达水平在白背飞虱为害后发生改变,24个基因在褐飞虱为害后改变。下调的基因比上调的基因多。有14个基因受这两种飞虱共同调节(2个上调,12个下调)。结果表明,药用野生稻对两种飞虱为害作出的反应有所不同。Abstract: Brown planthopper (Nilaparvata lugens (Stål),BPH) and whitebacked planthopper (Sogatella furcifera Horvath,WBPH) are the phloem-sucking herbivores of rice.We employed cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique to characterize the mole-(cular) responses of wild rice Oryza officinalis Wall ex Watt to BPH and WBPH feeding.After investigating about 7% of the insects-responsive transcriptome,we isolated 258 differential transcript-derived fragments (TDFs) and sequenced 112,of which 44 presented similarities to known or putative functional proteins.Among these 44 TDFs,35 TDFs were altered by WBPH-feeding while 24 were by BPH-feeding.There were more down-regulated TDFs than the up-regulated ones found after fed by BPH and WBPH.Only 2 TDFs were co-induced but 12 were co-suppressed by two planthoppers feeding.The results revealed that O.officinalis responded differentially to these two phloem herbivores.
-
计量
- 文章访问数: 3495
- HTML全文浏览量: 0
- PDF下载量: 1773
下载:
