Genetics Analysis and Identification of Hybrid Rice HL-Type (Honglian-2) and Their Backbone Parental with RAPD Markers

Atotal of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD(random amplified polymorphic DNA) technique on the genome DNAs of 14 rice materials which included HLtype hybrid rice (Honglian-2) and their parental(T-07A,T-07B,YD6-05),six other backbone restore lines of CMS-Honglian type,Shanyou 63 and their parental.Eighteen primers produced 173 polymorphic fragments.The results from cluster analysis showed:There was little genetic diversity between sterile lines and maintainer lines because of their similar nuclear background.The genome-type of hybrid rice was inclined to restore lines.There was large genetic variation between restorer lines and sterile lines,but the genetic distance was small among restorer lines.Using this RAPDmarkers,the hybrid rice combinations (sterile line,maintainer line,resto rer line and hybrid rice(F₁)) could be effectively identified.