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SU Ping, ZHAO Jin-Mei, ZHAO Kai-Hong, ZHOU Ming. Aggregation Research of Allophycocyanin[J]. Plant Science Journal, 2008, 26(6): 573-580.
Citation: SU Ping, ZHAO Jin-Mei, ZHAO Kai-Hong, ZHOU Ming. Aggregation Research of Allophycocyanin[J]. Plant Science Journal, 2008, 26(6): 573-580.
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Aggregation Research of Allophycocyanin

  • 1.

    Hubei Normal University, Huangshi, Hubei 435002, China

  • 2.

    College of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

  • 3.

    State Key Laboratory for Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

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  • Received Date: March 23, 2008
  • Revised Date: May 28, 2008
  • Published Date: December 29, 2008
    Graphical Abstract
    • Abstract
  • In order to study the biosynthesized α-APC and β-APC,chromoprotein PCB-ApcA and PCB-ApcB was obtained by in vivo reconstitution in Escherichia coli,and was shown through the absorption spectra and the fluorescence spectra.A plasmid containing apcA and apcB was constituted by inserting this two genes in the duet vector with two multiple clone sites separately.Chromoprotein PCB-ApcA and PCB-ApcB could be biosynthesized in vivo simultaneously in E.coli.Through in vivo reconstitution of multiple combinations,(αAPCβAPC)3 could be detected in the cell and αAPCβAPC in the supernatant.
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