Recent Advances in Detection Techniques of Binding Plant Calcium-binding Proteins to Calcium Ions
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摘要: Ca2+在植物生长发育和环境适应过程中发挥着中心调控作用, 钙信号是植物生长发育和逆境响应的主要调控因子, 钙结合蛋白是植物钙信号传导途径的最重要组分之一, 然而植物钙结合蛋白在体内和体外与Ca2+结合的技术体系还有待完善和发展。为了系统总结植物钙结合蛋白的鉴定方法与技术, 本文从定性结合、定量结合和结合方式等角度, 综述了植物钙结合蛋白在体内和体外条件下与Ca2+结合的原理、方法、特点和应用前景, 详细阐述了近年来的主要检测方法, 并对其今后的发展趋势作了展望。本文将为植物钙结合蛋白的分离、功能鉴定和作用机制的研究提供技术支撑。Abstract: Calcium ions play a central role in regulating plant growth, development and adaptation to environmental stresses. Calcium signals are one of the main regulators of plant growth, development and stress response, and calcium-binding proteins have also been identified as one of the most important components of calcium signal transduction pathways in plants. However, the assay techniques of calcium-binding proteins binding to calcium ions have not yet been evaluated in vitro and in vivo. Here, from the viewpoints of qualitative binding, quantitative binding and binding patterns, recent progresses in the principles, techniques, characteristics and applications of in vitro and in vivo calcium ion binding assays of calcium-binding proteins are reviewed, and the main detection methods and development trends in this field in recent years are also discussed in detail. Meanwhile,this review provides a new platform for isolation, functional identification and regulation mechanism studies of plant calcium-binding proteins in the future.
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Keywords:
- Calcium signal /
- Calcium-binding protein /
- Detection methods /
- Principle /
- Application
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