Phospholipase D and Lipoxygenase of Young Loquat Fruits in Response to Low Temperature Stress
-
摘要: 以3年生枇杷品种 ‘早钟6号’(Eriobotrya japonica ‘Zaozhong No.6’)容器嫁接苗为试材,于0℃、-1℃、-3℃人工气候室内进行低温胁迫处理,探讨枇杷幼果细胞膜磷脂及相关酶对低温胁迫的响应机制。结果显示,在不同温度胁迫过程中,枇杷幼果磷脂酶D (PLD, EC 3.1.4.4)和脂氧合酶(LOX, EC 1.13.11.12)活性均呈上升趋势;质膜磷脂酰胆碱(PC)和磷脂酰肌醇(PI)含量因逐渐被降解而呈下降趋势,磷脂酸(PA)含量出现积累、增加,而膜结合Ca2+含量有不同程度的降低。随处理时间的延长和处理温度的降低,枇杷幼果细胞PLD和LOX活性增幅加大,从而加速了膜PC和PI的降解和PA的积累。低温胁迫过程中幼果细胞膜PC含量的降幅大于PI,膜结合Ca2+含量的变化与PLD和LOX活性变化呈负相关。低温胁迫下枇杷幼果细胞膜结合Ca2+含量的减少诱导了膜脂降解酶PLD和LOX活性的提高,并导致膜结构稳定性下降,加剧了低温胁迫对膜脂的降解和脂质过氧化伤害,其中尤以-3℃胁迫处理4~6 h对幼果细胞质膜的伤害最严重。表明低温胁迫下Ca2+·CaM信使系统可能参与枇杷幼果细胞膜PLD和LOX活性的调控。Abstract: Container grafted seedlings of three-year-old loquat (Eriobotrya japonica ‘Zaozhong No.6’) were treated at 0℃, -1℃ and -3℃ in phytotron to determine the mechanism of responses of membrane phospholipid and related enzymes in young loquat fruits under low temperature stress. Results showed that the activities of phospholipase D (PLD, EC 3.1.4.4) and lipoxygenase (LOX, EC 1.13.11.12) increased in young loquat fruits under different low temperature stress. The contents of phosphatidylcholine (PC) and phosphatidylinositol (PI) decreased due to gradual degradation. Phosphatidic acid (PA) accumulations increased, and the membrane-associated Ca2+ contents were reduced to different degrees. With increasing stress time and decreasing stress temperature, the activities of PLD and LOX increased, resulting in accelerated PC, PI degradations and PA accumulation. The decrease in PC content was greater than that of PI content in the membranes of young loquat fruits under low temperature stress. There was a negative correlation between PLD, LOX activities and membrane-associated Ca2+ contents. The increase in PLD and LOX activities was caused by the decrease in membrane-associated Ca2+ contents. Meanwhile, the decrease in membrane-associated Ca2+ contents resulted in stable decreases in membrane structure, which aggravated membrane damage due to lipid degradation and peroxidation. The damage to the cell membranes of young fruits was most serious under low temperature stress at -3℃ for 4-6 h. The signal system of Ca2+·CaM may be involved in regulating PLD and LOX activities of young loquat fruits under low temperature stress.
-
-
[1] 谢钟琛, 李健. ‘早钟6号’枇杷幼果冻害温度界定及其栽培适宜区区划[J]. 福建果树, 2006(1): 7-11. [2] 吴锦程, 陈建琴, 梁杰, 杨伟搏, 吴晶晶, 陈丽钦, 刘美琼, 陈丽平. 外源一氧化氮对低温胁迫下枇杷叶片AsA-GSH循环的影响[J]. 应用生态学报, 2009, 20(6): 1395-1400. [3] 陈少裕. 膜脂过氧化对植物细胞的伤害[J]. 植物生理学通讯, 1991, 27(2): 84-90. [4] Croft KPC, Juttner F, Slusarenko AJ. Volatile pro-ducts of the lipoxygenase pathway evolved from Phaseolus vulgaris(L.) leaves inoculated with Pseudomonas syringae pv.phaseolicola[J].Plant Physiol, 1993, 101(1): 13-24.
[5] Flores A, Grau A, Laurich F, Dörfing K. Effect of new terpenoid analogues of abscisic acid on chil-ling and freezing resistance[J].J Plant Physiol, 1988, 132(3): 362-369.
[6] Leshem YY. Membrane phospholipids catabolism and Ca2+ activity in control of senescence[J].Physiol Plant, 1987, 69(3): 551-559.
[7] 赵鹏, 王道龙.磷脂酶D对拟南芥抗冻性的影响[J]. 中国农学通报, 2011, 27(6): 107-111. [8] Pinhero RG, Paliyath G, Yada RY, Murr DP. Mo-dulation of phospholipase D and lipoxygenase activities during chilling. Relation to chilling tole-rance of maize seedlings[J].Plant Physiol Biochem, 1998, 36(3): 213-224.
[9] Pinhero RG, Almquist KC, Novotna Z, Paliyath G. Developmental regulation of phospholipase D in tomato fruits[J].Plant Physiol Biochem, 2003, 41(3): 223-240.
[10] Paliyath G, Pinhero RG, Yada RY, Murr DP. Effect of processing conditions on phospholipase D activity of corn kernel subcellular fractions[J].J Agric Food Chem, 1999, 47(7): 2579-2588.
[11] 李靖, 马长乐. 植物脂氧合酶研究进展[J]. 生物学杂志, 2007, 24(6): 5-8. [12] Grechkin A. Recent developments in biochemistry of the plant lipoxygenase pathway[J].Progr Lipid Res, 1998, 37(5): 317-352.
[13] Wang XM. Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses[J].Plant Physiol, 2005, 139(2): 566-573.
[14] Bargmann BOR, Munnik T. The role of phospholipase D in plant stress responses[J].Curr Opin Plant Biol, 2006, 9(5): 515-522.
[15] Bargmann BOR,Laxalt AM, Riet BT, Testerink C, Merquiol E, Mosblech A, Leon-reyes A, Pie-terse CMJ, Haring MA, Heilmann I, Bartels D, Munnik T.Reassessing the role of phospholipase D in the Arabidopsis wounding response[J].Plant Cell Environ, 2009, 32(7): 837-850.
[16] Khatoon H, Talat S, Younus H. Phospholipase D from Allium sativum bulbs: a highly active and thermal stable enzyme[J].Int J Biol Macromol, 2008, 42(4): 380-385.
[17] 石延霞, 于洋, 傅俊范, 李宝聚. 病原菌诱导后黄瓜叶片中脂氧合酶活性与茉莉酸积累的关系[J]. 植物保护学报, 2008, 35(6): 486-490. [18] Yapa PAJ, Kawasaki T, Matsumoto H. Changes of some membrane-associated enzyme activities and degradation of membrane phospholipids in cucumber roots due to Ca2+ starvation[J].Plant Cell Physiol, 1986, 27(2): 223-232.
[19] Mao LC, Wang GZ, Zhu CG, Pang HQ. Involvement of phospholipase D and lipoxygenase in response to chilling stress in postharvest cucumber fruits[J].Plant Sci, 2007, 172(2): 400-405.
[20] 杨文龙, 杨玉焕, 阎秀峰, 王洋. HPLC法测定树木叶片中磷脂含量[J]. 东北林业大学学报, 2008, 36(9): 61-62. [21] Welti R, Li WQ, Li MY, Sang YM, Biesiada H, Zhou HE, Rajashekar CB, Williams TD, Wang XM. Profiling membrane lipids in plant stress responses: Role of phospholipase D in freezing-induced lipid changes in Arabidopsis[J].J Biol Chem, 2002, 277(35): 31994-32002.
[22] Uemura M, Joseph RA, Steponkus PL. Cold acclimation of Arabidopsis thaliana(Effect on plasma membrane lipid composition and freeze-induced lesions)[J].Plant Physiol, 1995, 109(1): 15-30.
[23] Paliyath G, Thompson JE. Calcium-and calmodulin-regulated breakdown of phospholipid by microsomal membranes from bean cotyledons[J].Plant Physiol, 1987, 83(1): 63-68.
[24] Paliyath G,Droillard MJ. The mechanisms of membrane deterioration and disassembly during senescence[J].Plant Physiol Biochem, 1992, 30(6): 789-812.
[25] Wang, XM. Multiple forms of phospholipase D in plant: the gene family, catalytic and regulatory properties, and cellular functions[J].Progr Lipid Res, 2000, 39(2): 109-149.
[26] Smith IK. Role of calcium in serine transport into tobacco cells[J].Plant Physiol, 1978, 62(6): 941-948.
[27] 刘祖褀, 张石城. 植物抗性生理学[M]. 北京: 中国农业出版社, 1994: 8-9, 21. [28] 简令成. 植物抗寒机理研究的新进展[J]. 植物学通报, 1992, 9(3): 17-22. [29] 王建华, 刘鸿先, 徐同. 超氧化物歧化酶(SOD)在植物逆境和衰老生理中的作用[J]. 植物生理学通讯, 1989, 11(1): 1-7. [30] 罗广华, 王爱国, 邵从本, 郭俊彦. 高浓度氧对种子萌发和幼苗生长的伤害[J]. 植物生理学报, 1987, 13(2): 161-167. [31] Willemot C. Rapid degradation of polar lipids in frost damaged winter wheat crown and root tissue[J].Phytochem, 1983, 22(4): 861-863.
[32] 吴敏, 陈昆松, 张上隆. 桃果实采后成熟衰老过程中脂氧合酶活性的变化[J]. 园艺学报, 1999, 26(4): 227-231. [33] 陈昆松, 张上隆. 脂氧合酶与果实的成熟衰老——文献综述[J]. 园艺学报, 1998, 25(4): 338-344. [34] Gardner HW. Recent investigation into the lipoxygenase pathway of plants [J].Biochim Biophys Acta, 1991, 1084 (3): 221-239.
[35] 周瑞莲, 赵哈林. 高寒山区牧草根质膜和脂肪酸组分对冷冻低温的适应反应[J]. 植物生态学报, 2001, 25(1): 115-118. [36] Deng LY. Study on relationship between membrane lips fatty acid and winter hardy in grape[J].Acta Phytophysiol Sin, 1982, 8(3): 273-281.
[37] Murelli C, Rizza F, Albini FM, Dulio A, Terzi V, Cattivelli L. Metabolic changes associated with cold-acclimation in contrasting cultivars of barley[J].Physiol Plant, 1995, 94(1): 87-93.
[38] 雷江丽, 杜永臣, 朱德蔚, 王鸣, 王孝宣, 李树德. 低温胁迫下不同耐冷性番茄品种幼叶细胞Ca2+分布变化的差异[J]. 园艺学报, 2000, 27(4): 269-275. [39] 高洪波, 陈贵林. 钙调素拮抗剂与Ca2+对茄子幼苗抗冷性的影响[J]. 园艺学报, 2002, 29(3): 243-246. [40] 于锡宏, 蒋欣梅, 刁艳, 王丽丽, 刘在民, 于广建. 脱落酸、水杨酸和氯化钙对番茄幼苗抗冷性的影响[J]. 东北农业大学学报, 2010, 41(5): 42-45. [41] 刘峰, 张军, 张文吉. 氧化钙对水稻幼苗的生理作用研究[J]. 植物学通报, 2001, 18(4): 490-495. [42] 苗永美, 王万洋, 杨海林, 居文军, 戈应祥, 高青海, 贾双双. 外源Ca2+、SA和ABA缓解甜瓜低温胁迫伤害的生理作用[J]. 南京农业大学学报, 2013, 36(4): 25-29. [43] Sang YM, Cui DC, Wang XM. Phospholipase D and phosphatidic acid-mediated generation of superoxide in Arabidopsis[J].Plant Physiol, 2001, 126(4): 1449-1458.
[44] 王慧, 彭建平, 方树民, 郑国栋, 邱春锦, 黄强, 陈玉森, 郑国华. 枇杷幼果中定殖的冰核细菌与冻害关系[J]. 植物保护, 2008, 34(2): 43-46.
计量
- 文章访问数: 1325
- HTML全文浏览量: 0
- PDF下载量: 1083
下载:
