Analysis on Volatile Components of Caryophylli Flos from Different Habitats
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摘要: 采用顶空固相微萃取-气相色谱-质谱联用技术对产自国内外4个地区的丁香(Eugenia caryophyllata Thunb.)挥发性成分进行了检测,并以峰面积归一化法计算了各组分的相对含量。结果显示,从4个产地13批次丁香中共鉴定出挥发性成分72种(匹配度均高于75),其中,主要成分含量排名前两位的均为丁香酚、(-)-α-芹子烯,排名第三位的挥发性成分分别为:顺式-α-没药烯(马达加斯加塔马塔夫,平均为3.75%)、罗勒烯(中国广东,平均为4.21%)、亚麻三烯(中国广西,平均为3.74%)、丁香烯(印度尼西亚爪哇岛,平均为3.60%)。表明同一产地的丁香挥发性成分具有一定的相似性,不同产地丁香挥发性成分也有一定的差异,这对丁香产地的鉴别具有重要意义。主成分分析和聚类分析的结果既可以将4个产地的丁香很好地区分开来,又能反映出它们之间的亲缘关系。本研究采用HS-SPME-GC-MS方法检测不同产地丁香挥发性成分并结合主成分分析(PCA)及聚类分析法,能有效区分4个产地的丁香,该方法可作为丁香产地的鉴别方法,也为进一步比较不同产地丁香挥发性成分的差异及质量控制提供了新思路。
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关键词:
- 丁香 /
- 顶空固相微萃取-气质联用 /
- 挥发性成分 /
- 主成分分析 /
- 聚类分析
Abstract: We analyzed the volatile chemical components of Caryophylli Flos from different habitats using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and roughly quantified by peak area normalization. In total, 72 peaks were identified from 13 batches of Caryophylli Flos. Among the volatile chemical components, Eugenol and (-)-α-Selinene accounted for the first two main components, with third-ranked components of the four habitats being cis-α-bisabolene (Madagascar Tamatave, average 3.75%), Ocimene (Guangdong Province, China, average 4.21%), Santolinatriene (Guangxi Province, China, average 3.74%), Humulene (Indonesia Java, average 3.60%). These results suggest that the volatile components of Caryophylli Flos differed in different regions, but showed similarities within the same region. Using principal component analysis (PCA) and cluster analysis, results showed that the four habitat samples of Caryophylli Flos were not only easily distinguished, but also reflected a genetic relationship between them. HS-SPME-GC-MS combined with stoichiometry (PCA and cluster analysis) was able to distinguish the four origins of Caryophylli Flos. This study provided a new approach for the comparison and quality assessment of Caryophylli Flos from different habitats. -
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[1] 国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2010: 4. [2] Wong RW, Hägg U, Samaranayake L, Yuen MK, Seneviratne CJ, Kao R. Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm[J]. Int J Oral Maxillofac Surg, 2010, 39(6): 599-605.
[3] 朱金段,袁德俊,林新颖.丁香的药理研究现状及临床应用[J].中国药物经济学,2013,21(1): 32-35. [4] 张军锋,张树军.丁香属植物的化学成分及其药理作用的研究进展[J].海南大学学报:自然科学版,2007,25(2): 200-205. [5] 宋光西,马玲云,魏锋,马双成.丁香属植物的化学成分分类及药理作用研究进展[J]. 亚太传统医药,2011,10(5): 179-181. [6] 俞懿强,邹德荣.丁香酚的药理作用及在口腔医学应用的研究进展[J].临床口腔医学杂志,2009,25(3): 187-189. [7] 李博,朱俊访.丁香叶中所含有效成分的种类及药理作用研究进展[J].黑龙江医药,2009,22(4): 510-511. [8] 彭宅彪,张琼光,代虹健,丁英平.丁香酚的药理学研究进展[J].时珍国医国药,2006,17(10): 2079-2081. [9] 喻莉萍,张辉,彭爱姣. 药学类科技期刊的主成分分析及综合评价[J]. 科技情报开发与经济,2014,24(1): 121-123. [10] 张文斌,平艳玲,史艳萍. SPSS软件在药学研究领域中的应用[J]. 解放军药学学报,2003,19(1): 76-78. [11] Srivastava AK, Srivastava SK, Syamsundar KV. Bud and leaf essential oil composition of Syzygium aromaticum from India and Madagascar[J].Flavour Frag J, 2005, 20(1): 51-53.
[12] 邱琴,崔兆杰,赵怡.丁香挥发油化学成分的GC-MS分析[J].中药材,2003, 26(1): 25-26. [13] 邱电,张魁华,方炳虎.丁香酚的药理作用[J].动物医学进展,2007,28(8): 101-103. [14] 吕世明,陈杖榴,陈建新,邱电,李春梅.丁香酚体外抑菌作用研究[J].食品科学,2008,29(9): 122-124. [15] 张杰,王春梅,程罗根,陈浩,石志琦.丁香酚对灰葡萄孢的抑制作用研究[J].农药学学报,2008,10(1): 68-74. [16] Al-Sharif I, Remmal A, Aboussekhra A. Eugenol triggers apoptosis in breast cancer cells through E2F1/survivin down-regulation[J] BMC Cancer, 2013, 13(13): 600-610.
[17] Estevão-Silva CF, Kummer R, Fachini-Queiroz FC, Grespan R, Nogueira de Melo GA, Baroni S, Cuman RK, Bersani-Amado CA. Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by fMLP, LTB4, and carrageenan[J] J Nat Med, 2014, 68(3): 567-575.
[18] Liu Z, Niu W, Yang X, Wang Y. Effects of combined acupuncture and eugenol on learning-me-mory ability and antioxidation system of hippocampus in Alzheimer disease rats via olfactory system stimulation[J] J Tradit Chin Med, 2013, 33(3): 399-402.
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