葡萄<em>COR27</em>基因的克隆与抗寒功能研究

范高韬; 孙小明; 任小蝶; 李绍华; 辛海平; 王万军

doi:10.11913/PSJ.2095-0837.2015.30346

葡萄COR27基因的克隆与抗寒功能研究

1. 西南交通大学生命科学与工程学院, 成都 610031;
2. 中国科学院植物种质创新与特色农业重点实验室, 中国科学院武汉植物园, 武汉 430074;
3. 中国地质大学环境学院, 武汉 430074

详细信息

作者简介:
范高韬(1989-),男,硕士,研究方向为葡萄抗逆机理(E-mail:fangaotao@126.com)。

通讯作者:
辛海平,E-mail:xinhaiping215@hotmail.com

王万军,E-mail:wanjunwang@home.swjtu.edu.cn

中图分类号: Q943.2;S663.1
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出版历程
- 收稿日期: 2015-01-19
- 网络出版日期: 2022-10-31
- 发布日期: 2015-06-27

Cloning and Functional Analysis of COR27 from Vitis vinifera

1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;
2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
3. School of Environmental Studies, China University of Geosciences, Wuhan 430074, China

摘要

摘要: 冬季低温是制约我国葡萄和葡萄酒产业发展的主要因素之一, 揭示葡萄在冷胁迫下的信号转导通路、挖掘抗寒相关基因并解析其功能, 对高耐寒品种的培育具有重要的理论和应用价值。本研究在欧亚种‘玫瑰香’葡萄(Vitis vinifera L. ‘Muscat Hamburg’)冷胁迫相关转录组分析的基础上, 鉴定了一个抗寒候选基因, 通过同源性分析将其命名为VvCOR27。VvCOR27基因的cDNA序列(1082 bp)中, 其开放阅读框(ORF)为909 bp, 编码302个氨基酸。同源性分析显示, 13个物种的COR27蛋白均具有3个特有的保守结构域。定量RT-PCR分析表明, VvCOR27在4℃低温处理24 h后大量表达。基于基因组序列的启动子基序分析表明, VvCOR27启动子区均只含有1个EE、EEL、G-box、ABREL元件, 其数量少于AtCOR27, 这可能是VvCOR27响应冷胁迫较AtCOR27滞后的原因。对3个超表达VvCOR27转基因拟南芥株系的抗寒性鉴定表明, VvCOR27参与了植株对冷胁迫的应答并作为正调控因子增强了植株对冷胁迫的耐受能力。
- 葡萄 /
- COR27基因 /
- 冷处理 /
- 抗寒性 /
- 转基因拟南芥
Abstract: Low winter temperature is one of the main factors that affect the development of grapes and the wine industry in China. Understanding the signal transduction pathway during cold stress will help in the breeding of high cold-resistance cultivars. Based on our previous transcriptome analysis, a gene that showed increased expression pattern in Vitis vinifera L. ‘Muscat Hamburg’ during cold treatment was identified and named VvCOR27 according to homological analysis. The whole length of VvCOR27 cDNA was 1082 bp, which contained a 909 bp open reading frame (ORF) and encoded 302 amino acids. Homological analysis of COR27s from thirteen species showed that they contained three COR27-specific conservative domains. Real time RT-PCR indicated that the transcript abundance of VvCOR27 was highly increased at 24 h after cold treatment. Four motifs, including EE, EEL, G-box and ABREL, were found in the promoter regions (from published Vitis vinifera genome sequences) of VvCOR27 but at less quantity than that in the promoter regions of AtCOR27. This may be why AtCOR27 showed more timely responses to cold treatment than did VvCOR27. Phenotypic analysis of three overexpression lines under cold treatment indicated that VvCOR27 was involved in responses to cold stress and enhanced cold tolerance in plants.
- COR27 /
- Cold treatment /
- Cold tolerance /
- Transgenic Arabidopsis

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