Plant Regeneration from Corm Segments and Evaluations on Physiological and Photosynthetic Characteristics in Plantlets of Red Bud Taro
-
摘要: 以江西铅山红芽芋(Colocasia esculenta L. Schott var. cormosus‘Hongyayu’)试管苗为材料, 建立了芋球茎片两步法离体快繁体系, 并对其再生苗的形态指标、染色体数目、生理和光合特性以及叶绿素荧光特性进行了检测。结果表明:(1)红芽芋球茎片单芽诱导的最佳培养基为MS + KT 2 mg/L + 6-BA 1 mg/L + NAA 0.1 mg/L, 诱导培养30 d后将单芽从球茎片上分离, 再接种到生根培养基(MS + KT 2 mg/L + NAA 0.1 mg/L)上培养30 d即可形成完整植株, 移栽成活率高达98%;(2)由球茎片单芽、丛生芽、不定芽离体快繁获得的红芽芋再生苗在形态指标、叶下表皮气孔参数、染色体数目、生理生化指标以及叶片光合特性参数和叶绿素荧光特性方面均无显著差异。说明红芽芋球茎片两步法离体培养的再生苗繁殖系数高、染色体数目稳定, 该离体快繁体系可应用于江西铅山红芽芋的工厂化生产。Abstract: Using Jiangxi Yanshan red bud taro (Colocasia esculenta L. Schott var. cormosus ‘Hongyayu’) plantlets as materials, an in vitro two-step micro-propagation system for red bud taro corm segments was studied in this paper, and the morphology, cytology, physiology, photosynthetic characteristics and chlorophyll fluorescence characteristics of its plantlets were also observed and detected.Results showed that: (1) The best single bud induction medium for red bud taro corm segments was MS + KT 2 mg/L + 6-BA 1 mg/L + NAA 0.1 mg/L. After 30 days, single buds induced from corm segments were separated and transferred onto the rooting medium (MS + KT 2 mg/L + NAA 0.1 mg/L) and were able to grow into complete plants. After 30 days, red bud taro plantlets were removed and transplanted, and the survival rate reached more than 98%. (2) There were no significant differences in morphological indexes, photosynthetic characteristics, leaf epidermis stomatal parameters, chromosome numbers, physiological and biochemical indexes, photosynthetic characteristics parameters and chlorophyll fluorescence characteristics among regenerated plantlets from single bud corm segments, regenerated plantlets from multiple buds or plantlets regenerated from adventitious buds. Therefore, the two-step micro-propagation in vitro system for red bud taro corm segments was able to ensure genetic stability, and provides a theoretical reference for Jiangxi Yanshan red bud taro factory production.
-
-
[1] 吴才君, 范淑英, 蒋育华, 姚海华, 张安波.芋对斜纹夜蛾的诱集作用[J].生态学杂志, 2004, 23(4):172-174. [2] 姜绍通, 程元珍, 郑志, 潘丽军.红芽芋营养成分分析及评价[J].食品科学, 2012, 33(11):269-272. [3] 肖月土.红芽芋无公害高产栽培技术[J].作物杂志, 2006(2):55-56. [4] 赵国华, 陈宗道, 王赟.芋头多糖的理化性质及体内免疫调节活性研究[J].中国食品学报, 2002, 2(3):21-25. [5] 王瑜, 高畅, 张娜, 梁小璇, 佟立全, 陈智贤, 孟庆繁.芋头多糖的提取及生物活性的研究[J].食品工业科技, 2006, 27(6):73-75. [6] 李火金.铅山红芽芋茎尖脱毒组培繁育及高产栽培[J].中国蔬菜, 2012(3):45-46. [7] 洪森荣, 尹明华, 王艾平.江西铅山红芽芋脱毒苗球茎愈伤组织诱导及其再生体系的建立[J].植物研究, 2013, 33(6):738-745. [8] 张迪, 朱根发, 叶庆生, 陈和明.50份蝴蝶兰种质的染色体数目与倍性分析[J].热带作物学报, 2013, 34(10):1871-1876. [9] 陈佰鸿, 李新生, 曹孜义, 姚庆荣.一种用透明胶带粘取叶片表皮观察气孔的方法[J].植物生理学通讯, 2004, 40(2):215-218. [10] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000. [11] 肖华贵, 杨焕文, 饶勇, 杨斌, 朱英.甘蓝型油菜黄化突变体的光合特性及叶绿素荧光参数分析[J].作物学报, 2013, 39(3):520-529. [12] 柯卫东, 刘玉平, 黄新芳, 彭静.芋组织培养及其相关因素的研究[J].湖北农业科学, 2000(3):47-49. [13] 杨春梅, 单芹丽, 许凤, 李金泽, 曹桦, 汪国鲜, 孟金贵.云南红芋组织培养和快繁技术研究[J].西南农业学报, 2013, 26(2):676-680. [14] 柏新富, 蒋小满, 毕可华, 李明福. 芋脱毒苗的组培快繁及田间试验[J]. 应用与环境生物学报, 2002, 8(1):52-55. [15] 刘独臣, 房超, 李跃建, 刘小俊, 梁根云, 杨宏.芋茎尖离体培养再生体系建立[J].长江蔬菜, 2010(14):30-31. [16] 李伯林, 高成伟, 黄霞.荔蒲芋的组织培养[J].广西师范大学学报:自然科学版, 1998, 16(3):81-84. [17] 徐炯志, 于文进, 龙明华.荔蒲芋组织培养和快速繁殖[J].广西热作科技, 2000(2):19-20. [18] Pradeep CD, Robert MH, Mary T, Anand PT, Douglas KB. Somatic embryogenesis, organoge-nesis and plant regeneration in taro (Colocasia esculenta var. esculenta)[J]. Plant Cell Tiss Org, 2009, 99(1): 61-71.
[19] Chand H, Pearson MN. Rapid vegetative multiplication in Colocasia esculenta (L.) Schott (taro)[J]. Plant Cell Tiss Org, 1998, 55(3): 223-226.
[20] Al Khateeb W, Bahar E, Lahham J, Schroeder D, Hussein E. Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)[J]. Physiol Mol Biol Plants, 2013, 19(1): 157-164.
[21] Lattoo SK, Bamotra S, Sapru DR, Khan S, Dhar AK. Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker—an endangered medicinal herb[J]. Plant Cell Rep, 2006, 25(6): 499-506.
[22] Ahmad N, Javed SB, Khan MI, Anis M. Rapid plant regeneration and analysis of genetic fidelity in micropropagated plants of Vitex trifolia: an important medicinal plant[J]. Acta Physiol Plant, 2013, 35(8): 2493-2500.
[23] Ramakrishnan M, Antony CS, Duraipandiyan V, Ignacimuthu S. Efficient plant regeneration from shoot apex explants of maize (Zea mays) and analysis of genetic fidelity of regenerated plants by ISSR markers[J]. Plant Cell Tiss Org, 2014, 119(1):183-196.
[24] Wang QM, Gao FZ, Gao X, Zou FY, Sui X, Wang M, Hui YJ, Wang L. Regeneration of Clivia miniata and assessment of clonal fidelity of plantlets[J]. Plant Cell Tiss Org, 2012, 109(2): 191-200.
计量
- 文章访问数: 1305
- HTML全文浏览量: 1
- PDF下载量: 1320
下载:
