基于cpDNA和ITS序列对庭藤复合群的居群遗传学研究

彭春巧; 赵雪利; 高信芬

doi:10.11913/PSJ.2095-0837.2015.40425

基于cpDNA和ITS序列对庭藤复合群的居群遗传学研究

彭春巧^1,2,
赵雪利^1,2,
高信芬^1, ,

1.
中国科学院成都生物研究所, 山地生态恢复与生物资源利用重点实验室, 成都 610041
2. 中国科学院大学, 北京 100049

基金项目: 国家自然科学基金面上项目(31370230)。

详细信息

作者简介:
彭春巧(1990-),女,硕士研究生,研究方向为植物生态学(E-mail: chunqiaop@sina.com)。

通讯作者:
高信芬, E-mail: xfgao@cib.ac.cn

中图分类号: Q949.751.9
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出版历程
- 收稿日期: 2015-03-24
- 发布日期: 2015-08-27

Study on the Population Genetics of Indigofera decora Complex Based on cpDNA and nrDNA ITS Sequences

1.
Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2. University of the Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 采用2个叶绿体基因组片段(ndhJ-trnF和trnD-trnT)和核基因组ITS序列对分布于中国东部和相邻地区的庭藤复合群(Indigofera decora complex)的24个居群进行分析,探讨该复合群的遗传多样性、单倍型分布、遗传结构和居群历史动态。cpDNA和ITS序列分析表明,庭藤复合群的单倍型多样性较高,而总体遗传多样性偏低(cpDNA片段: H_d=0.778,π=0.00123;ITS: H_d=0.909,π=0.01290);皖浙地区居群的遗传多样性相对较高,推测其可能是该复合群遗传多样性的分布中心。遗传变异主要存在于复合群居群间,种内居群间遗传分化较大(cpDNA片段: F_ST=0.848;ITS: F_ST=0.787);基因流较小(cpDNA片段: Nm=0.228;ITS: Nm=0.241),主要是地理隔离(遗传漂变)或生境片段化促进了物种形成;复合群的不同物种间存在由基因渐渗引起的单倍型共享现象,可能是种间不完全的生殖隔离所致。花木蓝的居群单独聚为一枝,且与其他种无共享单倍型,因此应保留花木蓝种的分类地位,而其他种的分类地位有待进一步研究。
- 庭藤复合群 /
- ndhJ-trnF /
- trnD-trnT /
- ITS /
- 居群遗传
Abstract: To address questions of interspecific genetic variability and explore the mechanism of speciation in the Indigofera decora complex, a phylogenetic analysis of the complex distributed in eastern and neighboring regions of China was conducted based on cpDNA (ndhJ-trnF and trnD-trnT) and nrDNA (ITS) sequences, including 185 individuals from 24 populations.Results suggested that the Indigofera decora complex had high haplotype diversity and low genetic diversity (cpDNA: H_d=0.778,π=0.00123; ITS: H_d=0.909,π=0.01290).Anhui and Zhejiang were suggested to be the centers of genetic diversity based on the population diversity index.Most genetic variation in the complex was confined to among-population differentiation rather than within-population variation.Intraspecific differentiation among populations was high (cpDNA: F_ST=0.848; ITS: F_ST=0.787), while gene flow was low (cpDNA: Nm=0.228; ITS: Nm=0.241), which showed that isolation-by-distance (genetic shift) or habitat fragmentation promoted species diversification of the complex.Incomplete reproduction isolation of the species resulted in shared haplotypes among species by introgression.The populations of I.kirilowii were diverged from other populations and had no shared haplotypes with other species, thus its taxonomic status should be kept.However, further study is needed on the taxonomic status of other species of the complex.
- Indigofera decora complex /
- ndhJ-trnF /
- trnD-trnT /
- ITS /
- Population genetics

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参考文献(35)

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基于cpDNA和ITS序列对庭藤复合群的居群遗传学研究

作者简介: 彭春巧(1990-),女,硕士研究生,研究方向为植物生态学(E-mail: chunqiaop@sina.com)。

通讯作者: 高信芬, E-mail: xfgao@cib.ac.cn

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出版历程

Study on the Population Genetics of Indigofera decora Complex Based on cpDNA and nrDNA ITS Sequences

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出版历程

目录

作者简介:
彭春巧(1990-),女,硕士研究生,研究方向为植物生态学(E-mail: chunqiaop@sina.com)。

通讯作者:
高信芬, E-mail: xfgao@cib.ac.cn