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一株淡水水华胞甲藻的形态观察和系统发育分析

张琪, 郑凌凌, 李天丽, 宋会银, 刘国祥, 宋立荣

张琪, 郑凌凌, 李天丽, 宋会银, 刘国祥, 宋立荣. 一株淡水水华胞甲藻的形态观察和系统发育分析[J]. 植物科学学报, 2015, 33(4): 458-465. DOI: 10.11913/PSJ.2095-0837.2015.40458
引用本文: 张琪, 郑凌凌, 李天丽, 宋会银, 刘国祥, 宋立荣. 一株淡水水华胞甲藻的形态观察和系统发育分析[J]. 植物科学学报, 2015, 33(4): 458-465. DOI: 10.11913/PSJ.2095-0837.2015.40458
ZHANG Qi, ZHENG Ling-Ling, LI Tian-Li, SONG Hui-Yin, LIU Guo-Xiang, SONG Li-Rong. Morphological Observation and Phylogenetic Analysis of a Freshwater Blooming Cystodinium Strain[J]. Plant Science Journal, 2015, 33(4): 458-465. DOI: 10.11913/PSJ.2095-0837.2015.40458
Citation: ZHANG Qi, ZHENG Ling-Ling, LI Tian-Li, SONG Hui-Yin, LIU Guo-Xiang, SONG Li-Rong. Morphological Observation and Phylogenetic Analysis of a Freshwater Blooming Cystodinium Strain[J]. Plant Science Journal, 2015, 33(4): 458-465. DOI: 10.11913/PSJ.2095-0837.2015.40458
张琪, 郑凌凌, 李天丽, 宋会银, 刘国祥, 宋立荣. 一株淡水水华胞甲藻的形态观察和系统发育分析[J]. 植物科学学报, 2015, 33(4): 458-465. CSTR: 32231.14.PSJ.2095-0837.2015.40458
引用本文: 张琪, 郑凌凌, 李天丽, 宋会银, 刘国祥, 宋立荣. 一株淡水水华胞甲藻的形态观察和系统发育分析[J]. 植物科学学报, 2015, 33(4): 458-465. CSTR: 32231.14.PSJ.2095-0837.2015.40458
ZHANG Qi, ZHENG Ling-Ling, LI Tian-Li, SONG Hui-Yin, LIU Guo-Xiang, SONG Li-Rong. Morphological Observation and Phylogenetic Analysis of a Freshwater Blooming Cystodinium Strain[J]. Plant Science Journal, 2015, 33(4): 458-465. CSTR: 32231.14.PSJ.2095-0837.2015.40458
Citation: ZHANG Qi, ZHENG Ling-Ling, LI Tian-Li, SONG Hui-Yin, LIU Guo-Xiang, SONG Li-Rong. Morphological Observation and Phylogenetic Analysis of a Freshwater Blooming Cystodinium Strain[J]. Plant Science Journal, 2015, 33(4): 458-465. CSTR: 32231.14.PSJ.2095-0837.2015.40458

一株淡水水华胞甲藻的形态观察和系统发育分析

基金项目: 国家高技术研究发展计划(863计划)(2014AA022001)。
详细信息
    作者简介:

    张琪(1985-),男,博士,研究方向为藻类分类和系统发育(E-mail: zhangqiwx@sina.com)。

    通讯作者:

    宋立荣, E-mail: lrsong@ihb.ac.cn

  • 中图分类号: Q949.24

Morphological Observation and Phylogenetic Analysis of a Freshwater Blooming Cystodinium Strain

  • 摘要: 胞甲藻属(Cystodinium Klebs)是植甲藻目(Phytodinales)一种生活于淡水表层、漂浮、不运动的甲藻类群。由于胞甲藻属材料不易获取、人工培养困难等,目前对该属的分类地位、分子系统发育和生活史等方面的了解仍十分有限。2013年夏季,我们从武汉市官桥基地一个发生严重胞甲藻水华的鱼塘中取样,经过分离和培养,于国内首次成功获得此甲藻,将其编号为FACHB-1781,并保存于淡水藻种库中。经形态观察和分子鉴定,确认该甲藻为胞甲藻属的巴达维亚胞甲藻(Cystodinium bataviense Klebs),其形态特征为:营养细胞近新月形或长卵形,两端渐窄、末端钝圆,背腹侧常向外部隆起,无刺或角状延伸。基于SSU rDNA序列的系统发育分析表明,植甲藻目不是单系起源的类群,巴达维亚胞甲藻与Cystodinium phaseolus亲缘关系密切且聚为一枝,而另一些植甲藻类群(如Hemidinium nasutumGloeodinium viscum)与其它已知SSU rDNA序列的甲藻类群亲缘关系不密切。
    Abstract: Many immobile freshwater dinoflagellates, including the epineustonic genus Cystodinium Klebs, belong to the order Phytodinales.Due to limited cultivation and materials, knowledge on the taxonomy, phylogenetic position and life cycle of the genus Cystodinium is limited.An algal bloom was observed in a fish pond in Wuhan, Hubei Province, China in the summer of 2013.Cells were isolated from enriched samples using a micropipette.The clonal culture (FACHB-1781) was established and maintained in the Freshwater Algae Culture Collection at the Institute of Hydrobiology, Chinese Academy of Sciences.The species was identified as Cystodinium bataviense Klebs by morphological and molecular evidence.The vegetative cells were lunate or shaped like an elongated oval with blunt horns.The poles were bluntly prolonged and without spines or projections.Phylogenetic results based on SSU rDNA sequences indicated that the order Phytodinales was not monophyletic.Some Phytodinales groups (e.g.Hemidinium nasutum and Gloeodinium viscum) did not show any consistent affiliation with any other lineage.While C.bataviense clustered with C.phaseolus, the phylogenetic position of the Cystodinium clade was still unknown.Future work on this genus will help clarify its phylogenetic position within the dinoflagellates.
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  • 收稿日期:  2014-12-24
  • 发布日期:  2015-08-27

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