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盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析

杨中敏, 王艳

杨中敏, 王艳. 盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
引用本文: 杨中敏, 王艳. 盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
Citation: YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. DOI: 10.11913/PSJ.2095-0837.2015.40499
杨中敏, 王艳. 盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2015, 33(4): 499-506. CSTR: 32231.14.PSJ.2095-0837.2015.40499
引用本文: 杨中敏, 王艳. 盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2015, 33(4): 499-506. CSTR: 32231.14.PSJ.2095-0837.2015.40499
YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. CSTR: 32231.14.PSJ.2095-0837.2015.40499
Citation: YANG Zhong-Min, WANG Yan. Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress[J]. Plant Science Journal, 2015, 33(4): 499-506. CSTR: 32231.14.PSJ.2095-0837.2015.40499

盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析

基金项目: 国家自然科学基金青年科学基金项目(31400729)。
详细信息
    作者简介:

    杨中敏(1987- ),女,硕士研究生,研究方向为盐生植物抗逆的分子机制(E-mail: yangzhongmin161220@126.com)。

    通讯作者:

    王艳, E-mail: wangyanxju@126.com

  • 中图分类号: Q943.2

Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress

  • 摘要: 利用 RACE技术从盐生植物盐穗木(Halostachys caspica)中克隆获得了一个钾离子转运体基因,经BLAST同源比对发现该基因与拟南芥钾离子转运蛋白AtKUP12基因最为相似,命名为HcKUP12HcKUP12基因cDNA全长为2953 bp,含有2544 bp的阅读框、262 bp的5'-UTR和147 bp的3'-UTR,编码847个氨基酸,分子质量为93.31 kD,理论等电点为7.19。利用实时荧光定量RT-PCR分析了HcKUP12基因在600 mmol/L NaCl处理下胁迫24 h的表达模式,结果显示该基因在盐胁迫下表达量显著增加,至处理24 h时达到最高(为对照的225倍),初步推测其可能是与盐穗木耐盐相关的一个候选基因。
    Abstract: A gene encoded potassium transporter was cloned from Halostachys caspica by SMARTTM RACE, and homology analysis by BLAST showed that it had high identity with AtKUP12 from Arabidopsis thaliana, so named HcKUP12. Results showed that HcKUP12 contained a 262 bp 5'-UTR, 147 bp 3'-UTR and 2544 bp open reading frame encoding 847 amino acids with a molecular weight of 93.31 kD and an isoelectric point of 7.19. The expression profile of HcKUP12 was examined under 600 mmol/L NaCl stress during 24 h using real-time qRT-PCR. Results indicated that its expression level was significantly increased and showed the highest level of expression (225 times higher than that of the control) after 24 h treatment. This suggests that the HcKUP12 gene might be a candidate gene related to salt tolerance in Halostachys caspica.
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出版历程
  • 收稿日期:  2015-02-15
  • 发布日期:  2015-08-27

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