慈姑45S rDNA和端粒序列检测及核型分析

佘朝文; 蒋向辉

doi:10.11913/PSJ.2095-0837.2015.40507

慈姑45S rDNA和端粒序列检测及核型分析

佘朝文^1,2,3, ,,
蒋向辉^1,2,3

1.
怀化学院民族药用植物资源研究与利用湖南省重点实验室, 湖南怀化 418008
2. 怀化学院湘西药用植物与民族植物学湖南省高校重点实验室, 湖南怀化 418008
3. 怀化学院生命科学系, 湖南怀化 418008

基金项目: 湖南省科技计划重点项目(2013FJ4324)。

详细信息

作者简介:
佘朝文(1964-),男,博士,教授,研究方向为植物分子细胞遗传学(E-mail: shechaowen@tom.com)。

通讯作者:
佘朝文, E-mail: shechaowen@tom.com

中图分类号: Q943
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- 收稿日期: 2014-12-16
- 发布日期: 2015-08-27

Detection of 45S rDNA and Telomere Sequences and Karyotype Analysis of Sagittaria trifolia L.

SHE Chao-Wen^1,2,3, ,,
JIANG Xiang-Hui^1,2,3

1.
Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua, Hunan 418008, China
2. Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua, Hunan 418008, China
3. Department of Life Sciences, Huaihua University, Huaihua, Hunan 418008, China

摘要

摘要: 以45S rDNA和拟南芥型端粒序列为探针对慈姑(Sagittaria trifolia L.)有丝分裂中期染色体进行单色和双色荧光原位杂交分析,并用银染方法检测慈姑45S rDNA位点的表达,最后结合染色体测量数据和45S rDNA杂交信号建立慈姑的核型。结果显示,慈姑的单倍基因组总长度为76.9±1.38 μm,最长染色体为 11.55±0.10 μm,最短染色体为 4.54±0.27 μm;慈姑的核型公式为:2n=22=2m + 2sm + 14st + 4t,核型不对称性参数CI、A1、A2、As K(%)、AI分别为19.86±11.06、0.72、0.27、78.82、15.29,核型属于Stebbins类型中的3B型。慈姑具有3对45S rDNA位点,分别位于第8、9、10号染色体的短臂末端。拟南芥型端粒序列的杂交信号出现在慈姑每一条染色体的长、短臂末端。银染检测到6个Ag-NOR和6个核仁,表明3对45S rDNA 位点在间期核都有表达。本研究结果为药食兼用植物慈姑提供了分子细胞遗传学基础资料。
- 慈姑 /
- 核型 /
- 45S rDNA /
- 端粒序列 /
- 荧光原位杂交 /
- 银染
Abstract: To provide basic molecular cytogenetic data for Sagittaria trifolia L., single- and dual-color fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes of S.trifolia with 45S rDNA and Arabidopsis-type telomere sequences were performed, the expression of the 45S rDNA sites was detected using silver staining, and the karyotype of this species was established by combining chromosome measurements and rDNA FISH signals. The mean haploid karyotype length was 76.90±1.38 μm, the longest chromosome pair was 11.55±0.10 μm and the shortest chromosome pair was 4.54±0.27 μm. The karyotype asymmetry indices, CI, A1, A2, As K(%) and AI were 19.86±11.06, 0.72, 0.27, 78.82 and 15.29, respectively, and the asymmetric karyotype belonged to the 3B type of Stebbins' category. Three pairs of 45S rDNA sites were identified and located at the terminals of the short arms of chromosome pairs 8, 9 and 10, respectively. FISH signals from the Arabidopsis-type telomere sequence were observed at the ends of the short and long arms of each chromosome pair. Silver staining showed six Ag-NORs on mitotic chromosomes and one to six nucleoli in interphase cells, indicating that the three pairs of 45S sites were all expressed at interphase.
- Sagittaria trifolia /
- Karyotype /
- 45S rDNA /
- Telomere sequence /
- Fluorescence in situ hybridization (FISH) /
- Silver staining

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参考文献(21)

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慈姑45S rDNA和端粒序列检测及核型分析

作者简介: 佘朝文(1964-),男,博士,教授,研究方向为植物分子细胞遗传学(E-mail: shechaowen@tom.com)。

通讯作者: 佘朝文, E-mail: shechaowen@tom.com

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出版历程

Detection of 45S rDNA and Telomere Sequences and Karyotype Analysis of Sagittaria trifolia L.

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出版历程

目录

作者简介:
佘朝文(1964-),男,博士,教授,研究方向为植物分子细胞遗传学(E-mail: shechaowen@tom.com)。

通讯作者:
佘朝文, E-mail: shechaowen@tom.com