Contents of Flavor Components in Newhall Navel Oranges (Citrus sinensis) Harvested from Eleven Production Areas of China
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摘要: 2011年、2012年从11个脐橙产地收集到22份纽荷尔脐橙果实样品,采用气相色谱法对果实汁胞中的可溶性糖和有机酸进行检测,并采用高效液相色谱法检测2012年果实中的主要苦味物质。结果表明:(1)果实中的可溶性糖主要为果糖、葡萄糖和蔗糖,其中蔗糖含量最高(占可溶性总糖含量的51.01%~60.19%),肌醇含量最低;丹江口、桂林和衢州等不同产地的果实中可溶性糖含量在不同年份间有显著差异;(2)果实中的有机酸主要是苹果酸、柠檬酸和奎宁酸,其中柠檬酸含量最高(占总酸含量的73.74%~90.04%),奎宁酸含量最低;丹江口等产地的果实中有机酸含量在不同年份间有显著差异;(3)果实中的主要苦味物质为柠檬苦素和诺米林,吉首和雅安果实中的柠檬苦素含量(分别为670.0±293.2 μg/g和652.1±217.0 μg/g)显著高于其他产地;衢州果实中的诺米林含量最高(789.7±38.9 μg/g),并显著高于其他产地。对11个产地纽荷尔脐橙果实中的糖、酸与苦味物质进行偏相关分析,结果表明果实中各风味物质具有一定的相关性,其中果糖和葡萄糖、苹果酸和奎宁酸、诺米林和肌醇各含量之间均呈显著正相关,蔗糖与奎宁酸含量呈显著负相关。纽荷尔脐橙果实风味物质在不同产地间有一定差异, 气候因素可能是造成成熟果实酸和苦味物质含量差异的主要原因。Abstract: Gas chromatography (GC) was applied to evaluate the contents of soluble sugars and organic acids in the juice sacs of mature Newhall navel orange fruits from 11 production areas of China in 2011 and 2012. The main bitter compounds, nomilin and limonin, were determined using high performance liquid chromatography (HPLC) in samples harvested in 2012. Results showed that fructose, glucose and sucrose were the three main soluble sugars. Among total soluble sugars, the content of sucrose accounted for 51.01% - 60.19%, ranking the highest, while the content of inositol accounted for 1.61% - 2.14%, ranking the lowest. Generally, the contents of soluble sugars in the juice sacs differed significantly between 2011 and 2012 in Danjiangkou, Guilin and Quzhou production areas. Citrate and malate were the two most abundant organic acids detected. Organic acid content in many production areas, including Danjiangkou, changed significantly between 2011 and 2012. The contents of limonin in the two most abundant production areas, Jishou and Ya'an, were 670.0±293.2 μg/g and 652.1±217.0 μg/g, respectively. The content of nomilin (789.7±38.9 μg/g) in Quzhou was significantly higher than that in the other production areas. Partial correlation analysis showed that the contents of fructose and glucose, malate acid and quinic acid, and nomilin and inositol were positively correlated (P < 0.05), with correlation coefficients of 0.98, 0.43 and 0.48, respectively; while the content of sucrose and quinic acid was negatively correlated, with r=-0.39 (P < 0.05). Our results showed differences between the quality of sugars, organic acids and bitter compounds in mature Newhall navel oranges in different production areas, with climate among the main determinants of organic acid and bitter compound content.
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Keywords:
- Newhall navel orange /
- Soluble sugar /
- Organic acid /
- Limonin /
- Nomilin
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[1] 邓秀新, 彭抒昂. 柑橘学[M]. 北京: 中国农业出版社, 2013: 15-84. [2] Doty TE. Fructose sweetness: a new dimension[J]. Cereal Food World, 1976, 21: 62-63. [3] 牛景, 赵剑波, 吴本宏, 李绍华, 刘国杰, 姜全. 不同来源桃种质果实糖酸组分含量特点的研究[J]. 园艺学报, 2006, 33(1): 6-11. [4] Pangborn RM. Relative taste intensities of selected sugars and organic acids[J]. J Food Sci, 1963, 28: 726-733. [5] Komatsu A, Moricguchi T, Koyama K, Omura M, Akihama T. Analysis of sucrose synthase genes in citrus suggests different role and phylogenetic relationships[J]. J Exp Bot, 2002, 53(366): 61-71. [6] Lowell CA, Tomlison PT, Koch KE. Sucrose-metabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit[J]. Plant Physiol, 1989, 90: 1394-1402. [7] Echeverria E, Gonzalez PC, Brune A. Characteri-zation of proton and sugar transport at the tonoplast of sweet lime (Citrus limmetioides) juice cells[J]. Physiol Plant, 1997, 101(2): 291-30. [8] 陈发兴, 刘星辉, 陈立松. 果实有机酸代谢研究进展[J]. 果树学报, 2005, 22(5): 526-531. [9] 张红艳, 员丽娟, 宋文化, 徐娟. 秭归地区6个夏橙品种果实主要矿质元素含量和糖酸等营养品质的比较[J]. 华中农业大学学报, 2008, 27(4): 522-526. [10] 孙志高,黄学根,焦必宁,王华,郭莉.柑桔果实主要苦味成分的分布及橙汁脱苦技术研究[J].食品科学, 2005, 26(6): 46-148. [11] Bartolozzi F, Bertazza G, Bassi D, Cristoferi G. Simultaneous determination of soluble sugars and organic acids as their trimethylsilyl derivatives in apricot fruits by gas-liquid chromatography[J]. J Chromatography A, 1997, 758: 99-107. [12] 丁帆, 刘宝贞, 邓秀新, 王壮, 谢宗周, 方贻文, 徐娟. 6个甜橙品种果汁的后苦味分析[J]. 华中农业大学学报, 2010, 29(4): 497-501. [13] Albertini MV, Carcouet E, Pailly O, Gambotti C, Luro F, Berti L. Changes in organic acids and sugars during early stages of development of acidic and acidless citrus fruit[J]. J Agric Food Chem, 2006, 54(21): 8335-8339. [14] 龚荣高, 张光伦, 吕秀兰, 曾秀丽, 罗楠, 胡强. 脐橙在不同生境下果实蔗糖代谢相关酶的研究[J]. 园艺学报, 2004, 31(6): 719-722. [15] 邱文伟, 张光伦, 张嵩. 柑橘等果实代谢及其生态调控研究进展[J]. 四川农业大学学报, 2005, 23(1): 114-119. [16] 赵淼, 吴延军, 蒋桂华, 谢鸣, 林毅, 蔡永萍. 柑橘果实有机酸代谢研究进展[J]. 果树学报, 2008, 25(2): 225-230. [17] 龚荣高, 吕秀兰, 张光伦, 曾秀丽, 罗楠, 胡强. 罗伯逊脐橙在不同生境下果实有机酸代谢相关酶的研究[J].果树学报, 2006, 23(6): 805-808. [18] 罗显扬, 金学臣, 刘国斌, 张正秋. 海拔高度对朋娜果实品质的影响[J]. 山地农业生物学报, 2000, 19(1): 33-36. [19] 文涛, 熊庆娥, 曾伟光, 刘远鹏. 气候因子与脐橙果实糖、酸含量的灰色关联度分析[J]. 四川农业大学学报, 2001, 19(3): 225-227. [20] Sun CD, Chen KS, Chen Y, Chen QJ. Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four cultivars during fruit growth and maturation[J]. Food Chem, 2005, 93: 599-605. [21] Li S J, Wang Z, Ding F, Sun D, Ma ZC, Cheng YJ, Xu J. Content changes of bitter compounds in 'Guoqing No.1'Satsuma mandarin (Citrus unshiu Marc.) during fruit development of consecutive 3 seasons[J]. Food Chem, 2014,145: 963-969. [22] 丁帆,刘宝贞,王壮,邓秀新,徐娟.贮藏温度对柑橘果肉中柠檬苦素和柚皮苷含量变化的影响[J].华中农业大学学报,2010,29(2):221-226.
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