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柑橘MYB15基因的克隆与表达分析

郭文芳, 刘德春, 杨莉, 庄霞, 张涓涓, 王书胜, 刘勇

郭文芳, 刘德春, 杨莉, 庄霞, 张涓涓, 王书胜, 刘勇. 柑橘MYB15基因的克隆与表达分析[J]. 植物科学学报, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
引用本文: 郭文芳, 刘德春, 杨莉, 庄霞, 张涓涓, 王书胜, 刘勇. 柑橘MYB15基因的克隆与表达分析[J]. 植物科学学报, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
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GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
Citation: GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. DOI: 10.11913/PSJ.2095-0837.2015.60808
shu
郭文芳, 刘德春, 杨莉, 庄霞, 张涓涓, 王书胜, 刘勇. 柑橘MYB15基因的克隆与表达分析[J]. 植物科学学报, 2015, 33(6): 808-818. CSTR: 32231.14.PSJ.2095-0837.2015.60808
引用本文: 郭文芳, 刘德春, 杨莉, 庄霞, 张涓涓, 王书胜, 刘勇. 柑橘MYB15基因的克隆与表达分析[J]. 植物科学学报, 2015, 33(6): 808-818. CSTR: 32231.14.PSJ.2095-0837.2015.60808
shu
GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. CSTR: 32231.14.PSJ.2095-0837.2015.60808
Citation: GUO Wen-Fang, LIU De-Chun, YANG Li, ZHUANG Xia, ZHANG Juan-Juan, WANG Shu-Sheng, LIU Yong. Cloning and Expression Analysis of MYB15 Genes from Citrus[J]. Plant Science Journal, 2015, 33(6): 808-818. CSTR: 32231.14.PSJ.2095-0837.2015.60808
shu

柑橘MYB15基因的克隆与表达分析

  • 江西农业大学农学院园艺系, 南昌 330045

基金项目: 国家自然科学基金项目(31260468);江西省青年自然科学基金项目(20114BAB214006);江西省自然科学基金项目(20142BAB204008);江西省教育厅科技计划项目(GJJ14292);江西农业大学科学研究基金项目(CX201101)。
详细信息
    作者简介:

    郭文芳(1986-),女,硕士,主要从事果树分子生物学研究(E-mail:guowenfang1986@163.com);刘德春(1982-),男,讲师,主要从事果树分子生物学研究(E-mail:ldc873380800@163.com)。

  • 中图分类号: Q943.2

Cloning and Expression Analysis of MYB15 Genes from Citrus

  • Department of Horticulture, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China

摘要

    摘要
  • 摘要: 采用电子克隆和RT-PCR方法从柚(Citrus maxima (Burm.) Merr.)、枳(Poncirus trifoliata (L.) Raf.)和柠檬(Citrus limon (L.) Burm. f.)实生苗中克隆了3个MYB蛋白基因,分别命名为CmMYB15、PtMYB15和ClMYB15;并用实时定量qRT-PCR技术检测了该基因在脱落酸(ABA)、干旱、低温和高盐胁迫处理下的时空表达。结果显示,CmMYB15、PtMYB15和ClMYB15的cDNA序列全长分别为994、992、988 bp,分别编码267、266、265个氨基酸,且编码的氨基酸序列N端均含有2个串联的不完全重复的MYB DNA-binding结构域,由此推测该3个基因均属于R2R3亚类;MYB15基因均能被ABA、干旱、低温和高盐胁迫诱导表达,且在柚、枳和柠檬中存在表达差异。本研究表明柚CmMYB15、枳PtMYB15和柠檬ClMYB15是MYB基因家族成员,可能在柑橘响应非生物胁迫过程中起到一定的作用。
    Abstract: Three MYB genes, including CmMYB15, PtMYB15 and ClMYB15, were cloned from seedlings of pomelo (Citrus maxima (Burm.) Merr.), trifoliate orange (Poncirus trifoliata (L.) Raf.) and lemon (Citrus limon (L.) Burm.f.), respectively, by in silico and reverse transcription PCR approaches. Results showed that the full length cDNA sequences of the three genes were 994, 992 and 988 bp, and encoded 267, 266 and 265 amino acids, respectively. The N-terminal of the three novel proteins contained two series of incomplete repeat MYB DNA-binding domains, suggesting that the three genes belonged to the R2R3 subclass of the MYB family. Moreover, the expression patterns of these genes were analyzed by real-time quantitative PCR (qRT-PCR) under the stress of ABA, drought, cold and salt.Results indicated that the expressions of the three MYB15 genes were induced by all stress treatments. However, their expression levels after stress differed among the citrus species.The results of this paper suggested that CmMYB15, PtMYB15 and ClMYB15 were members of the MYB gene family, and might play important roles in citrus in response to abiotic stress.
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    • 参考文献(25)
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出版历程
  • 收稿日期:  2015-05-08
  • 发布日期:  2015-12-27

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