‘正午’牡丹微繁殖体系的建立

文书生; 成仿云; 钟原; 王新; 李刘泽木; 黄弄璋

doi:10.11913/PSJ.2095-0837.2016.10143

‘正午’牡丹微繁殖体系的建立

北京林业大学园林学院, 国家花卉工程技术研究中心, 北京 100083

基金项目:

"十二五"农村领域国家科技计划课题(2012BAD01B0704).

详细信息

作者简介:
文书生(1988-),女,博士研究生,研究方向为花卉生物技术(E-mail:shusheng0507@126.com).

通讯作者:
成仿云,E-mail:chengfy8@263.net

中图分类号: Q943.1;S685.11
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出版历程
- 收稿日期: 2015-08-20
- 网络出版日期: 2022-10-31
- 发布日期: 2016-02-27

Protocol for the Micropropagation of Tree Peony (Paeonia × lemoinei ‘High Noon’)

College of Landscape Architecture, Beijing Forestry University, National Flower Engineering Technology Research Center, Beijing 100083, China

Funds:

This work was supported by a grant from the National Science and Technology Support Program of China (2012BAD01B0704).

摘要

摘要: 本研究以‘正午’牡丹腋芽为外植体,建立了高效的牡丹微繁殖体系.腋芽的初始培养基为WPM+ 0.5 mg/L BA+ 0.2 mg/L GA₃,培养50 d 后,一个丛生芽平均可切分为13个繁殖体用于增殖培养;增殖培养基为WPM[1668 mg/L Ca(NO₃)₂·4 H₂O]+ 0.5 mg/L BA+ 0.2 mg/L GA₃,以35 d为一个继代培养周期,增殖率为3.0,共继代培养7次;生根培养时,先将无根苗在复壮培养基[1/2 MS (296 mg/L CaCl₂)+ 0.5 g/L 活性炭]上培养20 d,再转入根诱导培养基[1/2 MS (296 mg/L CaCl₂)+ 1.0 mg/L腐胺+ 1.0 mg/L IBA]培养30 d,最后转入根形成培养基[1/2 MS (296 mg/L CaCl₂)+ 4.0 g/L 活性炭]培养20 d,其生根率达77.2%;驯化与移栽基质为珍珠岩:蛭石:草炭土 = 1:1:1,组培苗移栽成活率高达92.1%.这表明以‘正午’牡丹腋芽建立的微繁殖体系具备规模化商业生产的价值.
- 腋芽 /
- 硝酸钙 /
- 增殖 /
- 复壮培养 /
- 生根 /
- 移栽驯化
Abstract: An efficient micropropagation protocol was developed for Paeonia × lemoinei ‘High Noon’ using axillary buds as explants. During initiation, 13 propagules were obtained after 50 d of culture with WPM + 0.5 mg/L BA + 0.2 mg/L GA₃. During proliferation, three shoots were obtained after 35 d of culture with WPM[1668 mg/L Ca(NO₃)₂·4 H₂O] + 0.5 mg/L BA + 0.2 mg/L GA₃, and seven subcultures were carried out. For optimal rooting, the shoots were cultured with 1/2 MS (296 mg/L CaCl₂) + 0.5 g/L activated charcoal for 20 d, then 1/2 MS (296 mg/L CaCl₂) + 1.0 mg/L putrescine + 1.0 mg/L IBA for 30 d for root induction, and finally 1/2 MS (296 mg/L CaCl₂) ＋ 4.0 g/L activated charcoal for 20 d for root development. The rooting percentage of the shoots was 77.2%. During acclimatization, the rooted plantlets were transferred to pots containing a mix of vermiculite/peat/perlite (1:1:1 V/V/V) substrate, and the survival rate was 92.1%. The micropropagation protocol for Paeonia × lemoinei ‘High Noon’ established in this study is valuable for commercial use.
- Axillary buds /
- Ca(NO₃)₂ܪH₂O /
- Proliferation /
- Rejuvenation culture /
- Rooting /
- Transplantation and acclimatization

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