Anatomical Structure of Vegetative Organs of Ginkgo biloba L.
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摘要: 为了揭示不同树龄银杏的根、茎、叶解剖结构以及内生菌分布情况,本研究采用石蜡切片法对银杏(Ginkgo biloba L.)根、茎、叶显微解剖结构进行了观察。结果显示:(1)一年生银杏幼根不含树脂道,内生菌含量低,而皮层中含有大量蛋白细胞;多年生银杏老根含有较多树脂道,皮层细胞中含有大量内生菌并有针晶物质分布,未发现蛋白细胞。(2)一年生银杏幼茎有明显的角质层,皮层分布有大量蛋白细胞,韧皮射线及髓部发达,其中髓由大量薄壁细胞构成并且有蛋白细胞分布,未观察到树脂道但有簇晶物质存在。(3)多年生银杏叶片海绵组织疏松,具有树脂道,叶肉细胞含有簇晶物质;气孔下陷并具有耐旱的结构特点。本结果可为研究不同树龄银杏对环境的适应性变化以及内生菌特点提供参考。Abstract: To confirm the anatomical structure and distribution of endophytes in the vegetative organs of different aged ginkgoes, we observed the anatomical structure of Ginkgo biloba L.roots, stems and leaves by paraffin section. Results showed that:(1) The root of annual G. biloba did not have a resin canal, but did have a few endophytes and a large number of albuminous cells in the cortex cells. The perennial root had a resin canal and many endophytes, as well as needle-like crystals, but did not have albuminous cells. (2) The young stem had an obvious cuticle, numerous albuminous cells in the cortex cells, and a developed phloem ray and central pith. The latter consisted of a large number of parenchyma cells, with albuminous cells and crystal druses but without a resin canal in the pith. (3) The spongy tissue of G. biloba leaves had a loose structure. There were resin canals and crystal druses in the mesophyll cells, and stomata with invagination under the epidermis, indicating that G. biloba had anatomical characteristics for drought tolerance. Based on the structure of the vegetative organs, this study helps clarify the relationship between different aged G. biloba and the ecological environment and provides reference for the further study of G. biloba endophytes.
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Keywords:
- Ginkgo biloba /
- Anatomical structure /
- Endophytic /
- Crystal druse /
- Albuminous cell /
- Resin canal
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[1] 胡云虹, 彭斌, 齐小晴, 刘学群, 余光辉. 银杏叶愈伤组织和叶中超氧化物歧化酶、总黄酮类抗氧化活性剂活性的比较分析[J].武汉植物学研究, 2010, 28(4):521-526. Hu YH, Peng B, Qi XQ, Liu XQ, Yu GH. Comparative assay of superoxide dismutase and total flavone antioxidant activities in leaf-callus and leaves of Ginkgo biloba L.[J].Journal of Wuhan Botanical Research, 2010, 28(4):521-526.
[2] 郑鹏, 史红文, 邓红兵, 廖建雄. 武汉市65个园林树种的生态功能研究[J].植物科学学报, 2012, 30(5):468-475. Zheng P, Shi HW, Deng HB, Liao JX. Study on the ecological functions of sixty-five garden species in Wuhan city, China[J].Plant Science Journal, 2012, 30(5):468-475.
[3] Liao YL, Xu F, Huang XH, Zhang WW, Cheng H, Wang XH, Cheng SY, Shen YB. Characterization and transcri-ptional profiling of Ginkgo biloba mevalonate diphosphate decarboxylase gene (GbMVD) promoter towards light and exogenous hormone treatments[J].Plant Mol Biol Rep, 2015, DOI: 10.1007/s11105-015-0947-x.
[4] Xu Y,Wang GB, Cao FL, Zhu CC, Wang GY, Yousry AE. Light intensity affects the growth and flavonol biosynthesis of Ginkgo (Ginkgo biloba L.)[J].New Forests, 2014, 45(6):765-776.
[5] 邢世岩, 孙霞, 李可贵,有祥亮. 银杏叶生长发育规律的研究[J].林业科学, 1997, 33(3):267-273. Xing SY, Sun X, Li KG, You XL.Growth and development of Ginkgo biloba Leaves[J].Scientia Silvae Sinicae,1997, 33(3):267-273.
[6] 陈立群, 李承森. 银杏(Ginkgo biloba)叶表皮特征及其气孔的发育[J].植物研究,2004, 24(4):417-422. Chen LQ, Li CS.The epidermal characters and stomatal development of Ginkgo biloba[J].Bulletin of Botanical Research, 2004, 24(4):417-422.
[7] 韩彪, 陈国祥, 高志萍, 魏晓东, 解凯彬, 杨贤松, 唐加红, 刘丹. 两个银杏品种叶片衰老过程中叶绿体功能特性的比较[J].南京师大学报:自然科学版, 2009, 32(3):99-103. Han B, Chen GX, Gao ZP, Wei XD, Xie KB, Yang XS, Tang JH, Liu D. The comparison of chloroplasts function characteristics during leaf senescence in two Ginkgo varie-ties[J].Journal of Nanjing Normal University:Natural Science Edition, 2009, 32(3):99-103.
[8] 王扬, 房荣春, 林明明, 陆彦, 王莉, 金飚. 银杏叶发育过程的解剖结构观察[J].西北植物学报, 2011, 31(5):861-867. Wang Y, Fang RC, Lin MM, Lu Y, Wang L, Jin B. Anatomical structure dynamics of Ginkgo biloba L.leaves during annual growth and development[J].Acta Bot Boreal, 2011, 31(5):861-867.
[9] 张海龙, 李善春, 卢维浩, 雷燕萍, 曹福亮, 曹慧. 银杏内生真菌多样性与产黄酮类物质真菌的分离和鉴定[J].土壤, 2015, 47(1):135-141. Zhang HL, Li SC, Lu WH, Lei YP, Cao FL, Cao H. Distribution and identification of flavonoid-producing endophytic fungi in Ginkgo biloba L.[J].Soils, 2015, 47(1):135-141.
[10] 赵庆云. 产黄酮银杏内生菌的分离、纯化与液态培养[D].杨凌:西北农林科技大学, 2007. Zhao QY. Isolation and optimization of flavone-producing endophytes from Ginkgo biloba. L.[D].Yangling:Northwest A&F University, 2007. [11] Thongsandee W, Matsuda Y, Ito S. Temporal variations in endophytic fungal assemblages of Ginkgo biloba L.[J].J For Res, 2012, 17(17):213-218.
[12] Qiu M, Xie RS, Shi Y, Zhang HH, Chen HM. Isolation and identification of two flavonoid-producing endophytic fungi from Ginkgo biloba L.[J].Ann Microbiol, 2010, 60(1):143-150.
[13] 申屠旭萍, 俞晓平. 银杏中抗病原真菌的内生真菌的分离筛选[J].浙江农业学报, 2006, 18(5):317-320. Sentu XP, Yu XP. Endophytic fungi with anti-pathogenic fungi activities isolated from Gingkgo[J].Acta Agriculturae Zhejiangensis,2006, 18(5):317-320.
[14] Zheng YK, Qiao XG, Miao CP, Liu K, Chen YW, Xu LH, Zhao LX. Diversity, distribution and biotechnological potential of endophytic fungi[J].Ann Microbiol, 2015, DOI: 10.1007/s13213-015-1153-7.
[15] 刘小莉. 银杏内生真菌分离鉴定及其抗菌抗氧化作用研究[D].南京:南京农业大学, 2007. Liu XL. Isolation and identification of endophytic fungi from Ginkgo biloba and study on antimicrobial and anttioxidant activity[D].Nanjing:Nanjing Agricultural University, 2007.
[16] 赵凤娟. 银杏演化结构研究[D].长春:东北师范大学, 2004. Zhao FJ. The research in evolution structure for Ginkgo biloba[D].Changchun:Northeast Normal University, 2004.
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