Growth and Physiological Changes in Amorpha fruticosa Linn. Seedlings Following Paclobutrazol Treatment
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摘要: 采用盆栽试验的方法研究了土施不同浓度多效唑(1、5、10、15、20 mg/L)对紫穗槐(Amorpha fruticosa Linn.)生长及生理特性的影响,以探明多效唑对紫穗槐的作用机制和最佳处理方式。结果显示:随着多效唑处理浓度(1~20 mg/L)的增加,紫穗槐幼苗株高、单叶面积和主根长呈下降趋势,基径、叶片长宽比、根鲜重和根冠比呈上升趋势;多效唑各处理均使紫穗槐幼苗叶片的相对含水量、叶绿素、可溶性糖和可溶性蛋白含量显著升高,POD活性显著增加,MDA含量和相对电导率显著下降。采用隶属函数法对各项生长、生理指标进行综合评价,结果发现20 mg/L多效唑处理下紫穗槐幼苗的抗性最强。说明多效唑可通过调节紫穗槐幼苗的生物量分配、水分状况、细胞渗透性和抗氧化性等,有效改善其生长、生理特性及提高抗逆性。本研究结果为多效唑在边坡植被建成和恢复中的应用提供了理论依据。Abstract: Using pot experiments, the growth and physiological changes in Amorpha fruticosa Linn. seedlings following treatment with different paclobutrazol (PP333) concentrations (1, 5, 10, 15 and 20 mg/L) were investigated to clarify the mechanism and best treatment. In the range of 1-20 mg/L, with the increase in PP333 concentration, tree height, leaf area and taproot length showed a declining trend, whereas base diameter, leaf length to width ratio, fresh weight of root, and root-shoot ratio showed an increasing trend. Relative water content, chlorophyll, soluble sugar and soluble protein content in leaves were effectively increased, POD activity was effectively enhanced, and MDA content and relative conductivity were effectively reduced compared with that in the control. Using the membership function to evaluate the regulation effect of each treatment, A. fruticosa seedlings had the strongest resistance with 20 mg/L PP333. Our study indicated that, by adjusting biomass allocation, moisture content, cell permeability and oxidation resistance of A. fruticosa seedlings, PP333 effectively improved growth and physiological characteristics, and thus the resistance of A. fruticosa seedlings was improved. This study offers a theoretical basis for the application of PP333 in plant and vegetation slope restoration.
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Keywords:
- Amorpha fruticosa Linn. /
- Paclobutrazol /
- Physiological characteristics /
- Resistance
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