不同培养模式下魏氏真眼点藻生长和产油特性的研究

何思思; 高保燕; 黄罗冬; 李爱芬; 张成武

doi:10.11913/PSJ.2095-0837.2016.20289

不同培养模式下魏氏真眼点藻生长和产油特性的研究

暨南大学水生生物研究中心, 生态学系, 广州 510632

基金项目:

国家自然科学基金项目(31170337)

国家高技术研究发展计划"863"项目(2013AA065805)

广东省低碳专项(2011-051)

珠海市科技重大项目(PB20041018)

珠海市科技攻关项目(PC20081008)

详细信息

作者简介:
何思思(1990-),女,硕士研究生,研究方向为微藻生物技术和分子生物学(E-mail:zjhesisi@163.com)。

通讯作者:
张成武(1963-),男,教授,博士生导师,研究方向为微藻生物学和生物技术.E-mail:tzhangcw@jnu.edu.cn

中图分类号: Q947;Q949.2
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出版历程
- 收稿日期: 2015-09-17
- 修回日期: 2015-10-26
- 网络出版日期: 2022-10-31
- 发布日期: 2016-04-27

Growth and Oil-production Characteristics of Eustigmatos vischeri Hibberd under Different Cultivation Modes

Department of Ecology, Research Center for Hydrobiology, Jinan University, Guangzhou 510632, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China(31170337), National High Technology Research and Development Program of China(863 Program, 2013AA065805), Special Program for Low-Carbon, Reform and Development Commission of Guangdong Province(2011-051), Major Scientific and Technical Program of Zhuhai City(PB20041018), and Scientific and Technical R & D Program of Zhuhai City(PC20081008).

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摘要

摘要: 为了解魏氏真眼点藻(Eustigmatos vischeri Hibberd)的生物学特性,探究"批量法"、"两步法"、"补料法"和"添加碳酸氢盐"4种不同培养模式对魏氏真眼点藻生长和油脂积累的影响,本文分别采用不同初始浓度的硝酸钠供应、更换培养基、分次少量补加硝酸钠及添加低浓度NaHCO₃或NH₄HCO₃等方法培养魏氏真眼点藻。结果显示,"批量"培养下,硝酸钠浓度为3.0 mmol/L时藻细胞生物量达到8.41 g/L,油脂最高可达到65.16%,油脂产率为0.30 g·L^-1·d^-1。"两步法"和"补料法"培养对藻细胞油脂积累没有显著影响,而通过"添加碳酸氢盐"培养对该藻细胞生长和油脂积累的效果最显著,其中NaNO₃+NH₄HCO₃组生物量达到11.56 g/L,油脂最高达60.92%,与相同氮浓度"批量"培养相比,生物质浓度提高了1.0 g/L,总脂含量提高了10%,大大提高了该藻的总脂产率(达到0.39 g·L^-1·d^-1)。因此,魏氏真眼点藻是一株高产油藻株,当添加低浓度碳酸氢铵时最有利于促进该藻生物质浓度和总脂含量的提高,这是一种最佳的培养模式,具有潜在的开发和利用价值。
- 魏氏真眼点藻 /
- 培养模式 /
- 生物质浓度 /
- 总脂产率 /
- 碳酸氢盐
Abstract: We investigated the biological characteristics of the alga Eustigmatos vischeri Hibberd, and explored the effects of four cultivation modes (batch, two-stage, fed-batch and bicarbonate-addition culture) on its growth and lipid accumulation. We cultured E. vischeri by supplying different initial sodium nitrate, which was replaced with fresh medium, with a small amount of sodium nitrate and low concentrations of sodium bicarbonate or ammonium bicarbonate added. The biomass concentration in the batch culture reached 8.41 g/L, while the highest total lipid content was 65.16% and productivity was 0.30 g·L^-1·d^-1. Compared to the batch culture, the two-stage and fed-batch cultures had no significant effect on lipid accumulation in E. vischeri. However, the bicarbonate-addition culture did have a significant effect on growth and lipid accumulation, especially maximum biomass concentration in the NaNO₃+NH₄HCO₃ group, which reached 11.56 g/L, and total lipid content reached 60.92%. Thus, the biomass concentration and total lipid content increased by almost 1.0 g/L and 10%, respectively, compared with that in the batch culture under the same nitrogen concentration. In addition, the productivity of total lipids also increased (0.39 g·L^-1·d^-1). Our results indicated that E. vischeri is an oleaginous microalga with high development and utilization value. Moreover, the addition of a low concentration of ammonium bicarbonate promoted the growth and lipid accumulation of E. vischeri, and is thus a suitable method for cultivation.
- Eustigmatos vischeri Hibberd /
- Cultivation modes /
- Biomass concentration /
- Productivity of total lipids /
- Bicarbonate

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参考文献(19)

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