Effects of Spraying Cytokinin on Abscission Rate and Enzymic Activity of Flowers and Pods of Cowpea
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摘要: 以4个豇豆(Vigna unguiculata Linn.)品种‘鄂豇豆6号’、‘鄂豇豆2号’、‘鄂豇豆7号’和‘美国地豆’为材料,在现蕾期叶面喷施植物细胞分裂素(CTK),于喷施后第7、14、28、42d测定脱落花荚的多聚半乳糖醛酸酶(PG)和纤维素酶活性,并统计花荚脱落率和豇豆产量,研究CTK在豇豆生长发育过程中对花荚脱落的影响。结果显示,喷施CTK后,豇豆各品种的花荚脱落率均小于对照,豇豆产量均高于对照,且差异极显著(P<0.01);喷施CTK后第14、28、42d豇豆各品种脱落花荚的PG活性显著降低(P<0.05);喷施CTK后第7d各处理组脱落花荚的PG活性极显著降低(P<0.01);喷施CTK后第7d和第42d豇豆各品种脱落花荚的纤维素酶活性显著降低(P<0.05),喷施CTK后第14d和第28d各处理组脱落花荚的纤维素酶活性极显著降低(P<0.01)。研究结果表明喷施CTK可调节脱落花荚的PG活性和纤维素酶活性,从而降低花荚脱落率,实现对豇豆产量的调控。Abstract: Four cowpea varieties, ‘E Jiangdou 2’, ‘E Jiangdou 6’, ‘E Jiangdou 7’, and ‘Meiguo Didou’, provided by the Hubei Province Engineering Research Center were selected as research materials. We sprayed cytokinin on leaves in budding stage to study its effects on the abscission of flowers and pods in the field. At 7, 14, 28 and 42 d after spraying, we analyzed the yields and abscission rates of flowers and pods, and detected the enzyme activities of polygalacturonase and cellulase in premature shedding of flowers and pods.Results showed that after spraying cytokinin, the abscission rates of flowers and pods in different cultivars were lower than those in the control, but yields in different cultivars were higher, with the differences being highly significant (P < 0.01). Furthermore, the polyga-lacturonase activities of cowpea were significantly lower than those of the control at 14 d, 28 d and 42 d after spraying (P < 0.05), and significantly lower at 7 d after spraying (P < 0.01). The cellulase activities of fallen flowers and pods were significantly (P < 0.05) lower than those of the control at 7 d and 42 d after spraying, and significantly (P < 0.01) lower at 14 d and 28 d after spraying. The above results indicated that the activities of polygalacturonase and cellulase were regulated by spraying cytokinin, which resulted in reducing the abscission rate of flowers and pods and regulating yield in cowpea.
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Keywords:
- Cowpea /
- Cytokinins /
- Flowers and pods /
- Abscission rate /
- Polygalacturonase /
- Cellulase
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[1] 党江波,李彩,李晔,龙志坚,魏旭,汪卫星,何桥,郭启高,梁国鲁. 豇豆螺旋茎段维管组织观察及其成因分析[J]. 植物科学学报,2011, 29(6) : 743-748. Dang JB, Li C, Li Y, Long ZJ, Wei X, Wang WX, He Q, Guo QG, Liang GL. Observation and genesis analysis of vascular tissue in cowpea's helix stem[J]. Plant Science Journal,2011, 29(6) : 743-748.
[2] Taylor JE, Whitelaw CA. Signals in abscission[J]. New Phytologist, 2001, 151(2): 323-339.
[3] Clements JC, Atkins CA. Characterization of a nonab-scission mutant in Lupinus angustifolius L.: Physiological aspects[J]. Annals of Botany, 2001, 88(4) : 629- 635.
[4] Bonghi C, Rascio N, Ramina A, Casadoro G. Cellulase and polygalacturonase involvement in the abscission of leaf and fruit explants of peach[J]. Plant Mol Biol, 1992, 20(5): 839- 848.
[5] 崔洪秋,冯乃杰,孙福东, 刘涛, 李建英,杜吉到,韩毅强,郑殿峰. DTA-6 对大豆花荚脱落纤维素酶和 GmAC基因表达的调控[J].作物学报, 2016, 42(1): 51- 57. Cui HQ, Feng NJ, Sun FD, Liu T, Li JY, Du JD, Han YQ, Zheng DF. Regulation of DTA-6 by abscission cellulase and GmAC gene expression in flowers and pods of soybean[J]. Acta Agronomica Sinica, 2016, 42(1): 51-57. [6] Kemmerer EC,Tucker ML.Comparative study of cellulase associated with adventitious root initiation,apical buds and leaf flower and pod abscission zones in soybean[J].Plant Physiol, 1994,104(2): 557-562.
[7] 李辉亮.激素和热激对大豆(Glycine max (L.) Merrill)花荚脱落的影响及其分子机理研究[D].长沙: 湖南农业大学, 2004. Li HL. Studies on the effects of hormone and heat shock on soybean (Glycine max (L.) Merrill) legume abscission and its molecule mechanism[D]. Changsa: Hunan Agricultural University, 2004.
[8] 刘大林,谷文英,秦玉玲,吕勇,吴敏,王留香. 外源细胞分裂素对紫花苜蓿生长及品质的影响[J]. 草业科学,2005, 22(10):36-40. Liu DL, Gu WY, Qin YL, Lü Y, Wu M, Wang LX. Effects of exogenous CTK on the growth and character of alfalfa (Medicago sativa) plants[J]. Pratacultural Science, 2005, 22 (10):36-40.
[9] 高金山,边庆华,张永忠,王敏. 细胞分裂素CPPU的研究进展[J],农药,2006,45(3): 151-154. Gao JS, Bian QH, Zhang YZ, Wang M. Research progress on the cytokinin, CPPU[J]. Agrochemicals, 2006, 45(3): 151-154.
[10] 宋莉萍,刘金辉,郑殿峰,冯乃杰. 不同时期叶喷植物生长调节剂对大豆花荚脱落率及多聚半乳糖醛酸酶活性的影响[J]. 植物生理学报,2011, 47(4): 356-362. Song LP, Liu JH, Zheng DF, Feng NJ. Effects of different plant growth regulators on abscission rate and polygalacturonase activities of soybean flowers and pods by spraying in different stages[J]. Plant Physiology Journal, 2011, 47(4): 356-362.
[11] 汪宝卿,慈敦伟,张礼凤,李伟,徐冉.同化物供应和内源激素信号对大豆花荚发育的调控[J]. 大豆科学, 2010, 29 (5):878-882. Wang BQ, Ci DW, Zhang LF, Li W, Xu R. Research progress of assimilation supply and endogenous hormones signals regulation involved in flower and pod deve-lopment of soybean[J]. Soybean Science, 2010, 29 (5):878-882.
[12] 赵双进,唐晓东,赵鑫,冯燕,赵聪聪,张孟臣. 大豆开花落花及时空分布的观察研究[J].中国农业科学, 2013, 46(8): 1543-1554. Zhao SJ, Tang XD, Zhao X, Feng Y, Zhao CC, Zhang MC. Observation and research on the temporal and spatial distribution of flowering and flower dropping of soybean[J]. Scientia Agricultura Sinica, 2013, 46(8): 1543-1554.
[13] 孙福东.两种调节剂对无限结荚习性大豆花荚建成的调控及机制[D].大庆市: 黑龙江八一农垦大学,2015. Sun FD. Mechanism and regulation of two kind of plant growth regulators on flowers and pods formation of indeterminate growth habit soybean[D]. Daqing: Heilongjiang Bayi Agricultural University, 2015. [14] Oko BFD, Eneji AE, Binang W,Irshad M, Yamamoto S, Honna T, Endo T. Effect of foliar application of urea on reproductive abscission and grain yield of soybean[J]. J Plant Nutr, 2003, 26 (6) :1223-1234.
[15] 柴国华. 激素、逆境对大豆脱落纤维素酶基因表达的调控及其对花荚脱落的影响[D]. 杨凌:西北农林科技大学, 2006. Cai GH. Regulation of abscission cellulose gene expression and effect of flowers and pods abscission in soybean by hormones or unfavorable circumstances[D].Yangling: Northwest Agricultural and Forestry University, 2006.
[16] 张海峰, 张明才,翟志席, 段留生,李召虎,黄淮,陈刚. SHK-6对不同群体下大豆花荚脱落及其产量的调控[J]. 大豆科学,2007, 26(1):78-83. Zhang HF, Zhang MC, Zhai ZX, Duan LS, Li ZH, Huang Z, Chen G. Effect of SHK-6 application on abscission of flower and pod and yield in different density[J]. Soybean Science, 2007, 26(1):78-83.
[17] 宋莉萍,刘金辉,郑殿峰,冯乃杰. 不同时期PGRs对大豆花荚脱落率及纤维素酶活性的影响[J]. 中国油料作物学报,2011, 33(3):253-258. Song LP, Liu JH, Zheng DF, Feng NJ. Regulation of plant growth regulators on abscission rate and cellulose activity of soybean flowers and pods[J]. Chinese Journal of Oil Crop Sciences, 2011, 33(3):253-258.
[18] 尹宝重, 刘盼, 张月辰. 红小豆花器官脱落对短日照诱导的生理响应[J]. 作物杂志, 2015(1): 78-85. Yin BZ, Liu P, Zhang YC. The physiological response of floral organ abscission for adzuki bean (Vigna angularis) under short daylenth photoperiod induction[J]. Crops, 2015(1): 78-85.
[19] 许涛.钙素对番茄花柄外植体脱落调控机理的研究[D].沈阳: 沈阳农业大学,2008. Xu T. Study on regulating mechanism of calcium on the abscission of tomato pedicel[D]. Shenyang: Shenyang Agricultural University, 2008.
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