Transcriptional Expression of the StSnRK2 Gene Family in Response to Osmotic Stress and Correlation Analysis Between Physiological Traits
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摘要: 蔗糖非酵解相关蛋白激酶家族2（Sucrose Non-Fermenting Related protein Kinases 2，SnRK2）是一类植物中高度保守的蛋白激酶，也是植物响应干旱、盐碱等导致渗透性胁迫的主要调控原件。本研究以马铃薯品种‘陇薯3号’为材料，观察试管苗在0、25、50、100、200 mmol/L NaCl 2周、4周和6周盐胁迫下和0、2%、4%、6%、8% PEG 2周、4周和6周干旱胁迫下马铃薯地上部分组织中StSnRK2基因的表达模式。结果显示，盐和干旱胁迫下马铃薯StSnRK2基因表达模式不尽相同：在盐胁迫下StSnRK2.4 和StSnRK2.6表达均上调；干旱胁迫下StSnRK2.3的表达量上升，而且随PEG浓度的增加StSnRK2.3表达量也随之增高；同一基因在不同胁迫处理下表达趋势也有差异，StSnRK2.5基因在盐胁迫下表达下调，在干旱胁迫下StSnRK2.5基因表达量高于对照；不同胁迫处理下基因的表达与生理指标的相关性也不同，盐胁迫下，StSnRK2.6基因与CAT和SOD活性呈极显著正相关，与气孔面积呈显著负相关，干旱胁迫下，StSnRK2.6基因的表达量与脯氨酸含量呈显著正相关。本文通过研究不同渗透胁迫条件下StSnRK2基因的表达模式，进一步解析了马铃薯对盐及干旱胁迫响应的机理，可为马铃薯抗逆品种的选育提供依据。Abstract: Sucrose non-fermenting-1 related protein kinases 2 (SnRK2) is a family of highly conserved plant protein kinases. They play important roles in abscisic acid-dependent plant development, and are involved in the stress responses of plants to osmotic stress caused by drought and salinity. In the current study, the transcriptional expression of StSnRK2genes from tetraploid potato (Solanum tuberosum L. cv.‘Longshu No.3’) plantlets in response to gradient saline stress (0, 25, 50, 100 and 200 mmol/L NaCl) and gradient water stress (0, 2%, 4%, 6% and 8% PEG) were determined over three continuous observations (two, four, and six weeks, respectively). Results showed that the expression pattern of StSnRK2 varied under salt and drought stresses. The transcripts of StSnRK2.4and StSnRK2.6 increased under saline treatment, whereas StSnRK2.3 increased with PEG treatment. The transcriptional expression of StSnRK2.5 decreased under saline treatment, but increased under PEG treatment. Under saline treatment, the expression of StSnRK2.6 was positively correlated with the activity of CAT and SOD, and negatively correlated with stomatal area. Under PEG treatment, the StSnRK2.6 transcripts were positively correlated with proline content. Results from this study will offer valuable reference for the molecular breeding of stress resistant potato varieties.
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Keywords:
- Potato /
- Osmotic stress /
- StSnRK2 /
- Transcriptional expression /
- Correlation
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