Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis
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摘要: 以黄酮提取率为指标，采用超声辅助法研究了芒草中黄酮类化合物的提取工艺。最佳提取条件为：乙醇浓度70%、料液比1：30、提取温度70℃、提取时间40 min、提取级数3次，在此条件下测得南荻中总黄酮含量为7.24 mg/g，并在此条件下测定了芒草10个品系中总黄酮含量为4.16～7.84 mg/g，且在不同品系间的差异均达到极显著水平。采用气质联用技术鉴定奇岗六倍体、‘GMG121’、芒杂中的挥发油成分，从中共鉴定出28种成分。本研究对芒属植物的遗传育种以及开发和综合利用具有重要意义。
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关键词:
- 芒草 /
- 黄酮含量 /
- 挥发油成分 /
- 超声提取 /
- 气相色谱-质谱联用技术
Abstract: An ultrasonic assisted method was used to study the optimum extraction of flavonoids in Miscanthus. The determined optimal extraction conditions of flavonoids were as follows:70% ethanol concentration, 1:30 ratio of raw material to liquid, 70℃ extraction temperature, 40 min extraction time, and three extract series, respectively. Under these conditions, total flavonoid content of M.lutarioriparius was 7.24 mg/g. The experimental yields of flavonoids from various kinds of M.sinensis were significantly different, ranging from 4.16 mg/g to 7.84 mg/g. The volatile oil components in Miscanthus x giganteus (hexaploid),‘GMG121’, and M.sinensis hybrid were determined by GC/MS, with 28 different components identified. This study provides useful information on genetic breeding, which has great significance for the development and utilization of M.sinensis. -
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