SSR分子标记在山茶属观赏资源遗传多样性研究中的应用

张亚利; 宋垚; 奉树成

doi:10.11913/PSJ.2095-0837.2016.50755

SSR分子标记在山茶属观赏资源遗传多样性研究中的应用

上海植物园, 上海 200231

基金项目: 上海市科委科技支撑项目（15391900300）。

详细信息

作者简介:
张亚利(1979-),女,博士,高级工程师,研究方向为束花茶花种质资源创新与应用(E-mail:shbg2011@126.com)。

通讯作者:
奉树成(E-mail:846058770@qq.com)。

中图分类号: Q943.2
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- 文章访问数: 1362
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出版历程
- 收稿日期: 2016-03-15
- 修回日期: 2016-05-04
- 发布日期: 2016-10-27

Application of SSR for the Analysis of Genetic Diversity in Camellia

i>Shanghai Botanical Garden, Shanghai 200231, China

Funds: This work was supported by a grant from Science and Technology Commission of Shanghai Municipality (15391900300).

摘要

摘要: 采用微卫星（SSR）分子标记的方法，利用20对SSR引物对33份山茶属资源（含种和品种）的DNA样品进行了PCR扩增，从中筛选出10对扩增效果较好的SSR引物，并对33份资源进行了遗传多样性分析。结果显示：10对引物在33个植物材料中共检测出123个等位基因，每个SSR位点的等位基因数为3~22个，SSR2引物检测到的等位基因数量最多，达22个，平均每对引物含有12.3个等位基因，平均多态性信息量为0.74，变化范围为0.06~0.96。利用遗传距离矩阵按UPGMA方法进行聚类，结果表明33份植物材料可分为9个分支，很好的区分了品种间的亲缘关系，可为杂交育种的组合选择提供理论基础。不同花型的山茶属植物在10个微卫星位点上共发现42个特异等位基因，可能与不同的花型性状有关。
- 茶花 /
- SSR /
- 多样性分析
Abstract: In this paper, SSR molecular marker and composite sampling approaches were employed to perform PCR amplification for 33 DNA samples of Camellia. 10 SSR primer pairs that produced good amplification patterns from a total of 20 SSR primer pairs were screened and the genetic diversities and correlations among the 33 Camellia varieties were analyzed. A total of 123 alleles were detected in the 33 Camellia varieties from the 10 screened primer pairs. The number of alleles at each SSR locus ranged from 3 to 22, with a mean of 12.3; the average PIC was 0.76, ranging from 0.06 to 0.96. The highest number of alleles detected by primer SSR2 was 22. By employing the genetic similarity matrix, in combination with UPGMA cluster analysis, the 33 Camellia could be classified into 9 groups, which showed good phylogenetic relationships and provided a theoretical foundation for enhancing breeding efficiency. In addition, 42 special alleles on 10 microsatellite loci from different flower types of Camellia were detected, which might be related to traits of Camellia flower type.
- Camellia /
- SSR /
- Genetic diversity

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SSR分子标记在山茶属观赏资源遗传多样性研究中的应用

作者简介: 张亚利(1979-),女,博士,高级工程师,研究方向为束花茶花种质资源创新与应用(E-mail:shbg2011@126.com)。

通讯作者: 奉树成(E-mail:846058770@qq.com)。

计量

出版历程

Application of SSR for the Analysis of Genetic Diversity in Camellia

计量

出版历程

目录

作者简介:
张亚利(1979-),女,博士,高级工程师,研究方向为束花茶花种质资源创新与应用(E-mail:shbg2011@126.com)。

通讯作者:
奉树成(E-mail:846058770@qq.com)。