nrDNA ITS sequence analysis and genetic relationship of Allium macrostemon from different geographical regions in Guizhou
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摘要: 采用PCR直接测序法，对产自贵州11个不同地区的薤白（Allium macrostemon Bunge）18个样品进行核糖体DNA ITS序列测定，并结合GenBank中下载的来自四川汶川、陕西汉中以及韩国的薤白ITS序列进行对比分析。结果显示，贵州不同产地的薤白ITS序列长度为534~537bp，其中G+C含量为50.5%~51.1%，平均含量为50.9%，有16个变异位点，包括T-C、T-G、A-G间的转换以及T-A、G-C间的颠换。以单花韭（Allium monanthum Maxim.）为外类群，结合GenBank中下载葱属部分植物序列，基于贝叶斯法和最大简约法构建系统聚类树的结果显示，葱属部分植物可分为4支，其中薤白聚为一支并支持薤白作为一个单系类群，而不同产地的薤白又可分为4小支，其中来自贵阳清镇、六盘水平寨、安顺平坝、黔西南兴仁、铜仁思南的薤白亲缘关系较近，表明基于nrDNA ITS序列能鉴别不同产地薤白的亲缘关系。Abstract: Allium macrostemon Bunge from 18 different habitats in 11 areas of Guizhou Province were taken as study materials. Their nrDNA ITS sequences were compared and analyzed, combined with ITS sequences from Wenchuan in Sichuan， Hanzhong in Shaanxi, China and Korea downloaded from GenBank. Results showed that the lengths of A. macrostemon Bunge sequences from the different areas in Guizhou ranged from 534 bp to 537 bp and the G+C content ranged from 50.5% to 51.1%, with an average content of 50.9% and 16 variable sites, including conversion between T-C, T-G, and A-G. Based on the sequences of A. monanthum Maxim. and other Allium species from GenBank, the plants could be divided into four groups using Bayesian and maximum parsimony methods, with A. macrostemon Bunge polys found on one branch and A. macrostemon Bunge considered to be a monophyletic group. The A. macrostemon Bunge from different areas could be divided into four branches and the phylogenetic relationships of A. macrostemon Bunge from Qingzhen in Guiyang, Pingzhai in Liupanshui, Pingba in Anshun, Xingren in southwest Guizhou, and Sinan in Tongren were found to be very close, indicating that the phylogenetic relationships of A. macrostemon Bunge from different habitats could be identified through nrDNA ITS sequences.
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Keywords:
- Allium macrostemon Bunge /
- ITS sequence /
- Relationship
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