Differentially expressed genes analysis of terpenoid biosynthesis related to aril development in Celastrus orbiculatus Thunb.
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摘要: 为探究南蛇藤（Celastrus orbiculatus Thunb.）假种皮发育过程中萜类物质合成相关基因的表达差异，本文选取3个不同发育时期（盛花期、落花期和幼果期）的南蛇藤雌花为实验材料，构建3个cDNA文库,通过Illumina HiSeqTM 2000平台进行转录组测序，共获得87600个unigene序列，平均长度为453bp，N50/N90比值为4.64。通过3个不同发育阶段基因差异表达的比较及功能富集分析，鉴定出16个萜类合成相关基因，其中8个基因的编码蛋白参与调控7个萜类合成代谢位点。还确定了与萜类合成相关的7个转录因子家族的成员数量以及相对表达量最高的基因。Abstract: To research differentially expressed genes (DEGs) related to terpenoid biosynthesis in the developmental process of aril in Celastrus orbiculatus Thunb., three cDNA libraries from three stages (full blooming, flower senescence, and young fruit) were established and then sequenced by Illumina HiSeqTM 2000. RNA-Seq was assembled into 87 600 unigenes with a mean length of 453 bp and N50/N90 of 4.46. By differential expression analysis of pairwise comparisons and functional enrichment, 21 DEGs were confirmed to have highly differential expression related to terpenoid biosynthesis. Among them, eight genes regulated the metabolism of seven terpenoid synthetases. We also identified seven transcription factor families and high expression genes associated with terpenoid synthesis.
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[1] 吴燕, 郭蕴斐, 卢山. 植物的防御性萜类挥发信号分子[J]. 植物生理学报, 2012, 48(4):311-317. Wu Y, Guo YF, Lu S. Plant terpenoid volatiles as defensive signals[J]. Plant Physiology Journal, 2012, 48(4):311-317.
[2] 王凌健, 方欣, 杨长青, 李建戌, 陈晓亚. 植物萜类次生代谢及其调控[J]. 中国科学:生命科学, 2013, 43(12):1030-1046. Wang LJ, Fang X, Yang CQ, Li JR, Chen XY. Biosynthesis and regulation of secondary terpenoid metabolism in plants[J]. Science China:Life Sciences, 2013, 43(12):1030-1046.
[3] 于安民. 基于RNA-Seq的阳春砂果实发育过程中糖和萜类代谢的研究[D]. 广州中医药大学, 2014. Yu AM. Research on the sugar and terpenoid metabolism during the Amomum villosum Lour, fruit development using RNA-Seq[D].Guangzhou:Guangzhou University of Chinese Medicine, 2014. [4] 赵恒伟, 葛锋, 孙颖, 刘迪秋, 陈朝银. 植物萜类物质生物合成的相关转录因子及其应用前景[J]. 中草药, 2012, 43(12):2512-2519. Zhao HW, Ge F, Sun Y, Liu DQ, Chen CY. Transcription factors involved in plant terpenoid biosynthesis and their application prospect[J]. Chinese Traditional and Herbal Drugs, 2012, 43(12):2512-2519.
[5] Xu YH, Wang JW, Wang S, Wang JY, Chen XY. Characterizationof GaWRKY1, a Cotton transcription factor that regulates the sesquiterpene synthase Gene(+)-δ-Cadinene Synthase-A[J]. Plant Physiol, 2004, 135(1):507-515.
[6] Lu X, Zhang L, Zhang FY, Jiang WM, Shen Q, Zhang LD, Lü ZY, Wang GF, Tang KX. AaORA, a trichome-specific AP2/ERF transcription factor of Artemisia annua, is a positive regulator in the artemisinin biosynthetic pathway and in disease resistance to Botrytis cinerea[J]. New Phytol, 2013, 198(4):1191-1202.
[7] 胡铁锋. 南蛇藤(Celastrus orbiculatus Thunb.)胚胎学研究[D]. 哈尔滨:东北林业大学, 2009. Hu TF. Study on embryology of Celastrus orbiculatus Thunb.[D]. Harbin:Northeast Forestry University, 2009.
[8] Simmons MP. Flowering plants:Celastraceae[M]/.
[9] 赵武. 七种植物假种皮与肉质种皮化学成分与功能研究[D]. 北京:北京林业大学, 2015. Zhao W. Study on components and function of the aril and sarcotesta in seven plants[D]. Beijing:Beijing Foresty University, 2015.
[10] 沐先运.中国南蛇藤属(卫矛科)的分类修订[D].北京:北京林业大学, 2012. Mu XY. Taxonomic revision of Celastrus L.(Celastra-ceae)in China[D]. Beijing:Beijing Foresty University, 2012. [11] Park HJ, Cha DS, Jeon H. Antinociceptive and hypnotic properties of Celastrus orbiculatus[J]. J Ethnopharmacol, 2011, 137(3):1240-1244.
[12] Wu J, Zhou Y, Wang L, Zuo J, Zhao W. Terpenoids from root bark of Celastrus orbiculatus[J]. Photochem, 2012, 75:159-168.
[13] 赵同欣, 刘林德, 张莉, 王艳杰, 贾兴军.南蛇藤花粉和柱头的生物学特性研究[J].植物研究, 2014, 34(3):309-316. Zhao TX, Liu LD, Zhang L, Wang YJ, Jia XJ. Bionomics of pollen and stigma of Celastrus orbiculatus Thunb.[J]. Plant research, 2014, 34(3):309-316.
[14] Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng QD, Chen ZH, Mauceli E, Hacohen N, Gnirke A, Rhind N, Palma FD, Birren BW, Nusbaum C, Toh KL, Friedman N, Regev A. Full length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nat Biotech, 2011, 29(7):644-652.
[15] Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, Baren MJ, Salzberg SL, Barbara J, Wold BJ, Pachter L. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation[J]. Nat Biotech, 2010, 28(5):511-515.
[16] 李建霞. 杨柳科和卫矛科种子附属物的发生、发育研究[D]. 北京:北京林业大学, 2013. Li JX. Morphogenesis and development on seed appen-dages in Salicaceae and Celastraceae[D]. Beijing:Beijing Forestry University, 2013. [17] Mortazavi A, Williams BA, Mccue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nat Methods, 2008, 5(7):621-628.
[18] Bach TJ, Rogers DH, Rudney H. Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings[J]. Eur J Biochem, 1986, 154(1):103-111.
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