Phosphorus deficiency and high light treatment affect the photophysiology and lipid accumulation of Palmellococcus miniatus
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摘要: 以产油微藻集球藻为材料，在低、高两种不同光照强度下（100和600μmol·m-2·s-1），研究缺磷对集球藻光合特性和油脂含量的影响。结果显示，缺磷显著抑制了集球藻的生物量以及光化学效率Fv/Fm，阻碍了QA-向QB的电子传递，降低了细胞内的蛋白含量，诱导了油脂含量增加；高光的耦合作用使集球藻光化学效率Fv/Fm以及QA-向QB电子传递受抑制更严重，细胞内的蛋白含量进一步下降，油脂含量却进一步上升。本研究表明缺磷导致集球藻的代谢从碳同化进入油脂合成，促使油脂含量增加，高光的耦合作用使集球藻的油脂含量进一步增加。Abstract: Palmellococcus miniatus, a lipid-rich green microalgae, was selected as an experimental material. We exposed P. miniatus cultures to low (100 μmol·m-2·s-1) and high (600 μmol·m-2·s-1) light, and investigated the effect of phosphorus deficiency on the photophysiology and lipid accumulation in P. miniatus. Results showed that phosphorus deficiency decreased the photosystem Ⅱ maximum photochemical yield (Fv/Fm), inhibited the electron transfer from QA- to QB, and lowered the protein content, thus resulting in a reduction in growth and biomass. However, phosphorus deficiency induced a significant accumulation of lipids. When supplemented with high light, the photosynthesis of P. miniatus was further suppressed and protein content was markedly decreased, whereas lipid content increased significantly. Our results show that the combined treatments of phosphorus deficiency and high light stimulated lipid accumulation in P. miniatus.
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Keywords:
- Palmellococcus miniatus /
- Phosphorus deficiency /
- High light /
- Photosynthesis /
- Lipid accumulation
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[1] 梅洪, 张成武, 殷大聪, 耿亚红, 欧阳峥嵘, 李夜光. 利用微藻生产可再生能源研究概况[J]. 武汉植物学研究, 2008, 26(6):650-660. Mei H, Zhang CW, Ying DC, Gen YH, OuYang ZR, Li YG. Survey of studies on renewable energy production by microalgea[J]. Journal of Wuhan Botanical Research, 2008, 26(6):650-660. Mei H, Zhang CW, Ying DC, Gen YH, OuYang ZR, Li YG. Survey of studies on renewable energy production by microalgea[J]. Journal of Wuhan Botanical Research, 2008, 26(6):650-660.
[2] Guschina IA, Harwood JL. Lipids and lipid metabolism in eukaryotic algae[J]. Prog Lipid Res, 2006, 45(2):160-186.
[3] Cheng LH, Shen QH, Gong YP, Fang WZ, Bi ZC, Xu XH, Chen HL. Saline wastewater treatment by Chlorella vulgaris with simultaneous algal lipid accumulation triggered by nitrate deficiency[J]. Bioresour Technol, 2015, 193:68-75.
[4] Lee K, Mujtaba G, Choi W, Lee CG. Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions[J].Bioresour Technol, 2012, 123(123):279-283.
[5] Ruiz-Domínguez MC, Vaquero I, Obregón V, Morena B, Vílchez C, Vega J. Lipid accumulation and antioxidant activity in the eukaryotic acidophilic microalga Coccomyxa sp. (strain onubensis) under nutrient starvation[J]. J Appl Phycol, 2015, 27(3):1099-1108.
[6] Wang Y, Liu ZY, Qin S. Effects of iron on fatty acid and astaxanthin accumulation in mixotrophic Chromochloris zofingiensis[J].Biotechnol Lett, 2013, 35(3):351-357.
[7] 刘平怀, 杨勋, 郝宗娣, 王丽波, 黄艳, 朱齐南, 黄斌. 氮、磷源及海盐对微茫藻细胞生长和油脂积累的影响[J]. 食品工业科技, 2013, 34(7):186-189. Liu PH, Yang X, Hao ZD, Wang LB, Huang Y, Zhu QN, Huang B. Effect of nitrogen,phosphrus and sea salt on the cell growth and lipid accumulation of Micractinium reisseri[J]. Science and Technology of Food Industry, 2013, 34(7):186-189.
[8] 靳同霞, 董文静, 郭萌, 代克岩, 杨程, 徐婷婷, 马剑敏. 富营养废水中碳氮磷浓度对布朗葡萄藻总脂和总烃的影响[J]. 植物科学学报, 2012, 30(3):293-298. Jin TX, Dong WJ, Guo M, Dai KY, Yang C, Xu TT, Ma JM. Effects of carbon, nitrogen, phosphorus concentration in eutrophic wastewater on total lipids and total hydrocarbons of Botryococcus braunii[J]. Plant Science Journal, 2012, 30(3):293-298.
[9] Zachleder V, Li XL, Přibyl P, Bišová K, Kwano S, Cepák V. The microalga Parachlorella kessleri-a novel highly efficient lipid producer[J]. Biotechnol Bioeng, 2013, 110(1):97-107.
[10] Reitan KI, Rainuzzo JR, Olsen Y. Effect of nutrient limitation on fatty-acid and lipid-content of marine microalgae[J]. J Phycol, 1994, 30(6):972-979.
[11] Raghothama KG. Phosphate transport and signaling[J]. Curr Opin Plant Biol, 2000, 3(3):182-187.
[12] 石娟, 潘克厚. 不同光照条件对小新月菱形藻和等鞭金藻8701生长及生化成分的影响[J]. 中国水产科学, 2004, 11(2):121-128. Shi J, Pan KH. Effects of different light intensities on growth and biochemical composition of Nitzschia closterium f. minutissima and Isochrysis galbana Parke 8701[J]. Journal of Fishery Sciences of China, 2004, 11(2):121-128.
[13] 李荷芳, 周汉秋. 光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究[J]. 海洋科学集刊, 2001, 43:178-183. Li HF, Zhou HQ. Effect of light intensity on the content of total lipid and fatty acid composition of marine microalgae[J]. Studia Marine Sinica, 2001, 43:178-183.
[14] Li XM, Xia JR. Effects of nitrogen of phosphorus limitation on photosynthetic inorganic carbon utilization and carbo-nic anhydrase activity in Phaeodactylum tricornutum[J]. Acta Hydrobiologica Sinica, 2013, 37(3):405-412.
[19] 施军群, 吴忠兴, 马剑敏,马帅. 水华束丝藻对磷的生理响应研究[J]. 水生生物学报, 2011, 35(5):857-861. Shi JQ, Wu ZX, Ma JM, Ma S. Physiological response to phosphours in Aphanizomenon flosaquae[J]. Acta Hydrobiologica Sinica, 2011, 35(5):857-861.
[20] Jacob J, Lawlor D. In vivo photosynthetic electron transport does not limit photosynthetic capacity in phosphate-deficient sunflower and maize leaves[J]. Plant Cell Environ, 2006, 16(7):785-95.
[21] Rensburg V, Krüger GHJ, Eggenberg P, Strasser RJ. Can screening criteria for drought resistance in Nicotiana tabacum L. be derived from the polyphasic rise of the chlorophyll a fluorescence transient (OJIP)?[J]. S Afr J Bot, 1996, 62(6):337-341.
[22] Krüger GHJ, TsimilliMichael M, Strasser RJ. Light stress provokes plastic and elastic modifications in structure and function of photosystemⅡ in camellia leaves[J]. Physiol Plant, 1997, 101(2):265-277.
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