Cloning and expression analysis of the FaesAP2 gene from Fagopyrum esculentum(Polygonaceae)
-
摘要: 本研究采用同源克隆和RACE技术，从甜荞（Fagopyrum esculentum Moench.）品种'西农9976'中分离出调控花器官发育的FaesAP2基因，该基因序列全长1668 bp，包含1个长为1374 bp的完整开放阅读框，共编码457个氨基酸。序列比对以及系统发育分析结果发现，FaesAP2蛋白拥有2个高度保守的AP2（APETALA2）结构域，在第1个AP2结构域前端有1段由10个氨基酸残基组成的高度保守的核定位信号区；系统发育分析显示其与拟南芥（Arabidopsis thaliana（L.） Heynh.） AP2转录因子的亲缘关系较近。基因表达模式分析表明，该基因在甜荞pin型和thrum型花的雄蕊和雌蕊中有明显的表达，但在幼叶和花被片中不表达，且其表达量在2种类型花不同发育时期呈现明显的变化，均在花药迅速膨大期达到最高值，因此推测该基因在甜荞花发育过程中可能参与了花器官发育的调控。Abstract: The FaesAG gene,which regulates floral development,was isolated from common buckwheat (Fagopyrum esculentum Moench.) ‘xinong9976’ using homologous cloning and RACE methods.Results showed that the FaesAG cDNA was 1668 bp long with a 132 bp 5'leader region,162 bp 3'untranslated region with a poly-A tail,and 1374 bp ORF encoding 457 amino acids.Sequence alignment and conceptual translation revealed that FaesAP2 contained two highly conserved AP2 domains and a putative nuclear localization signal in front of the first AP2 domain.Phylogenetic analyses demonstrated that FaesAP2 is close to the AP2(APETALA2) transcript factor from Arabidopsis thaliana(L.) Heynh.Expression analysis showed that FaesAP2 was mainly expressed in the developing stamen and gynoecium of the pin and thrum flowers,respectively,but was absent from the developing leaves and tepals.Moreover,FaesAP2 expression persisted throughout all stages of pin and thrum flower bud differentiation.When the anthers rapidly increased in size,FaesAP2 expression reached the highest level in the pin and thrum flowers.These results show that FaesAP2 might be involved in regulating floral organogenesis during Fagopyrum esculentum flower development.
-
Keywords:
- Fagopyrum esculentum Moench. /
- Flower development /
- APETALA2 /
- FaesAP2
-
-
[1] Fang ZW, Qi R, Li XF, Liu ZX. Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis AP3 mutant[J]. Gene, 2014, 550(2):200-206.
[2] 孙伟, 安超, 郑希龙, 万和文, 陈延松等. 基于核糖体DNA内转录间隔区ITS序列的广义蓼属及近缘属分子系统学研究[J]. 植物科学学报, 2014, 32(3):228-235. Sun W, An X, Zheng XL, Wan HW, Chen YS, et al. Phylogenetic analysis of Polygonum L. s. lat. and related genera (Polygonaceae) inferred from nrDNA internal transcribed spacer (ITS) sequences[J]. Plant Science Journal, 2014, 32(3):228-235.
[3] 潘凡, 石桃雄, 陈其皎, 孟子烨, 梁成刚, 陈庆富. 苦荞种质主要农艺性状的变异及其对单株粒重的贡献研究[J]. 植物科学学报, 2015, 33(6):829-839. Pan S, Shi TX, Chen QJ, Meng ZY, Liang CG, Chen QF. Variation in major agronomic traits and its contribution to grain weight per plant in tartary buckwheat germplasm[J]. Plant Science Journal, 2015, 33(6):829-839.
[4] Zeng L, Yin Y, You C, Pan Q, Xu D, et al. Evolution and protein interactions of AP2 proteins in Brassicaceae:evidence linking development and environmental responses[J]. Integr Plant Biol, 2016, 58(6):549-563.
[5] Zumajo-Cardona C, Pabón-Mora N. Evolution of the APETALA2 gene lineage in seed plants[J]. Mol Biol Evol, 2016, 33(7):1818-1832.
[6] Krogan NT, Hogan K, Long JA. APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19[J]. Development, 2012, 139(22):4180-4190.
[7] Yan X, Zhang L, Chen B, Xiong Z, Chen C, et al. Functional identification and characterization of the Brassica napus transcription factor gene BnAP2, the ortholog of Arabidopsis thaliana APETALA2[J]. PLoS One, 2012, 7(3):e33890.
[8] Maes T, Van de Steene N, Zethof J, Karimi M, D'Hauw M, et al. Petunia Ap2-like genes and their role in flower and seed development[J]. Plant Cell, 2001, 13(2):229-244.
[9] Karlova R, Rosin FM, Busscher-Lange J, Parapunova V, Do PT, et al. Transcriptome and metabolite profiling show that APETALA2a is a major regulator of tomato fruit ripening[J]. Plant Cell, 2011, 23(3):923-941.
[10] Varkonyi-Gasic E, Lough RH, Moss SM, Wu R, Hellens RP. Kiwifruit floral gene APETALA2 is alternatively spliced and accumulates in aberrant indeterminate flowers in the absence of miR172[J]. Plant Mol Biol, 2012, 78(4-5):417-429.
[11] Houston K, McKim SM, Comadran J, Bonar N, Druka I, et al. Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence[J]. Proc Natl Acad Sci USA. 2013, 110(41):16675-16680.
[12] Salemme M, Sica M, Iazzetti G, Gaudio L, Aceto S. The AP2-like gene OitaAP2 is alternatively spliced and differentially expressed in inflorescence and vegetative tissues of the orchid Orchis italica[J]. PLoS One, 2013, 8(10):e77454.
[13] Luo H, Chen S, Jiang J, Teng N, Chen Y, Chen F. The AP2-like gene NsAP2 from water lily is involved in floral organogenesis and plant height[J]. Plant Physiol, 2012, 169(10):992-998.
[14] Brockington SF, Rudall PJ, Frohlich MW, Oppenheimer DG, Soltis PS, et al. ‘Living stones’ reveal alternative petal identity programs within the core eudicots[J]. Plant J, 2012, 69(2):193-203.
[15] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Mol Biol Evol, 2011, 28(10):2731-2739.
[16] Zeng LP, Zhang Q, Sun RR, Kong HZ, Zhang N, Ma H. Resolution of deep angiosperm phylogeny using conserved nuclear genes and estimates of early divergence times[J]. Nat Commun, 2014, 5:4956.
[17] 刘冰, 叶建飞, 刘夙, 汪远, 杨永等. 中国被子植物科属概览:依据APGⅢ 系统[J]. 生物多样性, 2015, 23(2):225-231. Liu B, Ye JF, Liu S, Wang Y, Yang Y, et al. Families and genera of Chinese angiosperms:a synoptic classification based on APGⅢ [J]. Biodiversity Science, 2015, 23(2):225-231.
[18] Zhang CH, Shangguan LF, Ma RJ, Sun X, Tao R, et al. Genome-wide analysis of the AP2/ERF superfamily in peach (Prunus persica)[J]. Genet Mol Res, 2012, 11(4):4789-4809.
[19] Gil-Humanes J, Pistón F, Martín A, Barro F. Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids[J]. BMC Plant Biol, 2009, 9:66.
[20] Yang FX, Zhu GF, Wang Z, Liu HL, Huang D. A putative miR172-targeted CeAPETALA2-like gene is involved in floral patterning regulation of the orchid Cymbidium ensifolium[J]. Genet Mol Res, 2015, 14(4):12049-61.
[21] Liu Z, Gu C, Chen F, Jiang J, Yang Y, et al. Identification and expression of an APETALA2-like gene from Nelumbo nucifera[J]. Appl Biochem Biotechnol, 2012, 168(2):383-391.
[22] Nilsson L, Carlsbecker A, Sundås-Larsson A, Vahala T. APETALA2-like genes from Picea abies show functional similarities to their Arabidopsis homologues[J]. Planta, 2007, 225(3):589-602.
-
期刊类型引用(3)
1. 谢伟文，马绵英，关开朗，谭广文，梁建，刘世晗. 白云山景区南北坡向次生裸地植被恢复差异分析. 热带农业科学. 2023(08): 93-99 . 
百度学术
2. 王彩玲，景嘉慧，许嘉巍，靳英华，张英洁，陈雪莹. 长白山北坡与西坡岳桦种群差异分析. 植物科学学报. 2022(01): 21-30 . 本站查看
3. 王妙雪，刘琪璟，秦立厚. 基于孢粉记录研究长白山最近两次火山爆发后的植被演替特征. 西北植物学报. 2021(03): 493-500 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 869
- HTML全文浏览量: 2
- PDF下载量: 1073
- 被引次数: 4
下载:
