Physiological effects of nitric oxide on the growth and development of wheat roots under cadmium stress
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摘要: 为了探究外源物一氧化氮（NO）供体硝普钠（sodium nitroprusside，SNP）对Cd2+胁迫下小麦根系生长发育和活性氧代谢的影响，以小麦（Triticum aestivum L.）为材料，研究10 mmol/L CdCl2胁迫下，30 μmol/L硝普钠（含一氧化氮NO）对小麦根系生长发育和活性氧代谢的影响。结果显示，外施SNP后，Cd2+胁迫下的小麦根长度、鲜重与干重较单独镉胁迫处理分别上升了48.0%、107.7%和87.3%，根系超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）的活性分别上升了28.5%、7.4%、19.2%和9.8%，根中超氧自由基（O2.-）和过氧化氢（H2O2）的含量分别降低了80.5％和47.0％；同时外施SNP，使镉胁迫下小麦根中的可溶性糖含量和脯氨酸含量分别上升了24.7％和22.1%；使根中丙二醛（MDA）含量降低了30.2％；使根系活力上升了15.3%。因此，外源NO在一定程度上可以显著提高小麦根的抗氧化能力，增强小麦的抗逆性，缓解镉对小麦根系的毒害，进而促进小麦幼苗根系的生长发育。Abstract: To study the role of sodium nitroprusside (SNP,a donor of nitric oxide (NO)) in alleviating cadmium (Cd) toxicity in wheat (Triticum aestivum L.),wheat seedlings exposed to 10 mmol/L Cd2+ as CdCl2 were treated with 30 μmol/L of SNP.Results showed that Cd2+ exposure depressed plant growth.After SNP application,the length,fresh weight,and dry weight of the roots increased by 48%,107.7%,and 87.3% respectively.In addition,SOD,POD,CAT,and APX increased by 28.5%,7.4%,19.2%,and 9.8%,respectively.The contents of O2.- and H2O2 in the roots decreased by 80.5% and 47.0%,respectively,whereas the contents of soluble sugar and proline increased by 24.7% and 22.1%,respectively.The content of MDA in the roots decreased by 30.2% under Cd2+ stress.Root activity increased by 15.3% under Cd2+ stress after the addition of SNP.Therefore,exogenous SNP significantly improved the antioxidant capacity of wheat roots,which could,in turn,enhance the resistance ability of wheat and alleviate the toxic effects of Cd.Furthermore,SNP could promote the growth and development of wheat seedling roots.
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Keywords:
- Wheat /
- Cadmium stress /
- Nitric oxide /
- Reactive oxygen species metabolism
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