Highly differentiated phylogeographic structure of Primula ranunculoides
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摘要: 毛茛叶报春（Primula ranunculoides Chen）是我国特有的珍稀濒危花卉，其野生种质资源已十分稀少。为了揭示其谱系地理结构，本文对11个居群114个个体的叶绿体trnL-F和trnS-G基因片段进行了测序分析。结果共检测到10个单倍型，除YJPA和YJPB两居群共享一个单倍型（H9）外，其余各居群均仅有一个特有单倍型，居群内不存在单倍型多样性。毛茛叶报春具高分化的谱系地理结构，居群间的总平均遗传距离达0.015，单倍型间的平均突变次数高达31.2步，两者均显著高于同属的近缘种。其种子散布能力弱、生境片断化和地理隔离明显及两年生的生活史可能是导致其高分化谱系地理结构产生的主要原因。Samova、Structure以及TCS分析均支持将毛茛叶报春11个居群的遗传结构分为6组，它们与地形（山脉）关系密切对应，彼此间分化明显，因此建议不同山体的居群应视为不同的保护单元进行遗传管理。Abstract: Primula ranunculoides Chen is a rare and endangered species endemic to China.To reveal its phylogeographic patterns,the trnL-F and trnS-G plastid DNA of 114 individuals sampled from 11 natural populations were sequenced and analyzed.In total,10 haplotypes were identified,and populations were fixed with only one unique haplotype,except for populations YJPA and YJPB,which shared a haplotype (H9).Primula ranunculoides has a highly differentiated and significant phylogeographic structure,with an average genetic distance among populations of 0.015 and average mutation between haplotypes of 31.2 steps,significantly higher than those of related species within the genus.The high differentiation between the populations and haplotypes of this species might be explained by its limited seed dispersal ability,habitat fragmentation,isolation of geographical distance,and biennial life form.Samova,Structure,and TCS analyses unanimously supported the clustering of the 11 sampled populations into six groups,which were closely correlated with topographical features and highly differentiated among each other.Accordingly,populations from different mountains should be considered as different genetic management units for conservation purposes.
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[1] 胡启明, 吴征镒. 中国植物志:第59卷, 第2分册[M]. 北京:科学出版社, 1990:1-277. [2] Richards AJ. Primula[M]. Portland:Timber Press, 2003.
[3] Hao G, Hu CM, Lee NS. Circumscriptions and phylogenetic relationships of Primula Sects. Auganthus and Ranunculoides:evidence from nrDNA ITS sequences[J]. Acta Bot Sin, 2002, 44(1):72-75.
[4] Shao JW, Wu YF, Kan XZ, Liang TJ, Zhang XP. Reappraisal of Primula ranunculoides (Primulaceae), an endangered species endemic to China, based on morphological, molecular genetic and reproductive characters[J]. Bot J Linn Soc, 2012, 169(2):338-349.
[5] 王德元, 彭婕, 陈雅静, 吕国胜, 张小平, 邵剑文. 毛茛叶报春的遗传多样性及遗传结构[J]. 生物多样性, 2013, 21(5):601-609. Wang DY, Peng J, Chen YJ, Lv GS, Zhang XP, Shao JW. Genetic diversity and genetic structure of the rare and endangered species, Primula ranunculoides[J]. Biodiversity Science, 2013, 21(5):601-609.
[6] 陈明林, 张小平, 李晓红. 安徽羽叶报春和毛茛叶报春的微形态特征[J]. 植物资源与环境学报, 2004, 13(3):18-24. Chen ML, Zhang XP, Li XH. Study on micromorphological features of Primula merrilliana and P. cicutariifolia[J]. Journal of Plant Resources and Environment, 2004, 13(3):18-24.
[7] 邵剑文, 张小平, 张中信, 朱国萍. 安徽羽叶报春的有效传粉昆虫及花朵密度和种群大小对传粉效果的影响[J]. 植物分类学报, 2008, 46(4):537-544. Shao JW, Zhang XP, Zhang ZX, Zhu GP. Identification of effective pollinators of Primula merrilliana and effects of flower density and population size on pollination efficiency[J]. Acta Phytotaxonomica Sinica, 2008, 46(4):537-544.
[8] Shao JW, Zhang XP, Zhang ZX, Zhu GP. Effects of population size on reproductive success of the endangered and endemic species Primula merrilliana[J]. Acta Phytotaxon Sin, 2008, 50(9):1151-1160.
[9] Shao JW, Chen WL, Peng YQ, Zhu GP, Zhang XP. Genetic diversity within and among populations of the endangered and endemic species Primula merrilliana in China[J].Biochem Syst Ecol, 2009, 37(6):699-706.
[10] Chen FH. A new Chinese Primula[J]. Not Roy Bot Gard Edin, 1948, 20:120.
[11] Hu CM, Kelso S. Flora of China[M]. Beijing:Science Press, 1996:39-189.
[12] Hewitt GM. Post-glacial recolonization of European biota[J]. Biol J Linn Soc, 1999, 68:87-112.
[13] Manel S, Schwartz MK, Luikart G, Taberlet P. Landscape genetics:combining landscape ecology and population genetics[J]. Trends Ecol Evol, 2003, 18(4):189-197.
[14] Avise JC. Phylogeography:The History and Formation of Species[M]. Cambridge:Harvard University Press, 2000.
[15] Avise JC. Phylogeography:retrospect and prospect[J]. J Biogeogr, 2009, 36(1):3-15.
[16] Petit RJ, Duminil J, Fineschi S, Hampe A, Salvini D, Vendramin GG. Comparative organization of chloroplast, mitochondrial and nuclear diversity in plant populations[J]. Mol Ecol, 2005, 14(3):689.
[17] Liu JQ, Sun YS, GE XJ, Gao LM, Qiu YX. Phylogeographic studies of plants in China:Advances in the past and directions in the future[J]. J Syst Evol, 2012, 50(4):267-275.
[18] Mast AR, Kelso S, Richards AJ, Lang DJ, Feller DMS, Conti E. Phylogenetic relationships in Primula L. and rela-ted genera (Primulaceae) based on noncoding chloroplast DNA[J]. Int J Plant Sci, 2001,162:1381-1400.
[19] Doyle J. DNA Protocols for Plants-CTAB Total DNA Isolation[M]//Hewitt GM, Johnston A, eds. Molecular Techniques in Taxonomy. Berlin:Springer, 1991:283-293.
[20] Taberlet P, Gielly L, Pautou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA[J]. Plant Mol Biol, 1991, 17(5):1105-1109.
[21] Hamilton MB. Four primer pairs for the amplification of chloroplast intergenic regions with intraspecific variation[J]. Mol Ecol, 1999, 8(3):521-523.
[22] Lewis PO, Kumar S, Tamura K, Nei M, Lewis PO. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol, 1994, 10(2):91-189.
[23] Rozas J, Sánchez-Delbarrio JC, Messeguer X, Rozas R. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J].Bioinformatics, 2003, 19(18):2496-2497.
[24] Pons O, Petit RJ. Measuring and testing genetic differentiation with ordered versus unordered alleles[J]. Genetics, 1996, 144(3):1237-1245.
[25] Excoffier L, Lischer HE. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under linux and windows[J]. Mol Ecol Resou, 2010, 10(3):564-567.
[26] Wright S. Isolation by distance under diverse systems of mating[J]. Genetics, 1946, 31(1):39-59.
[27] Dupanloup I, Schneider S, Excoffier L. A simulated annealing approach to define the genetic structure of populations[J]. Mol Ecol, 2002, 11(12):2571-2581.
[28] Pritchard JK, Stephens M, Rosenberg, Peter D. Association mapping in structured populations[J]. Am J Hum Genet, 2000, 67(1):170-181.
[29] Clement M, Posada D, Crandall KA. TCS:a computer program to estimate gene genealogies[J]. Mol Ecol, 2000, 9(10):1657-1659.
[30] 张小平, 陈明林. 安徽羽叶报春与毛茛叶报春的遗传多样性研究与新资源评估[J]. 植物资源与环境学报, 2003, 12(3):1-5. Zhang XP, Chen ML. A study of genetic diversity of Primula merrilliana and Primula cicutariifolia with assessing new ornamental resources[J]. Journal of Plant Resources and Environment, 2003, 12(3):1-5.
[31] Yan HF, Hao G, Hu CM, Ge XJ. DNA barcoding in closely related species:a case study of Primula L. Sect. Proliferae Pax (Primulaceae) in China[J].J Syst Evol, 2011, 49(3):225-236.
[32] Yan HF, Zhang CY, Wang FY, Hu CM, Ge XJ, Hao G. Population expanding with the phalanx model and lineages split by environmental heterogeneity:a case study of Pri-mula obconica in subtropical China[J]. PLoS One, 2012, 7(9):e41315.
[33] Xie XF, Yan HF, Wang FY, Ge XJ, Hu CM, Hao G. Chloroplast DNA phylogeography of Primula ovalifolia in central and adjacent southwestern China:past gradual expansion and geographical isolation[J]. J Syst Evol, 2012, 50(4):284-294.
[34] WangFY, Ge XJ, Gong X, Hu CM, Hao G. Strong genetic differentiation of Primula sikkimensis in the East Himalaya-Hengduan Mountains[J]. Biochem Genet, 2008, 46(1):75-87.
[35] Wang FY, Gong X, Hu CM, Hao G. Phylogeography of an alpine species Primula secundiflora inferred from the chloroplast DNA sequence variation[J]. J Syst Evol, 2008, 46(1):13-22.
[36] 宋敏舒, 乐霁培, 孙航, 李志敏. 横断山地区海仙报春的谱系地理学研究[J]. 植物分类与资源学报, 2011, 33(1):91-100. Song SM, Yue JP, Sun H, Li ZM. Phylogeographical study on Primula poissonii(Primulaceae) from Hengduan Mountains[J]. Plant Diversity and Resources, 2011, 33(1):91-100.
[37] Nybom H, Bartish IV. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants[J]. Perspect Plant Ecol, 2000, 3(2):93-114.
[38] Hewitt GM. Genetic consequences of climatic oscillations in the Quaternary[J]. Philos T R Soc B, 2011, 359(1442):183-195.
[39] 吴征镒. 中国植被[M]. 北京:科学出版社, 1995. [40] Harrison SP, Yu G, Takahara H, Prentice IC. Palaeovegetation:diversity of temperate plants in east Asia[J]. Nature, 2001, 413(6852):129-130.
[41] 胡启明. 报春花科植物的地理分布[J].热带亚热带植物学报, 1994,2(4):1-14. Hu CM. On the geographical distribution of the Primulaceae[J]. Journal of Tropical and Subtropical Botany, 1994, 2(4):1-14.
[42] 王文采. 东亚植物区系的一些分布式样和迁移路线[J]. 植物分类学报, 1992, 30(1):1-24. Wang WC. On some distribution patterns and some migration routes found in the eastern asiatic region[J]. Acta Phytotaxonomica Sinica, 1992, 30(1):1-24.
[43] 王文采. 东亚植物区系的一些分布式样和迁移路线(续)[J]. 植物分类学报, 1992, 30(2):97-117. Wang WC. On some distribution patterns and some migration routes found in the eastern asiatic region(Cont)[J]. Acta Phytotaxonomica Sinica, 1992, 30(2):97-117.
[44] 张小平, 周忠泽. 中国蓼科花粉的系统演化[M]. 合肥:中国科学技术大学出版社, 1998. [45] Hewitt GM. Some genetic consequence of ice ages, and their role in diverging and speciation[J]. Biol J Linn Soc, 1996, 58(3):247-276.
[46] Hewitt G. The genetic legacy of the Quaternary ice ages[J]. Nature, 2000, 405:907-913.
[47] Kelso S. The genus Primula as a model for evolution the Alaskan Flora[J]. Arct Antarct Alp Res, 1992, 24(1):82-87.
[48] Mast AR, Kelso S, Conti E. Are any primroses (Primula) primitively monomorphic[J]. New Phytol, 2006, 171(3):605-616.
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