Effects of light intensity,nitrogen sources,and nitrogen levels on the growth,total lipids,and arachidonic acid accumulation of Parietochloris incisa
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摘要: 对缺刻缘绿藻（Parietochloris incisa（Reisigl） S.Watanabe）在不同光强和氮源及其浓度条件下的生长状况及油脂和花生四烯酸（AA）的积累规律进行了研究。结果显示，缺刻缘绿藻在3种氮源条件下均能较好地生长。在高氮浓度条件下，增大光强能显著提高缺刻缘绿藻的生物量并促进油脂和AA的积累。缺刻缘绿藻在300 μmol·m-2·s-1光强、8.8 mmol·L-1NaNO3条件下生物量达到最大（4.17 g·L-1）。油脂含量在100 μmol·m-2·s-1光强、1.0 mmol·L-1氮浓度下达到最高，分别为41.17%（NaNO3）、42.04%（NH4HCO3）和39.96%（CO（NH2）2）。AA绝对含量在300 μmol·m-2·s-1光强、2.9 mmol·L-1 NaNO3条件下达到最高，占细胞干重的16.44%。油脂和AA产率，在300 μmol·m-2·s-1光强、以NaNO3为氮源的条件下达到最大，分别为134.6 mg·L-1·d-1（1.0 mmol·L-1）和35.85 mg·L-1·d-1（2.9 mmol·L-1）。综合考虑成本等因素，选择NH4HCO3（5.9 mmol·L-1）和CO（NH2）2（2.9 mmol·L-1）为氮源、在300 μmol·m-2·s-1高光强下培养缺刻缘绿藻进行AA的生产为最优方案。Abstract: Parietochloris incisa(Reisigl) S.Watanabe is one of the most promising microorganisms in future health care due to its high arachidonic acid (AA) content.In this study,growth rate and total lipid and AA accumulation in P.incisa were studied under two different light intensities,three different nitrogen sources,and five different nitrogen concentrations.Results showed that three nitrogen sources were utilized by P.incisa.With the increase in light intensity,the growth rate of P.incisa and the contents of total lipids and AA increased significantly under higher nitrogen concentrations.The highest biomass (4.17 g·L-1) was obtained under 300 μmol·m-2·s-1 with 8.8 mmol·L-1 NaNO3 after 14 d of cultivation.For NaNO3,NH4HCO3,and CO (NH2)2 nitrogen sources,the highest total lipid contents were 41.17%(1.0 mmol·L-1),42.04%(1.0 mmol·L-1),and 39.96%(0.5 mmol·L-1),respectively,under the 100 μmol·m-2·s-1 condition.Moreover,the maximum AA content (16.44% DW) of P.incisa was achieved under 300 μmol·m-2·s-1 with 2.9 mmol·L-1 NaNO3.The highest lipid and AA productivities were 134.60 mg·L-1·d-1(1.0 mmol·L-1) and 35.85 mg·L-1·d-1(2.9 mmol·L-1) respectively,under 300 μmol·m-2·s-1 light intensity and NaNO3 nitrogen source condition.Considering the cost factors,NH4HCO3(5.9 mmol·L-1) and CO (NH2)2(2.9 mmol·L-1) as nitrogen sources under 300 μmol·m-2·s-1 high light intensity is recommended for P.incisa cultivation for AA production.
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