Dynamic changes in effective chemical constituents and antioxidant activities in Paeonia lactiflora Pall. during the sun-drying process
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摘要: 为了解芍药根采后干燥过程中有效成分含量和抗氧化能力动态变化，采用高效液相色谱法测定芍药根在晒干过程中芍药苷、芍药内酯苷和苯甲酰基芍药苷的含量，同时测定其甲醇提取液抗氧化能力。结果显示，芍药根干燥过程中芍药苷和苯甲酰基芍药苷的含量均呈先升高后降低的变化趋势，芍药内酯苷的含量呈先下降后升高的变化趋势；晒干初期，芍药根中1，1-二苯基-2-三硝基苯肼（DPPH）自由基清除能力、抑制脂质过氧化能力和超氧阴离子清除能力均显著升高，后期显著降低，但仍然比新鲜样品有显著升高，且均与芍药苷含量呈显著正相关。因此，在干燥初期芍药启动抗干旱胁迫机制，可能通过诱导相关活性成分（如芍药苷）含量升高，增强其抗氧化能力，进而抵御干旱胁迫。Abstract: To investigate the changes in the active ingredients and antioxidant capacity of fresh peony roots in the post-harvest drying process, the contents of paeoniflorin, albiflorin, and benzoylpaeoniflorin in the root during natural drying were determined by high performance liquid chromatography, and antioxidant activities of the materials were detected. Results showed that the content of paeoniflorin and benzoylpaeoniflorin increased at first and then degraded gradually, whereas albiflorin first decreased and then increased. Free radical scavenging ability, including that of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion, as well as inhibition of lipid peroxidation, were significantly increased in the roots during the early phase of the drying process, and there was a significant positive correlation with the content of paeoniflorin. Therefore, the mechanism of resistance to drought stress in the early stage of peony growth might be related to the increase in antioxidant activity and induction of the content of related active ingredients (such as paeoniflorin).
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Keywords:
- Paeonia lactiflora Pall. /
- Sun-dried /
- Chemical constituents /
- Antioxidant
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