基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析

李想; 周亚东; 黄钰倩; 李小燕; 刘星

doi:10.11913/PSJ.2095-0837.2017.60851

基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析

李想¹,
周亚东¹,
黄钰倩¹,
李小燕^2, ,,
刘星^1, ,

1. 武汉大学生命科学学院, 植物系统学与进化生物学实验室, 武汉 430072;
2. 武汉大学生命科学学院, 实验教学中心, 武汉 430072

基金项目:

国家自然科学基金项目（31170203，30870168）。

详细信息

作者简介:
李想(1991-),男,硕士研究生,研究方向为植物系统进化(E-mail:hoscolee@whu.edu.cn)。

通讯作者:
李小燕,E-mail:xiaoyanlixy@sina.com

刘星,E-mail:xingliu@whu.edu.cn

中图分类号: Q943.2
计量
- 文章访问数: 854
- HTML全文浏览量: 1
- PDF下载量: 946
出版历程
- 收稿日期: 2017-05-17
- 网络出版日期: 2022-10-31
- 发布日期: 2017-12-27

Analysis on the genetic diversity and structure of Isoetes taiwanensis based on nuclear and chloroplast DNA sequences

1. Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
2. Experimental Teaching Center, Biology College of Life Sciences, Wuhan University, Wuhan 430072, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31170203, 30870168).

摘要

摘要: 利用核基因和叶绿体基因对台湾水韭（Isoetes taiwanensis Devol）2个居群20个样本的遗传多样性和遗传结构进行了分析，并对台湾水韭的保育提出了建议。核基因的检测结果显示，2个居群共存在18个单倍型，单倍型多样性为0.9842，核苷酸多样性为0.00215，居群间基因流N_m为4.26，居群间分化系数G_st值为0.05543；叶绿体DNA的检测结果显示，单倍型数目为6，单倍型多样性为0.6211，核苷酸多样性为0.00326。其中台北居群的单倍型多样性和核苷酸多样性均比金门居群高。核基因和叶绿体基因的AMOVA分析显示，居群内的遗传差异远高于居群间。台湾水韭的核苷酸多样性相对其他水韭属植物较低，可能与台湾水韭的染色体倍型以及狭域分布有关。居群结构的形成可能和基因流以及奠基者效应有关。宜采用原地和迁地保护的方法对居群进行保护。
- 台湾水韭 /
- 遗传多样性 /
- 遗传结构
Abstract: We employed both nuclear and chloroplast DNA sequences to analyze the genetic diversity and structure of 20 individuals from two separate populations of Isoetes taiwanensis, with some suggestions for the conservation of this species also proposed. In the nuclear DNA data set, 18 haplotypes were discovered, with a haplotype diversity of 0.9842 and nucleotide diversity of 0.00215. In the chloroplast DNA data set, six haplotypes were detected, with a haplotype diversity of 0.6211 and nucleotide diversity of 0.00326. AMOVA results indicated that genetic variations within populations were larger than those among populations. Based on nucleotide DNA sequences, gene flow was 4.26 and G_st was 0.05543. Compared with other Isoetes species, the nucleotide diversity of I. taiwanensis was relatively low, possibly related to the ploidy level and narrow distribution. The genetic structure is likely related to strong gene flow and foundation effects. Both in situ and ex situ conservation is recommended.
- Isoetes taiwanensis /
- Genetic diversity /
- Genetic structure

HTML全文

参考文献(25)

[1]	Troia A, Pereira JB, Kim C, Taylor WC. The genus Isoetes (Isoetaceae):a provisional checklist of the accepted and unresolved taxa[J]. Phytotaxa, 2016, 277(2):101-145.
[2]	于永福. 中国野生植物保护工作的里程碑——《国家重点保护野生植物名录(第一批)》出台[J]. 植物杂志, 1999(5):3.
[3]	Baillie J, Hilton-Taylor C, Stuart SN. 2004 IUCN Red List of Threatened Species:A Global Species Assessment[M]. Cambridge:The IUCN Species Survival Commission, 2004.
[4]	陈进明, 刘星, 王青锋. 中国珍稀濒危特有蕨类植物——云贵水韭的遗传多样性[J]. 武汉大学学报:理学版, 2005, 51(6):767-770. Chen JM, Liu X, Wang QF. Genetic diversity in Isoetes yunguiensis, a rare and endangered endemic fern in China[J]. Journal of Wuhan University:Natural Science Edition, 2005, 51(6):767-770.
[5]	Hamrick JL, Godt MJW, Murawski DA. Correlations between species traits and allozyme diversity implications for conservation biology[M]//Falk DA, Holsinger KE, eds. Genetics and Conservation of Rare Plants. New York:Oxford University Press, 1991.
[6]	吕天锋, 蒿飞, 熊争, 刘星. 中国穗状狐尾藻遗传多样性的初步分析[J]. 植物科学学报, 2016, 34(1):109-116. Lu TF, Hao F, Xiong Z, Liu X. Preliminary analysis on the genetic diversity of Myriophyllum spicatum from China[J]. Plant Science Journal, 2016, 34(1):109-116.
[7]	Leuzinger M, Naciri Y, Pasquier PED, Jeanmonod D. Molecular diversity, phylogeography and genetic relationships of the Silene paradoxa, group of section Siphonomorpha (Caryophyllaceae)[J]. Plant Syst Evol, 2015, 301(1):265-278.
[8]	Kikuchi R, Jae-Hong P, Takahashi H, Maki M. Disjunct distribution of chloroplast DNA haplotypes in the understory perennial Veratrum album ssp. oxysepalum (Melanthiaceae) in Japan as a result of ancient introgression[J]. New Phytol, 2010, 188:879-891.
[9]	Avise JC. Phylogeography:retrospect and prospect[J]. J Biogeogr, 2009, 36:3-15.
[10]	Kim ST, Sultan SE, Donoghue MJ. Allopolyploid speciation in Persicaria (Polygonaceae):Insights from a low-copy nuclear region[J]. Proc Natl Acad Sci USA, 2008, 105:12370-12375.
[11]	Guo YP, Wang SZ, Vogl C, Ehrendorfer F. Nuclear and plastid haplotypes suggest rapid diploid and polyploid speciation in the N Hemisphere Achillea millefolium complex (Asteraceae)[J]. BMC Evol Biol, 2012, 12:2.
[12]	Doyle J. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochem Bull, 1987, 19:11-15.
[13]	Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, et al. Multiple sequence alignment with the clustal series of programs[J]. Nucleic Acids Res, 2003, 31(13):3497-3500.
[14]	Rozas J, Rozas R. DnaSP, DNA sequence polymorphism:an interactive program for estimating population genetics parameters from DNA sequence data[J]. Comput Applic Biosci, 1995, 11(6):621-625.
[15]	Excoffier L, Laval G, Schneider S. Arlequin (version 3.0):an integrated software package for population genetics data analysis[J]. Evol Bioinform, 2007, 1:47-50.
[16]	Chen JM, Liu F, Wang QF. Chloroplast DNA phylogeography of the Chinese endemic alpine quillwort Isoetes hypsophila Hand.-[BFY]Mazz. (Isoetaceae)[J]. Int J Plant Sci, 2008, 169(6):792-798.
[17]	黄钰倩, 李想, 周亚东, 李小燕, 刘星. 基于核基因LEAFY的中国珍稀濒危植物中华水韭的遗传多样性分析[J]. 植物科学学报, 2017, 35(1):73-78. Huang YG, Li X, Zhou YD, Li XY, Liu X. Analysis on the genetic diversity of endangered Isoetes sinensis Palmer from China based on the second intron of LEAFY[J]. Plant Science Journal, 2017, 35(1):73-78.
[18]	陈媛媛, 叶其刚, 李作洲, 黄宏文. 极濒危植物中华水韭休宁居群的遗传结构[J]. 生物多样性, 2004, 12(6):564-571. Chen YY, Ye QG, Li ZZ, Huang HW. Genetic structure of Xiuning population of Isoetes sinensis, endangered species in China[J]. Biodiversity Science, 12(6):564-571.
[19]	Hickey RJ, Taylor WC, Luebke NT. The species concept in Pteridophyta with special reference to Isoetes[J]. Amer Fern J, 1989, 79(2):78.
[20]	Caplen CA, Werth CR. Isozymes of the Isoetes riparia complex,Ⅰ. Genetic variation and relatedness of diploid species[J]. Syst Bot, 2009, 25(2):235.
[21]	Ellstrand NC, Elam DR. Population genetic consequences of small population size:implications for plant conservation[J]. Ann Rev Ecol Syst, 1993,24(24):217-242.
[22]	Templeton AR. Biodiversity at the Genetic Level:Experiences from Disparate Macroorganisms[M]//Hawksworth DL, ed. Biodiversity:Measurement and Estimation. London:Chapman & Hall, 1996:140-141.
[23]	Booy G, Hendriks RJJ, Smulders MJM. Genetic diversity and the survival of populations[J]. Plant Biol, 2000, 2(4):379-395.
[24]	Darvill B, Ellis JS, Lye GC, Goulson D. Population structure and inbreeding in a rare and declining bumblebee, Bombus muscorum (Hymenoptera:Apidae)[J]. Mol Ecol, 2006, 15:601-611.
[25]	杨慧, 陈媛媛, 徐永星, 李作洲. 两种濒危水韭植物迁地保护居群的基因流动态及回归重建保育遗传管理策略[J]. 植物科学学报, 2011, 29(3):319-330. Yang H, Chen YY, Xu YX, Li ZZ. Gene flow dynamics of ex-situ conservation populations in two endangered Isoetes species:genetic implications for reintroduction, conservation and management[J]. Plant Science Journal, 2011, 29(3):319-330.

施引文献(5)

期刊类型引用(4)

1.	李必聪，李慧英，肖遥，罗莎，周庆红，黄英金，朱强龙. 芋扩展蛋白基因家族的全基因组鉴定及其在球茎膨大中的表达分析. 浙江农业学报. 2023(07): 1604-1616 . 百度学术
2.	赵晓宇，苏二虎，王雪娇，刘坤雨，高圆丽，薛春雷，梁红伟，李强. 缺硼对大豆幼苗生长及保护性酶活的影响. 大豆科学. 2023(06): 718-725 . 百度学术
3.	罗萍，王晓萍，张昊楠，范春节，王玉娇，徐建民. 巨桉扩展蛋白EgrEXPA8和EgrEXPA10基因的克隆和表达特性分析. 热带亚热带植物学报. 2023(06): 827-834 . 百度学术
4.	侯佳玉，闫磊，程锦，曾紫君，张雅茹，鲁克嵩，姜存仓. L-天冬氨酸纳米钙促进油菜生长的机理机制. 农业环境科学学报. 2022(07): 1408-1416 . 百度学术