Study on Gentiana crassicaulis Duthie ex Burk. crude extracts and method for breaking dormancy
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摘要: 为了探究影响粗茎秦艽（Gentiana crassicaulis Duthie ex Burk.）种子休眠的因素，破除休眠，寻找其种子快速萌发的方法，以干燥的粗茎秦艽种子为材料，测定种子吸水率及粗提物的活性，使用不同浓度的赤霉素（GA3）、高锰酸钾（KMnO4）、聚乙二醇（PEG6000）和过氧化氢（H2O2）溶液进行浸种处理，比较不同处理条件对粗茎秦艽种子萌发的影响。结果显示，粗茎秦艽种皮对种子吸水没有明显阻碍作用；不同浓度的种子粗提物对白菜、小麦的萌发和生长均表现出抑制作用；不同浓度的粗提物对粗茎秦艽种子自身的萌发也表现出一定的抑制作用，当粗提物浓度达到0.1 g/mL时，抑制作用最显著（P < 0.05）；高锰酸钾处理可提高粗茎秦艽种子的萌发率，浓度为1.5%时效果较显著（P < 0.05），而过氧化氢处理对粗茎秦艽种子的萌发效果不如前者，此外，用500 mg/L的赤霉素浸种和300 mg/L的聚乙二醇预处理也可显著打破粗茎秦艽种子休眠（P < 0.01）。研究结果表明粗茎秦艽种子的内源抑制物是影响其休眠的因素之一；种皮的机械阻碍也在一定程度上影响了种子萌发；粗茎秦艽种子具有综合性休眠特性。高锰酸钾预处理、赤霉素浸种和聚乙二醇引发均可打破种子休眠、缩短种子出芽时间，提高种子的发芽能力。Abstract: We investigated the dormancy mechanism of Gentiana crassicaulis Duthie ex Burk. seeds, as well as the applicable technology to break the seed dormancy and encourage rapid germination. The water absorption of seeds and activity of crude extracts were determined using dried G. macrophylla seeds, which were dry stored for after-ripening. We conducted the germination experiment by soaking seeds in priming solutions of gibberellic acid (GA3), potassium permanganate (KMnO4), polyethylene glycol(PEG6000) or hydrogen peroxide (H2O2), and then compared seed germination under the different treatments. Results showed that the G. crassicaulis seed capsules did not impede water absorption to any great extent. At the different concentrations, the crude extract showed inhibitory effects on the germination and growth of Chinese cabbage and wheat, and a certain inhibition effect on the germination of G. crassicaulis, which was the most significant (P < 0.05) when the concentration reached 0.1 g/mL. The KMnO4 treatment significantly improved the germination rate of G. crassicaulis seeds and was the most significant (P<0.05) at a concentration at 1.5%, whereas treatment with H2O2 had a much less significant effect. In addition, soaking with 500 mg/L of GA3 and priming with 300 mg/L of PEG6000 disturbed the seed dormancy of G. crassicaulis (P < 0.01). Our research showed that the seed endogenous inhibition of G. crassicaulis is one of the factors affecting dormancy, and that the seed mechanical barrier affects seed germination to a certain extent. The G. crassicaulis seeds showed comprehensive dormancy characteristics. Soaking the seeds with KMnO4 and GA3 and priming with PEG6000 interrupted seed dormancy, shortened germination time, and promoted germination.
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Keywords:
- Gentiana crassicaulis Duthie ex Burk. /
- Dormancy /
- Seed /
- Germination
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[1] 郭伟娜, 魏朔南. 秦艽的生物学研究[J]. 中国野生植物资源, 2008, 27(4):1-5, 10. Guo WN, Wei SN. Biological research on Gentiana macrophylla Pall.[J]. Chinese Wild Plant Resources, 2008, 27(4):1-5, 10.
[2] 吴靳荣, 赵志礼, 孟千万. 粗茎秦艽种子生物学研究[J]. 中国中药杂志, 2011, 36(5):552-555. Wu JR, Zhao ZL, Meng QW. A study on the biology of Gentiana crassicaulis Duthie ex Burk. seeds[J]. China Journal Chinese Materia Medica, 2011, 36(5):552-555.
[3] 李建民,李福安,李向阳,张华,魏全嘉,王祖训,弥兰青,肖长宁. 粗茎秦艽不同部位龙胆苦甙含量的分析[J]. 天然产物研究与开发, 2004, 16(3):225-227. Li JM, Li FA, Li XY, Zhang H, Wei QJ, Wang ZX, Mi LQ, Xiao CN. Analysis on the amounts of gentiopicroside in different location of Gentiana crassicaulis Duthie ex Burk.[J]. Natural Product Research and Development, 2004, 16(3):225-227.
[4] 梁晋如,朱砂,张新新,苏琪,何娇,孙文基. 粗茎秦艽的研究概况及展望[J]. 中药材, 2012, 35(3):495-499. Liang JR, Zhu S, Zhang XX, Su Q, He J, Sun WJ. Research progress and prospects of Gentiana crassicaulis Duthie ex Burk.[J]. Journal of Chinese Medicinal Mate-rials, 2012, 35(3):495-499.
[5] 赖晓辉,李群. 种子休眠与萌发分子机制研究进展[J]. 种子, 2014, 33(5):53-58. Lai XH, Li Q. Molecular mechanism of seed dormancy and germination progress[J]. Seed, 2014, 33(5):53-58.
[6] 李康,李丹青,张佳平,夏宜平. 鸢尾属植物种子休眠研究进展[J]. 植物科学学报, 2016, 34(4):662-668. Li K, Li DQ, Zhang JP, Xia YP. Review on seed in Iris[J]. Plant Science Journal, 2016, 34(4):662-668.
[7] 张国华,张艳洁,丛日晨,赵琦,董克奇,古润泽. 赤霉素作用机制研究进展[J]. 西北植物学报, 2009, 29(2):412-419. Zhang GH, Zhang YJ, Cong RC, Zhao Q, Dong KQ, Ku RZ. Advances on gibberellins mechanism[J]. Acta Bota-nica Boreali-Occidentalia Sinica, 2009, 29(2):412-419.
[8] 赵冰, 董进英, 张冬林. 温度、光照和赤霉素浓度对秀雅杜鹃种子萌发的影响[J]. 种子, 2014, 33(5):26-30. Zhao B, Dong JY, Zhang DL. Effects of different temperature, light and GA3 concentration on seed germination of Rhododendron concinnum[J]. Seed, 2014, 33(5):26-30.
[9] 李金还,刘美茹,牛建行,王冉,宋吉轩,吕俊,宗学凤,王三根. 不同处理方式对克氏针茅种子萌发及活力的影响研究[J]. 西南大学学报:自然科学版, 2015, 37(7):67-74. Li JH, Liu MR, Niu JX, Wang R, Song JX, Lv J, Zong XF, Wang SG. Influence of different treatment method on the seed germination and vigor of the Stipa krylovii[J]. Journal Southwest University:Natural Science Edition, 2015, 37(7):67-74.
[10] 刘雪,翟大才,袁涛. 高锰酸钾溶液催芽对樟树发芽率的影响[J]. 种子, 2016, 35(1):101-102. Liu X, Zhai DC, Yuan T. Influence of potassium permanganate sprouting on the Cinnamomum camphora germination rate[J]. Seed, 2016,35(1):101-102.
[11] Heydecker W, Higgins J, Gulliver RL. Accelerated germination by osmotic seed treatment[J]. Nature, 1973, 246(5427):42-44.
[12] 赵玥,辛霞,王宗礼,卢新雄. 种子引发机理研究进展及牧草种子引发研究展望[J]. 中国草地学报, 2012, 34(3):102-108. Zhao Y, Xin X, Wang ZL, Lu XX. Research progress on seed priming mechanization and forage seed priming[J]. Chinese Journal of Grassland, 2012, 34(3):102-108.
[13] 席沁,赵廷宁,张成梁. 若干种子处理方法对偃松种子生活力的影响[J]. 植物科学学报, 2014, 32(4):427-433. Xi Q, Zhao TN, Zhang CL. Influence of treatment on Pinus pumila seed viability[J]. Plant Science Journal, 2014, 32(4):427-433.
[14] 慕小倩,史雷,赵云青,段磊,雷霁. 曼陀罗种子休眠机理与破眠方法研究[J]. 西北植物学报, 2011, 31(4):683-689. Mu XQ, Shi Lei, Zhao YQ, Duan L, Lei J. Seed dormancy mechanism and dormancy breaking method of Datura stramonium L.[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(4):683-689.
[15] 李庆梅,刘艳,刘广全,刘勇,侯龙鱼,胡金鑫. 栎属7种植物种子的发芽抑制物质研究[J]. 生态学报, 2013, 33(7):2104-2112. Li QM, Liu Y, Liu GQ, Liu Y, Hou LY, Hu JX. Germination inhibitory substance extracted from the seed of seven species of Quercus[J]. Acta Ecologica Sinica, 2013, 33(7):2104-2112.
[16] 廖源林,蔡仕珍,李西,林瑞. 野鸦椿种子内源抑制物活性初探[J]. 广西植物, 2016, 36(5):600-606, 538. Liao YL, Cai SZ, Li X, Lin R. Preliminary study of endogenous inhibitors activity of Euscaphis japonica seeds[J]. Guihaia, 2016, 36(5):600-606, 538.
[17] 周艳玲,赵敏,彭元举,赵雨森. 狭叶柴胡种子萌发与内源抑制物质[J]. 植物研究, 2009, 29(3):329-332. Zhou YL, Zhao M, Peng YJ, Zhao YS. Seed germination and endogenous inhibitory substance of Bupleurum scorzoneraefolium[J]. Bulletin of Botanical, 2009, 29(3):329-332.
[18] 常晖,张小燕,张跃进,王渭玲,段琦梅,尤廷. 黄芪种子内源抑制物质的初步研究[J]. 种子, 2015, 34(1):44-47. Chang H, Zhang XY, Zhang YJ, Wang WL, Duan QM, You T. Study on endogenesis inhibitory substances in seed of Astragalus membranaceus[J]. Seed, 2015, 34(1):44-47.
[19] 宋会兴,刘光立,高素萍,陈其兵. 四川牡丹种子浸提液内源抑制物活性初探[J]. 园艺学报, 2012, 39(2):370-374. Song HX, Liu GL, Gao SP, Chen QB. Effects of crude extracts of Paeonia decomposita seeds on germination and activities of antioxidant enzyme of Brassica pekinensis[J]. Acta Horticulturae Sinica, 2012, 39(2):370-374.
[20] 李志丹,韩瑞宏,廖桂兰,张美华. 植物叶片中叶绿素提取方法的比较研究[J]. 广东第二师范学院学报, 2011, 31(3):80-83. Li ZD, Han RH, Liao GL, Zhang MH. The comparative study of the method of plant leaf chlorophyll in the extract[J]. Journal of Guangdong University of Education, 2011, 31(3):80-83.
[21] 王玮. 叶绿素含量的测定[M]//李合生主编. 植物生理生化实验原理和技术. 北京:高等教育出版社,2003:134-137. [22] 周金梅,朱敬.不同药剂浸泡处理对山桃稠李种子发芽的影响[J]. 经济林研究, 2015, 33(1):92-94, 102. Zhou JM, Zhu J. Effects of different agent treatments on germination of Prunus maackii seeds[J]. Nonwood Forest Research, 2015, 33(1):92-94, 102.
[23] 刘丽莎,姬可平. 秦艽种子发芽特性的研究[J]. 中草药, 2002, 33(3):79-81. Liu LS, Ji KP. Germination characters of seed of Gentiana macrophylla Pall.[J]. Chinese Traditional and Herbal Drugs, 2002, 33(3):79-81.
[24] 侯茜,胡锋,张帆,龙凤. 环境因子对濒危药用植物秦艽种子萌发的影响[J]. 中国现代中药, 2016, 18(2):178-180. Hou Q, Hu F, Zhang F, Long F. Effect of environmental factors on endangered medicinal plant Gentiana macrophylla seed germination[J]. Modern Chinese Medicine, 2016, 18(2):178-180.
[25] Debeaujon I, Koornneef M. Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid[J]. Plant Physiol, 2000, 122(2):415-424.
[26] 刘文瑜,魏小红,许可成,赵萌,段正中. 蒺藜苜蓿种子休眠机制及其破除方法研究[J]. 草地学报, 2015, 32(2):358-365. Liu WY, Wei XH, Xu KC, Zhao M, Duan ZZ. Study on dormancy property and breaking approaches of Medicago truncatula seeds[J]. Acta Agrestia Sinica, 2015, 32(2):358-365.
[27] Koen J, Slabbert MM, Bester C, Bierman F. Germination characteristics of dimorphic honeybush (Cyclopia spp.) seed[J]. South African Journal of Botany, 2016, 110(5):68-47.
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