基于MSAP和SSR标记的高粱甲基化连锁群LGC、LGD的构建

段永红; 张旭; 柳青山; 孙毅

doi:10.11913/PSJ.2095-0837.2018.20237

基于MSAP和SSR标记的高粱甲基化连锁群LGC、LGD的构建

段永红^1,2,
张旭¹,
柳青山^1, ,,
孙毅^3,4, ,

1. 山西省农业科学院高粱研究所, 高粱遗传与种质创新山西省重点实验室, 山西晋中 030600;
2. 山西农业大学农学院, 山西太谷 030801;
3. 山西省农业科学院生物技术研究中心, 太原 030031;
4. 农业部黄土高原作物基因资源与种质创制重点实验室, 太原 030031

基金项目:

山西省重点实验室开放课题（2015K-03）；山西省自然科学基金项目（201701D121105）；山西省财政支农项目（2015TGSF-10）。

详细信息

作者简介:
段永红(1974-),女,博士,副教授,研究方向为作物分子育种(E-mail:duanyonghongok@163.com)。

通讯作者:
柳青山,E-mail:673673@126.com

孙毅,E-mail:sunyi692003@163.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2017-08-06
- 网络出版日期: 2022-10-31
- 发布日期: 2018-04-27

Construction of methylation linkage groups LGC and LGD with MSAP and SSR markers in Sorghum bicolor (L.) Moench

1. Sorghum Institute Shanxi Academy of Agricultural Sciences, Key Laboratory of Sorghum Genetic and Germplasm Innovation of Shanxi Province, Jinzhong, Shanxi 030600, China;
2. College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi 030801, China;
3. Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
4. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China

Funds:

This work was supported by grants from the Key Laboratory Innovation of Shanxi Province (2015K-03), Natural Science Foundation of Shanxi Province (201701D121105), and Supporting Agriculture Project from Department of Finance of Shanxi Province (2015TGSF-10).

摘要

摘要: 以高粱（Sorghum bicolor（L.）Moench）品种‘B₂V₄’和‘1383-2’杂交获得的F₂群体为材料，通过SSR和MSAP标记检测高粱基因组差异，构建其甲基化遗传连锁群。结果显示，高粱甲基化连锁群LGC含有3个SSR标记和23个甲基化标记，覆盖高粱基因组44.3 cM；甲基化连锁群LGD含有4个SSR标记和8个甲基化标记，覆盖高粱基因组46.2 cM。LGC上甲基化位点仅来源于EcoRⅠ/MspⅠ酶切组合，而LGD上有来源于EcoRⅠ/MspⅠ和EcoRⅠ/HpaⅡ两种酶切组合的甲基化位点。在LGC连锁群Xtxp 69附近检测到一个密集的甲基化位点区域。研究结果表明MSAP标记可以快速检测植物基因组甲基化差异，适用于构建甲基化连锁群。
- 高粱 /
- SSR /
- MSAP /
- 甲基化
Abstract: The F₂ population derived from crossing sorghum cultivars ‘B₂V₄’ and ‘1383-2’ was used as research material, with the SSR and MSAP markers used to detect the sorghum genome differences and construct methylation linkage groups. Results showed that the methylation linkage group LGC covered 44.3 cM, of which 23 loci were MSAP markers and three loci were SSR markers; the methylation linkage group LGD covered 46.2 cM, of which eight loci were MSAP markers and four loci were SSR markers. The methylation sites on linkage group LGC were from EcoRⅠ/MspⅠ enzyme digestion, but the methylation sites on linkage group LGD were from EcoRⅠ/MspⅠ and EcoRⅠ/HpaⅡ enzyme digestions. A dense methylation region near Xtxp 69 in LGC was identified. The MSAP marker rapidly detected genomic methylation differences and was able to construct a methylation linkage group in plants.
- Sorghum bicolor /
- SSR /
- MSAP /
- DNA methylation

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