不同花期‘西伯利亚’百合花瓣单萜合成途径转录组分析

唐彪; 胡增辉; 冷平生

doi:10.11913/PSJ.2095-0837.2018.20252

不同花期‘西伯利亚’百合花瓣单萜合成途径转录组分析

唐彪¹,
胡增辉^1,2,3, ,,
冷平生^1,2,3, ,

1. 北京农学院园林学院, 北京 102206;
2. 北京林果业生态环境功能提升协同创新中心, 北京 102206;
3. 城乡生态环境北京实验室, 北京 102206

基金项目:

国家自然科学基金项目（31640070，31201645）；北京市属高等学校创新团队建设与教师职业发展计划项目（IDHT20180509）；北京市自然科学基金（6172006）。

详细信息

作者简介:
唐彪(1989-),男,硕士研究生,研究方向为植物生理生态(E-mail:mytobi@163.com)。

通讯作者:
胡增辉,E-mail:buahuzenghui@163.com

冷平生,E-mail:bualengpingsheng@163.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2017-10-18
- 网络出版日期: 2022-10-31
- 发布日期: 2018-04-27

Transcriptome analysis of the monoterpene biosynthesis pathway in petals of Lilium ‘Siberia’ at different flowering stages

Tang Biao¹,
Hu Zeng-Hui^1,2,3, ,,
Leng Ping-Sheng^1,2,3, ,

1. College of Landscape, Beijing University of Agriculture, Beijing 102206, China;
2. Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China;
3. Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 102206, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31640070, 31201645), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20180509), and Natural Science Foundation of Beijing (6172006).

摘要

摘要: 以‘西伯利亚’百合（Lilium ‘Siberia’）花蕾期、半开期、盛开期、衰败期的花瓣为材料，利用RNA-seq技术对其转录组进行高通量测序，分析单萜合成途径中差异表达的基因并阐明其分子机制。结果显示，‘西伯利亚’百合通过转录组测序分析共得到56.28 Gb clean base，223.40 Mb clean reads和124 233个unigene，其中35 749个基因得到注释。萜骨架合成途径中的基因表达水平在不同花期表现出显著差异。其中，甲基赤藓糖醇磷酸（MEP）中的1-脱氧-D-木酮糖-5-磷酸合成酶（DXS）、1-脱氧-D-木酮糖-5-磷酸还原异构酶（DXR）、4-羟基-3-甲丁-2-烯基二磷酸合成酶（HDS）、4-羟基-3-甲丁-2烯基二磷酸还原酶（HDR）、牻牛儿基二磷酸合成酶（GPS）基因的表达水平随花期变化呈先升高后降低的趋势。罗勒烯合成酶（OCS）基因表现出相似变化规律，在盛开期表达量最高。甲羟戊酸（MVA）途径中的3-羟基-3-甲基戊二酸单酰辅酶A还原酶（HMGR）的基因表达同样出现先升高后降低的趋势。单萜合成下游的分支途径中，茄尼基二磷酸合成酶（SDS）、牻牛儿基牻牛儿基二磷酸合成酶（GGDR）基因的表达则出现相反的趋势，在盛开期的表达量最低。研究结果表明MEP途径中的关键基因可随花期变化规律性的表达，以调控单萜的生物合成，在盛开期有较高释放量，且盛开期MVA途径的活化以及泛醌和萜醌代谢支路基因的低表达也促进了单萜的生物合成。
- ‘西伯利亚’百合 /
- 花香 /
- 单萜 /
- 转录组测序 /
- 差异表达基因分析
Abstract: Lilium ‘Siberia’, a typical and fragrant oriental hybrid lily, emits a large amount of monoterpenes, which shows considerable developmental emissions. To date, however, the mechanisms for this remain largely unknown. In this study, we used RNA sequencing (RNA-seq) to determine the petal transcriptome at four different flowering stages, including bud (BS), half-bloom (HS), full-bloom (FS), and late-bloom stages (LS), and analyzed differentially expressed genes(DEGs)to investigate the molecular mechanism of monoterpene biosynthesis. Based on the transcriptome sequencing, we obtained 56.28 Gb of clean bases and 223.40 Mb of clean reads, which were assembled into 124 233 unigenes, 35 749 of which were annotated. The genes in the terpenoid backbone biosynthesis pathway showed significantly different expression at different flowering stages. The gene expression levels of 1-deoxy-D-xylulose 5-phosphate synthase(DXS),1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), and geranyl diphosphate synthase (GPS) first increased and then decreased with flowering stage. The gene expression of ocimene synthase (OCS) exhibited a similar pattern, with a maximum at FS, consistent with monoterpene emission in our previous study. The gene expression of HMG-CoA reductase (HMGR) in the mevalonate (MVA) pathway also presented the same pattern; however, the gene expression patterns of solanesyl-diphosphate synthase (SDS) and geranylgerany1 diphosphate reductase (GGDR) showed the opposite trend and were the lowest during FS in the branched pathway downstream of monoterpene biosynthesis. We demonstrated that the gene expression of key enzymes in the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway regulated the biosynthesis of monoterpenes with flower development, resulting in high release during FS. Moreover, the high activation level of the MVA pathway and the depressed branched metabolic pathway of ubiquinone and other terpenoid-quinones during FS may partly contribute to monoterpene biosynthesis.
- Lilium ‘Siberia’ /
- Floral scent /
- Monoterpene /
- Transcriptome sequencing /
- DEGs analysis

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