钙对盐胁迫下冰叶日中花不同器官离子含量和根部K<sup>+</sup>、Na<sup>+</sup>吸收的影响

李广鲁; 王文果; 陈志新; 胡增辉; 冷平生

doi:10.11913/PSJ.2095-0837.2018.20282

钙对盐胁迫下冰叶日中花不同器官离子含量和根部K⁺、Na⁺吸收的影响

李广鲁¹,
王文果¹,
陈志新¹,
胡增辉^1,2,3, ,,
冷平生^1,2, ,

1. 北京农学院园林学院, 北京 102206;
2. 北京林果业生态环境功能提升协同创新中心, 北京 102206;
3. 城乡生态环境北京实验室, 北京 102206

基金项目:

国家自然科学基金项目（31201645）；北京市属高等学校创新团队建设与教师职业发展计划项目（IDHT20150503，IDHT20180509）。

详细信息

作者简介:
李广鲁(1989-),男,硕士研究生,研究方向为植物生理生态学(E-mail:liguanglu89@126.com)。

通讯作者:
胡增辉,E-mail:buahuzenghui@163.com

冷平生,E-mail:bualengpingsheng@163.com

中图分类号: Q945.78
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出版历程
- 收稿日期: 2017-10-09
- 网络出版日期: 2022-10-31
- 发布日期: 2018-04-27

Effect of calcium on ion contents in different organs and absorption of K⁺ and Na⁺ in the root tips of Mesembryanthemum crystallinum L. under NaCl stress

1. College of Landscape, Beijing University of Agriculture, Beijing 102206, China;
2. Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China;
3. Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 102206, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31201645) and Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20150503, IDHT20180509).

摘要

摘要: 以冰叶日中花（Mesembryanthemum crystallinum L.）实生苗为材料，经NaCl、NaCl+CaCl₂、NaCl+LaCl₃处理后，利用电感耦合等离子发射光谱仪检测叶、茎、根中Na⁺、K⁺、Ca²⁺、Mg²⁺含量，计算K⁺/Na⁺、Ca²⁺/Na⁺和Mg²⁺/Na⁺比值，利用非损伤微测技术测定根尖Na⁺流和K⁺流，研究盐胁迫下钙在维持离子平衡中的作用。结果显示，NaCl处理后，冰叶日中花各器官中Na⁺含量增加，K⁺、Ca²⁺、Mg²⁺含量降低，离子比值降低；CaCl₂处理降低了Na⁺含量，提高了K⁺、Ca²⁺、Mg²⁺含量，离子比值升高，而LaCl₃处理后的结果相反。经NaCl处理24 h后，冰叶日中花根尖Na⁺和K⁺明显外流，加入CaCl₂后，Na⁺外流速度显著增加，K⁺外流速度受到抑制，而加入LaCl₃后则降低了Na⁺的外流速度，促进了K⁺的外流。研究结果表明冰叶日中花受到盐胁迫后，钙参与了促进根部Na⁺外排、抑制K⁺外流的过程，进而保持各器官中较低的Na⁺含量，表明钙在维持和调控离子平衡中起到重要作用。
- 冰叶日中花 /
- 钙 /
- 盐胁迫 /
- 离子含量 /
- 离子平衡
Abstract: In this study, the seedlings of Mesembryanthemum crystallinum L. were used as plant material. After treatment with NaCl, NaCl + CaCl₂, and NaCl + LaCl₃, the contents of Na⁺, K⁺, Ca²⁺, and Mg²⁺ in the leaves, stems, and roots were tested using ICP-OES, with the values of K⁺/Na⁺, Ca²⁺/Na⁺, and Mg²⁺/Na⁺ also calculated to explore the role of calcium in maintaining ion balance under NaCl stress. The fluxes of Na⁺ and K⁺ were also determined by the non-invasive micro-test technique (NMT). Results showed that after NaCl treatment, the content of Na⁺ in the three plant organs increased, whereas the contents of K⁺, Ca²⁺, and Mg²⁺ decreased, as did the ion content ratios. The addition of CaCl₂ reduced the content of Na⁺, but increased the contents of K⁺, Ca²⁺, and Mg²⁺ as well as the ion content ratios. Conversely, LaCl₃ treatment caused the opposite results. After treatment with NaCl for 24 h, significant effluxes of Na⁺ and K⁺ were found in the root tip, indicating that M. crystallinum L. was in a state of ionic imbalance. After the addition of CaCl₂, the efflux rate of Na⁺ increased obviously, whereas that of K⁺ was inhibited. Conversely, the addition of LaCl₃ reduced Na⁺ efflux but accelerated K⁺ efflux. The above results suggested that when M. crystallinum L. exposed to salt stress, calcium promoted Na⁺ efflux but reduced K⁺ efflux in the root tips, and maintained lower Na⁺ content in the three plant organs, suggesting that calcium plays an important role in maintaining and regulating ionic balance.
- Mesembryanthemum crystallinum L. /
- Calcium /
- Salt stress /
- Ion content /
- Ionic balance

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