厚藤<i>ASR</i>基因克隆及功能初步分析

张会; 郑洁旋; 简曙光; 夏快飞; 张美

doi:10.11913/PSJ.2095-0837.2018.30402

厚藤ASR基因克隆及功能初步分析

张会^1,2,
郑洁旋^1,2,
简曙光¹,
夏快飞¹,
张美^1, ,

1. 中国科学院华南植物园, 广东省应用植物学重点实验室, 广州 510650;
2. 中国科学院大学, 北京 100049

基金项目:

国家重点研发计划项目（2016YFC1403002）；中国科学院A类战略性先导科技专项（XDA13020500）；“十二五”农村领域国家科技计划项目（2015BAL04B04）。

详细信息

作者简介:
张会(1989-),女,硕士研究生,研究方向为植物逆境生理(E-mail:1522011738@qq.com)。

通讯作者:
张美,E-mail:zhangmei@scbg.ac.cn

中图分类号: Q943.2
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出版历程
- 收稿日期: 2017-10-23
- 网络出版日期: 2022-10-31
- 发布日期: 2018-06-27

Isolation and functional characterization of the ASR gene from Ipomoea pes-caprae

1. Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Funds:

This work was supported by grants from the National Key Research and Development Program of China (2016YFC1403002), ‘Strategic Priority Research Program’ of the Chinese Academy of Sciences (XDA13020500), and "Twelfth Five-Year" National S & T Projects for Rural Areas (2015BAL04B04).

摘要

摘要: 通过对厚藤（Ipomoea pes-caprae （Linn.）Sweet.）cDNA文库的筛选，获得了一个编码厚藤ASR（ABA-stress-ripening）基因的全长cDNA，命名为IpASR。研究结果显示，IpASR编码区全长648 bp，共编码215个氨基酸；蛋白质等电点为5.42，分子量为24.57 kD。通过在酵母中表达，发现IpASR能够提高转基因酵母的耐盐性及抗氧化能力。进一步以厚藤成年植株及幼苗为材料进行实时荧光定量PCR分析，结果表明，IpASR基因在厚藤成年植株各组织中广泛表达；高盐、甘露醇胁迫和ABA处理可诱导该基因在厚藤幼苗中的表达。结合GFP融合蛋白的亚细胞定位和生物信息学分析，发现IpASR蛋白为核蛋白，推测IpASR基因参与了厚藤生长发育的调控，并可响应ABA和非生物胁迫的诱导。
- 厚藤 /
- ASR基因 /
- 逆境胁迫 /
- 亚细胞定位
Abstract: We focused on the full-length cDNA encoding ASR protein isolated from a Ipomoea pes-caprae (Linn.) Sweet. cDNA library. Results showed the coding region of IpASR cDNA was 648 bp, encoding a 215 amino acid protein with a molecular weight of 24.57 kD and isoelectric point of 5.42. By ectopic expression of IpASR in yeast, we found that IpASR improved the salt and H₂O₂ tolerance of transgenic yeast strains. Using adult plants and seedlings of I. pes-caprae with or without abiotic stress and ABA treatment, real-time RT-PCR analysis showed that IpASR was widely expressed in different adult organs in I. pes-caprae. The IpASR transcript was induced under abiotic stress and ABA treatment. The subcellular localization assay combining bioinformatics analysis showed that IpASR was a nucleoprotein. These results suggest that IpASR might play an important role in the regulation of I. pes-caprae growth and development, and therefore respond to environmental abiotic stress and the ABA signal pathway.
- Ipomoea pes-caprae /
- ASR /
- Abiotic stress /
- Subcellular localization

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