营养盐初始组合浓度对类波氏真眼点藻生长和油脂积累的影响

陈爱玲; 高保燕; 黄罗冬; 王飞飞; 张成武

doi:10.11913/PSJ.2095-0837.2018.30420

营养盐初始组合浓度对类波氏真眼点藻生长和油脂积累的影响

暨南大学生态学系, 水生生物研究中心, 广州 510632

基金项目:

国家高技术研究发展计划“863”项目（2013AA065805）；国家自然科学基金（31170337）；广东省低碳专项（2011-051）；珠海市科技重大项目（PB20041018）；珠海市科技攻关项目（PC20081008）。

详细信息

作者简介:
陈爱玲(1992-),女,硕士研究生,研究方向为微藻生物技术(E-mail:994861146@qq.com)。

通讯作者:
张成武,E-mail:tzhangcw@jnu.edu.cn

中图分类号: Q949.25⁺1
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出版历程
- 收稿日期: 2017-12-10
- 网络出版日期: 2022-10-31
- 发布日期: 2018-06-27

Effects of initial combined concentrations of nutrients on the growth and lipid accumulation of Eustigmatos cf. polyphem

Research Centre for Hydrobiology, Department of Ecology, Jinan University, Guangzhou 510632, China

Funds:

This work was supported by grants from the National High-Tech R & D Program (863 Program) (2013AA065805), National Natural Science Foundation of China (31170337), Guangdong Low-Carbon Program (2011-051), Zhuhai Major Program of Science and Technology (PB20041018), and Key Project of Science and Technology of Zhuhai (PC20081008).

摘要

摘要: 为了研究培养基中主要营养元素氮、磷、硫初始组合浓度对类波氏真眼点藻（Eustigmatos cf. polyphem D. J. Hibberd）生长、油脂积累和脂肪酸组成的影响，分别以NaNO₃、K₂HPO₄、MgSO₄为氮、磷、硫源，设置不同的营养盐初始组合浓度对其进行培养，采用干重法、重量法、气相色谱分析法、元素分析仪等依次对生物量、总脂、脂肪酸组成和细胞内元素含量进行测定。结果显示，氮、磷、硫三者的初始组合浓度变化对类波氏真眼点藻的生长和油脂积累具有明显的影响，9 mmol/L NaNO₃、0.92 mmol/L K₂HPO₄、0.3 mmol/L MgSO₄营养条件下其生物量达到最大值，为9.19 g/L；低氮、低磷胁迫或二者共同胁迫均促进其油脂积累，但低硫胁迫对油脂的积累影响不大，最大油脂含量出现在3 mmol/L NaNO₃、0.029 mmol/L K₂HPO₄、0.3 mmol/L MgSO₄ 条件下，为68.7%（DW）。此外，类波氏真眼点藻富含棕榈油酸，占总脂肪酸含量的最大值达60.88%。选择合理的氮、磷、硫初始组合浓度能够有效促进其生长或油脂及棕榈油酸的积累。
- 类波氏真眼点藻 /
- 氮 /
- 磷 /
- 硫 /
- 总脂 /
- 脂肪酸
Abstract: To determine the effects of different initial combined concentrations of N, P and S on the growth, lipid accumulation, and fatty acid compositions of Eustigmatos cf. polyphem D. J. Hibberd, we cultivated the algae in modified BG-11 medium containing different combined concentrations of nutrients (sodium nitrate, dipotassium phosphate, and magnesium sulfate used as nitrogen (N), phosphorus (P), and sulfur (S) sources, respectively). Biomass concentration, total lipid content, fatty acid composition, and primary intracellular nutrient element content were measured by dry weight, gravimetrics, gas chromatography, and elemental analyzer, respectively. Results showed that different initial combinations of N, P, and S significantly affected the growth, lipid accumulation, and fatty acid composition of E. cf. polyphem. The highest biomass concentration reached 9.19 g/L under 9 mmol/L NaNO₃, 0.92 mmol/L K₂HPO₄, and 0.3 mmol/L MgSO₄. Low N or P stress or combined low N and P stress promoted lipid accumulation, though there was no obvious influence on lipid accumulation under low S stress. The highest total lipid content reached 68.7% of dry weight under 3 mmol/L NaNO₃, 0.029 mmol/L K₂HPO₄, and 0.3 mmol/L MgSO₄. The E.cf.polyphem fatty acids were rich in palmitoleic acid, with relative amounts reaching 60.88% of total fatty acid. Thus, reasonable combined concentrations of N, P, and S effectively promoted growth and lipid and palmitoleic acid accumulation of E. cf. polyphem.
- Eustigmatos cf. polyphem /
- Nitrogen /
- Phosphorus /
- Sulfur /
- Total lipid /
- Fatty acids

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参考文献(8)

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