基于核基因分子多态性的葡萄属物种鉴定

张天缘; 王白石; 宋驰; 张燕君; 徐小玉; 杨雪莹; 张瑾; 张颖; 刘开会; 彭笑天; 裴黎

doi:10.11913/PSJ.2095-0837.2018.40569

基于核基因分子多态性的葡萄属物种鉴定

1. 中国中医科学院中药研究所, 北京 100700;
2. 公安部物证鉴定中心, 北京 100038;
3. 中国科学院武汉植物园, 武汉 430074

基金项目:

中央级公益性科学院所基本科研业务费专项资金（2015JB001）。

详细信息

作者简介:
张天缘(1990-),男,硕士研究生,研究方向为药用植物基因组学(E-mail:zhangtianyuan@foxmail.com);王白石(1984-),男,博士,研究方向为植物基因组学(E-mail:wangbaishi1018@163.com)。

通讯作者:
裴黎,E-mail:pl128@126.com

中图分类号: Q943.2;S663.1
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出版历程
- 收稿日期: 2018-01-03
- 网络出版日期: 2022-10-31
- 发布日期: 2018-08-27

Identification of Vitis species based on nuclear gene molecular polymorphism

1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
2. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China;
3. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

Funds:

This work was supported by a grant from the Special Fund for Basic Scientific Research Business of Central Public Research Institutes (2015JB001).

摘要

摘要: 葡萄属（Vitis）植物主要分布于东亚、北美洲以及欧洲至中亚地区。由于葡萄主要采用无性繁殖方式，地区间品种交流较多，同名异物及同物异名现象较为普遍，给葡萄属物种的分类、鉴定造成一定困难。本研究通过序列同源性比对的方法对葡萄属4种植物基因组的保守编码区进行比对，挖掘保守序列93条，经过验证，找到了一条可用于开发多态性分子标记的KOG3174同源序列，并根据该序列设计得到3对引物，可用于4种葡萄的分类、鉴定。
- 葡萄属 /
- 单核苷酸多态性 /
- 保守序列
Abstract: Vitaceae are a family of dicotyledons that contain 16 genera and more than 700 species. Among them, Vitis is a typical genus mainly distributed in East Asia, North America, Europe, and Central Asia. Grapes mainly propagate via asexual reproduction and have many inter-regional exchange events among varieties, which lead to homonym or synonym phenomena and difficulties in classification and identification. In this study, we compared the conserved coding regions of four species of Vitis and developed 93 conserved sequences. We found that a sequence homologous to KOG3174 could be used to develop polymorphic molecular markers. We designed three pairs of primers, which could be implemented for the identification of the four species of Vitis at the molecular level.
- Vitis /
- Single nucleotide polymorphisms (SNP) /
- Conserved sequence

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参考文献(29)

[1]	唐云涛, 粟桂蓉, 谢毓, 易浪波. 基于PsbA-trnH, RbcL和trnL-F序列的野生葡萄系统发育[J]. 吉首大学学报:自然科学版, 2014, 35(2):85-89. Tang YT, Su GR, Xie Y, Yi LB. Phylogenetic analysis of three wild grapes based on PsbA-trnH, RbcL and trnL-F sequences[J]. Journal of Jishou University:Natural Science Edition, 2014, 35(2):85-89.
[2]	王发松, 朱长山. 中国葡萄属药用植物资源调查研究[J]. 中药材, 2000, 23(6):317-319. Wang FS, Zhu CS. Investigation of medicinal plant resources of Vitis in China[J]. Journal of Chinese medicinal materials, 2000, 23(6):317-319.
[3]	李贝贝. 葡萄DNA指纹数据库的构建及品种鉴定[D]. 洛阳:河南科技大学, 2017.
[4]	段来军. 基于叶片形态变异探讨中国葡萄属分类问题[D]. 武汉:华中农业大学, 2016.
[5]	王娟, 陶永焕, 宋尚伟. 葡萄EST-SSR引物的开发及部分种质聚类分析[J]. 华北农学报, 2014, 29(2):121-126. Wang J, Tao YH, Song SW. Development of EST-SSR primers and cluster analysis of some cultivars in grape[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(2):121-126.
[6]	Délye C, Laigret F, Corio-Costet M. New tools for studying epidemiology and resistance of grape powdery mildew to DMI fungicides[J]. Pest Manag Sci, 2015, 51(3):309-314.
[7]	Teh SL, Fresnedo-Ramírez J, Clark MD, Gadoury DM, Sun Q, et al. Genetic dissection of powdery mildew resis-tance in interspecific half-sib grapevine families using SNP-based maps[J]. Mol Breed, 2017, 37(1):1.
[8]	Frotscher J, Nocentini M, Ruehl E, Bitz O. Quality control:identification of table grape cultivars using RAPD markers[J]. Acta Hort, 2014, 1048:193-196.
[9]	金炳奎, 宗成文, 曹后男, 朴日子, 许雪. 山葡萄SRAP技术体系的建立及其在品种鉴定中的应用[J].吉林农业大学学报, 2013, 35(2):198-205. Jin BK, Zong CW, Cao HN, Piao RZ, Xu X. Establshment of SRAP-PCR system and variety identification on Vtis amurensis[J]. Journal of Jilin Agricultural University, 2013, 35(2):198-205.
[10]	Li ZQ, Li TM, Wang YJ, Xu Y. Breeding new seedless grapes using in ovulo, embryo rescue and marker-assisted selection[J]. In Vitro Cell Dev Biol, 2015, 51(3):241-248.
[11]	Li ZT, Dhekney SA, Gray DJ. Molecular characterization of a SCAR marker purportedly linked to seedlessness in grapevine (Vitis)[J]. Mol Breed, 2010, 25(4):637-644.
[12]	李继洋, 代培红, 罗淑萍. 基于ISSR的葡萄品种亲缘关系分析及其指纹图谱构建[J]. 新疆农业大学学报, 2014, 37(1):24-29. Li JY, Dai PH, Luo SP. Analysis of genetic relationship and establishment of DNA fingerprinting for grape varieties by ISSR markers[J]. Journal of Xinjiang Agricultural University, 2014, 37(1):24-29.
[13]	李贝贝, 刘崇怀, 姜建福, 张颖, 樊秀彩, 张国海. 葡萄品种分子鉴定研究进展及展望[J]. 江苏农业科学, 2017, 45(15):15-20. Li BB, Liu CH, Jiang JF, Zhang Y, Fan XC, Zhang GH. Progress and prospect on molecular identification of grape variety[J]. Jiangsu Agricultural Sciences, 2017, 45(15):15-20.
[14]	王姣, 刘崇怀, 樊秀彩, 孙海生. 葡萄种类和品种鉴定技术研究进展[J]. 植物遗传资源学报, 2008, 9(3):401-405. Wang J, Liu CH, Fan XC, Sun HS. Progress on identification technique of grape species and cultivars[J]. Journal of Plant Genetic Resources, 2008, 9(3):401-405.
[15]	Isenbarger TA, Carr CE, Johnson SS, Finney M, Church GM, et al. The most conserved genome segments for life detection on earth and other planets[J]. Orig Life Evol Biosph, 2008, 38(6):517-533.
[16]	Edwards D. Plant Bioinformatics[M]. New Jersey:Humana Press, 2007.
[17]	涂四利, 方伟武. 脊椎动物线粒体全基因组中的超级保守序列与进化[J].科学通报, 2006, 51(4):427-430. Tu SL, Fang WW. The superconserved sequence and evolution of vertebrate mitochondrial whole genomes[J]. Chinese Science Bulletin, 2006, 51(4):427-430.
[18]	姜运良, 刘浩, 孙亿, 刘根, 鹿宏宇, 等. 一种筛选抗猪圆环病毒病猪的SERPINA1分子标记育种方法及其应用:CN105861704A[P]. 2016-08-17.
[19]	蔡海亚, 徐得泽, 游艾青, 周雷, 焦春海. 水稻抗稻瘟病基因Pita特异性分子标记引物及应用:CN105907884A[P]. 2016-08-31.
[20]	Parra G, Bradnam K, Korf I. CEGMA:a pipeline to accurately annotate core genes in eukaryotic genomes[J]. Bioinformatics, 2007, 23(9):1061-1067.
[21]	周喜旺, 刘鸿燕, 王娜, 南海, 赵尚文, 等. DNA分子标记技术在小麦遗传育种中的应用综述[J]. 甘肃农业科技, 2017, (5):64-68. Zhou XW, Liu HY, Wang N, Nan H, Zhao SW, et al. Application review of DNA molecular markers technique in wheat genetic breeding[J]. Gansu Agricultural Science and Technology, 2017, (5):64-68.
[22]	Mihaljević MŽ, Anhalt UCM, Rühl E, Mugoša MT, Maraš V, et al. Cultivar identity, intravarietal variation, and health status of native grapevine varieties in Croatia and Montenegro[J]. Am J Enol Viticult, 2015, 66(4):531-541.
[23]	Huber F, Rockel F, Schwander F, Maul E, Eibach R, et al. A view into American grapevine history:Vitis vinifera cv. ‘Semillon’ is an ancestor of ‘Catawba’ and ‘Concord’[J]. Vitis, 2016, 55(2):53-56.
[24]	马晓冲, 姚辉, 辛天怡, 陈晓辰, 宋经元. 基于DNA条形码SNP鉴别东方泽泻、泽泻及市售泽泻药材[J]. 中国药学杂志, 2015, 50(17):1474-1478. Ma XC, Yao H, Xin TY, Chen XC, Song JY. SNP identification of Alisma orientale, Alisma plantago-aquatica and Alismatis rhizoma in the market based on DNA barcoding[J]. China Journal of Chinese Materia Medica, 2015, 50(17):1474-1478.
[25]	李小白, 向林, 罗洁, 胡标林, 田胜平, 等. 转录组测序(RNA-seq)策略及其数据在分子标记开发上的应用[J]. 中国细胞生物学学报, 2013, 35(5):720-726, 740. Li XB, Xiang L, Luo J, Hu BL, Tian SP, et al. The strategy of RNA-seq, application and development of molecular marker derived from RNA-seq[J]. Chinese Journal of Cell Biology, 2013, 35(5):720-726, 740.
[26]	李尊严, 范垂姝, 郭海洋, 郑亮亮, 李思瑶, 孙世龙. 中国汉族人群中EED基因多态性与肝细胞癌易感性的关联性研究[J]. 中国地方病防治杂志, 2017, 32(3):1-5. Li ZY, Fan CS, Guo HY, Zheng LL, Li SY, Sun SL. Correlation between EED gene polymorphism and susceptibility to hepatocellular carcinoma in Chinese Han population[J]. Chinese Journal of Control of Endemic Disenaces, 2017, 32(3):1-5.
[27]	姚丹青, 楼坚锋, 朱文莹, 张微微, 夏建明. 基于SNP标记的黄瓜遗传多样性分析[J]. 上海农业学报, 2017, 33(1):21-30. Yao DQ, Lou JF, Zhu WY, Zhang WW, Xia JM. Genetic diversity analysis of cucumber based on SNP markers[J]. Acta Agriculturae Shanghai, 2017, 33(1):21-30.
[28]	Hendry LM, Wadley AL, Cherry CL, Price P, Lombard Z, Kamerman PR. TNF block gene variants associated with pain intensity in black southern Africans with HIV-associated sensory neuropathy[J]. Clin J Pai, 2016, 32(1):45-50.
[29]	Fondevila M, Børsting C, Phillips C, de la Puente M, Santos C, et al. Forensic SNP genotyping with SNaPshot:Technical considerations for the development and optimization of multiplexed SNP assays[J]. Forensic Sci Rev, 2017, 29(1):57-76.

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