Cloning and preliminary functional analysis of AmNAC6 from Ammopiptanthus mongolicus
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摘要: 以强抗逆植物沙冬青（Ammopiptanthus mongolicus（Maxim.ex Kom.）Cheng f.）为材料，克隆获得一个NAC转录因子基因AmNAC6的全长cDNA，并对其序列特征、蛋白质亚细胞定位和表达模式进行了分析。研究结果表明，AmNAC6基因的编码蛋白由304个氨基酸组成，具有NAC家族典型的结构特征，亚细胞定位实验证实该蛋白分布于细胞核内。表达图谱分析结果显示，在室内培养的沙冬青幼苗中，AmNAC6的转录水平受干旱、高盐、低温和高温胁迫的影响，其中在干旱诱导下该基因的转录水平上调较为明显；野外生长植株的嫩叶中，该基因的转录水平在中秋和初冬略低于其他季节，春季则低于其在侧根、嫩枝、花蕾和未成熟果荚中的转录水平。此外，本研究还将AmNAC6 基因成功地构建到植物表达载体。Abstract: The full-length cDNA of a NAC transcription factor gene (AmNAC6) was cloned from Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f., a plant with high tolerance to abiotic stresses, with the sequence characteristics, protein subcellular localization, and expression patterns of AmNAC6 then analyzed. Results showed that AmNAC6 encoded a 304 amino acid residue protein, which exhibited typical structural features of the NAC transcription factor family. Subcellular localization confirmed the nuclear distribution of the AmNAC6 protein. Expression analysis demonstrated that the transcription level of AmNAC6 in laboratory-cultured A. mongolicus seedlings changed during drought, salt, cold, and heat stress treatments, with the drought-induced expression more obvious than that under the other three treatments. In the young leaves of wild plants, the transcription levels were slightly lower in mid-autumn and early winter than in other seasons. The transcription level of the gene in young leaves was obviously lower than that in lateral roots, young branches, flower buds, and immature pods in spring. Moreover, the plant expression vector of AmNAC6 was successfully constructed, laying a foundation for further functional analyses.
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