Effects of three plant exogenous hormones on the biomass, chlorophyll fluorescence parameters, and photosynthetic characteristics of Tabebuia chrysantha seedlings
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摘要: 以黄花风铃木（Tabebuia chrysantha（Jacq.）Nichols）幼苗为材料，采用L16（45）正交实验方法，测定生根粉（GGR）、3-吲哚乙酸（IAA）、多效唑（PP333）3种外源植物激素不同组合处理下黄花风铃木幼苗的生物量、叶绿素含量、叶绿素荧光参数以及光合特性，研究3种外源植物激素不同组合对黄花风铃木幼苗生长和光合作用的影响，筛选出适宜黄花风铃木幼苗生长的最佳浓度组合。结果显示：11号处理（GGR 400 mg/L、IAA 400 mg/L、PP333 400 mg/L）幼苗鲜重、干重均大于其他组合处理。3种外源植物激素中，GGR对幼苗鲜重、幼苗干重增长起主导作用，IAA和PP333的作用不明显。11号处理下的幼苗净光合速率、气孔导度、蒸腾速率、叶绿素含量、叶绿素荧光参数均大于其他处理，胞间二氧化碳浓度值最低。分析得出IAA对黄花风铃木幼苗的净光合速率、气孔导度、蒸腾速率、光合系统潜在活性、最大光能转换效率和叶绿素含量起主导作用，GGR和PP333的作用不明显。综合分析各指标得出，适宜黄花风铃木幼苗生长的最佳外源激素浓度组合为11号处理，此浓度下黄花风铃木幼苗综合质量最佳。Abstract: We applied a L16(45) orthogonal experimental design to determine and compare the biomass, chlorophyll content, chlorophyll fluorescence parameters, and photosynthetic characteristics of Tabebuia chrysantha (Jacq.) Nichols seedlings under different combinations of rooting powder (GGR), indole acetic acid (IAA), and paclobutrazol (PP333). The effects of the different combinations of plant exogenous hormones (GGR, IAA, and PP333) on the growth and photosynthesis of T. chrysantha seedlings were used to derive the optimal concentration combination. Results showed that fresh weight and dry weight were highest under Treatment 11 (GGR 400 mg/L, IAA 400 mg/L, PP333 400 mg/L) compared with the other treatments. Among the three plant exogenous hormones, GGR played a leading role in the increase in fresh and dry weights, whereas IAA and PP333 did not have a significant effect. The net photosynthetic rate, stomatal conductance, transpiration rate, chlorophyll content, and chlorophyll fluorescence parameters of seedlings were also highest under Treatment 11, whereas intracellular carbon dioxide concentration was lowest. Results also showed that IAA played a leading role in the net photosynthetic rate, stomatal conductance, transpiration rate, potential activity of photosynthesis system, maximum light energy conversion efficiency, and chlorophyll content of T. chrysantha seedlings, whereas the effects of GGR and PP333 were not obvious. Comprehensive analysis further showed that the appropriate concentration combination of different exogenous hormones was that of Treatment 11, under which the quality of T. chrysantha seedlings was optimal.
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[1] 王熙. 园林树种黄花风铃木[J]. 广东林业科技, 2007, 23(2):115-116. Wang X. Botanical garden tree species of Tabebuia chrysantha[J]. Guangdong Forestry Science and Technology, 2007, 23(2):115-116.
[2] 何英姿. 氮磷钾平衡施肥对黄花风铃木幼苗生长生理的影响[D].南宁:广西大学, 2016. [3] 林文胜. 黄花风铃木的栽培技术研究及绿化应用[J]. 中国园艺文摘, 2011, 27(12):117-118. Lin WS. Cultivation techniques and application of Tabebula chrysantha[J]. Chinese Horticultural Digest, 2011, 27(12):117-118.
[4] 钟海智, 林耿, 陈志刚, 邹永灵, 彭海新. 黄花风铃木种苗培育技术[J]. 广东林业科技, 2015, 31(2):146-148. Zhong HZ, Lin G, Chen ZG, Zou YL, Peng HX. Cultivation technology of Tabebula chrysantha seedlings[J]. Guangdong Forestry Science and Technology, 2015, 31(2):146-148.
[5] 尚秀华, 高丽琼, 张沛健, 林彦, 彭彦. 3种风铃木扦插繁殖技术研究[J]. 桉树科技, 2016, 33(1):38-42. Sang XH, Gao LQ, Zhang PJ, Lin Y, Peng Y. Three kinds of Tabebula chrysantha cuttings[J]. Eucalypt Science & Technology, 2016, 33(1):38-42.
[6] 张锋, 潘康标, 田子华. 植物生长调节剂研究进展及应用对策[J]. 现代农业科技, 2012(1):193-195. Zhang F, Pan KB, Tian ZH. Research progress and application countermeasures of plant growth regulators[J]. Modern Agricultural Sciences and Technology, 2012(1):193-195.
[7] 陶奇波, 白梦杰, 韩云华, 冯葆昌, 王彦荣. 植物生长调节剂在牧草种子生产中的应用[J]. 草业科学, 2017, 34(6):1238-1246. Tao QB, Bai MJ, Han YH, Feng BC, Wang YR. Use of plant growth regulators in forage seed production[J]. Pratacultural Science, 2017, 34(6):1238-1246.
[8] 宋吉轩. 干旱胁迫下植物生长调节剂对羊草生长及生理特性的影响与转录组分析[D]. 重庆:西南大学, 2017. [9] 位劼. 植物生长调节剂对油松种子萌发的影响[J]. 防护林科技, 2018(2):33-34. Wei J. Inffluence of plant growth regulator on seed germination of Pinus tabulaeformis[J]. Protection Forest Science and Technology, 2018(2):33-34.
[10] 樊海潮, 顾万荣, 尉菊萍, 王悦力, 孟瑶, 张立国, 李晶, 魏湜. 植物生长调节剂增强玉米抗倒伏能力的机制[J]. 江苏农业学报, 2017, 33(2):253-262. Fan HC, Gu WR, Wei JP, Wang YL, Meng Y, Zhang LG, Li J, Wei S. Mechanism of lodging resistance of maize improved by plant growth regulator[J]. Jiangsu Journal of Agricultural Sciences, 2017, 33(2):253-262.
[11] 唐中杰, 房卫平, 孙玉堂. 不同植物生长调节剂在棉花上的应用效果研究[J]. 中国农学通报, 2006(2):179-181. Tang ZJ, Fang WP, Sun YT. Studies on the effect of different kinds of plant growth regulator on cotton[J]. Chinese Agricultural Science Bulletin, 2006(2):179-181.
[12] 宁倩, 吴金水, 李宝珍, 吴蔓莉. 水稻苗期生长特性对不同浓度IAA的响应[J]. 农业现代化研究, 2013, 34(2):235-238. Ning Q, Wu JS, Li BZ, Wu ML. Response of growth cha-racteristics to different IAA concentrations in rice seedling[J]. Research of Agricultural Modernization, 2013, 34(2):235-238.
[13] 刘娟, 马小乐, 尚勋武, 王化俊. 外源IAA对小麦‘西旱2号’幼苗水分胁迫和NaCl胁迫的缓解响应[J].甘肃农业大学学报, 2009, 44(2):47-51. Liu J, Ma XL, Sang XW, Wang HJ. Regulation of exogenous auxin IAA on drought and salt stress during seedling stage of spring wheat (cv. Xihan No.2)[J]. Journal of Gansu Agricultural University, 2009, 44(2):47-51.
[14] Vicente O, Boscaiu M, Naranjo MÁ, Estrelles E, Bellés JM, Soriano P. Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae)[J]. J Arid Environ, 2004, 58(4):463-481.
[15] 张晓晓, 殷小琳, 李红丽, 苏丹, 贾淑友, 董智. NaCl胁迫对不同白榆品系生物量及光合作用的影响[J].生态学报, 2017, 37(21):7258-7265. Zhang XX, Yin XL, Li HL, Su D, Jia SY, Dong Z. Effect of salt stress on the biomass and photosynthetic characteristics of Ulmus pumila L. strains[J]. Ecological Journal, 2017, 37(21):7258-7265.
[16] 宗宁, 段呈, 耿守保, 柴曦, 石培礼, 何永涛. 增温施氮对高寒草甸生产力及生物量分配的影响[J]. 应用生态学报, 2018, 29(1):59-67. Zong N, Duan C, Geng SB, Chai X, Shi PL, He YT. Effects of warming and nitrogen addition on community production and biomass allocation in an alpine meadow[J]. Chinese Journal of Applied Ecology, 2018, 29(1):59-67.
[17] 李甜江, 李根前, 贺斌, 徐德兵. 克隆植物中国沙棘生长对外源植物激素的响应[J]. 生态学报, 2010, 30(3):659-667. Li TJ, Li GQ, He B, Xu DB. The growth of clonal plant Hippophae rhamnoides L. ssp. sinensis in response to exogenous plant hormones[J]. Ecological Journal, 2010, 30(3):659-667.
[18] Goto N, Miyazaki H, Nakamura NN, Terai H, Ishida N, Mitamura O. Relationships between electron transport rates determined by pulse amplitude modulated (PAM) chlorophyll fluorescence and photosynthetic rates by traditional and common methods in natural freshwater phytoplankton[J]. Fund Appl Limnol, 2008, 172(2):121-134.
[19] 许大全. 植物光胁迫研究中的几个问题[J]. 植物生理学通讯, 2003, 39(5):493-495. Xu DQ. Several problems in the research of plant light stress[J]. Plant Physiology Communications, 2003, 39(5):493-495.
[20] 王明. 外源植物生长调节剂提高番茄幼苗弱光逆境适应性的生理效应[D]. 北京:中国农业科学院, 2008. [21] 许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992(4):237-243. Xu DQ, Zhang YZ, Zhang RX. Photoinhibition of photosynthesis in plants[J]. Plant Physiology Communications, 1992(4):237-243.
[22] 胡留杰, 彭予咸, 徐泽, 邓敏,谢堃,皮利. 植物生长调节剂对茶新梢叶绿素荧光参数日变化的影响[J].西南农业学报, 2013, 26(4):1459-1462. Hu LJ, Peng YX, Xu Z, Deng M, Xie K, Pi L. Effects of plant growth regulators on daily change of chlorophyll fluorescence of tea shoots[J]. Southwest China Journal of Agricultural Sciences, 2013, 26(4):1459-1462.
[23] 孙华, 孙波, 张桃林. 中亚热带典型生态系统及其主要植物光合生理生态特性研究[J]. 中国生态农业学报, 2002(2):11-13. Sun H, Sun B, Zhang TL. The photosynthesis of the typical ecosystems and their primary plants in the middle subtropical zone[J]. Chinese Journal of Ecological Agriculture, 2002(2):11-13.
[24] 郑国琦, 许兴, 徐兆桢, 刘振荣. 盐胁迫对枸杞光合作用的气孔与非气孔限制[J]. 西北植物学报, 2002(6):75-79. Zheng GQ, Xu X, Xu ZZ, Liu ZR. The effect of salt stress on the stomatal and norrstomatal limitation of photosynthesis of Lycium barbarum[J]. Acta Botanica Boreali-Occidentalia Sinica, 2002(6):75-79.
[25] 徐萍, 李进, 吕海英, 李永洁,张侠. 干旱胁迫下水杨酸对银沙槐子叶表皮气孔开度的影响[J]. 植物生理学报, 2014, 50(4):510-518. Xu P, Li J, Lu HY, Li YJ, Zhang X. Effect of salicylic acid on stomata aperture of epidermis in Ammodendron argenteum cotyled under drought stress[J]. Plant Physiology Journal, 2014, 50(4):510-518.
[26] 郭连金, 杜佳朋, 吴艳萍, 肖志鹏, 廖婷, 邵秀娟. 香果树实生苗的光合特性及其与环境因子的关系[J].应用生态学报, 2017, 28(5):1473-1481. Guo LJ, Du JP, Wu YP, Xiao ZP, Liao T, Shao XJ. Photosynthetic characteristics of Emmenopterys henryi seedlings and their relationships with environmental factors[J]. Chinese Journal of Applied Ecology, 2017, 28(5):1473-1481.
[27] 曲天明. DCPTA对寒地玉米叶片光合特性及根系生长的影响[D]. 哈尔滨:东北农业大学, 2014.
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