Genetic and morphological variation patterns of Macromitrium gymnostomum and their geographical backgrounds
-
摘要: 缺齿蓑藓（Macromitrium gymnostomum Sull.&Lesq.）广布于我国东南部，形态变异较大，与近缘种关系模糊。为了正确鉴定缺齿蓑藓形态变异范围，以及形态、遗传和地理因素三者的关系，对缺齿蓑藓11个地理居群106个样本配子体的13个形态性状进行了测定。结果显示，根据形态数据可将106份样本聚类成8个形态组。用13对ISSR引物获得了150个位点，其中148个为多态位点，多态位点百分率为98.67%；Nei’s基因多样性在居群内占62.22%，在居群间占37.78%；居群间基因流（Nm）为0.8235，遗传分化指数（Gst）为0.3778。11个地理居群的遗传距离在0.0873~0.2363之间，平均为0.1508。基于148个多态位点可将106份样本聚成8个遗传组。缺齿蓑藓的形态变异有一定的遗传背景（r=0.159，n=106，P < 0.2），地理因素对形态（r=0.309，n=106，P < 0.01）和遗传（r=0.251，n=106，P < 0.01）分化产生了极显著影响。Abstract: Macromitrium gymnostomum Sull. & Lesq. is widely distributed in southeastern China, with high morphological variation and confusion with allied species. To correctly define the morphological variation range of M. gymnostomum and the relationship among morphological patterns, genetics, and geographical factors, 13 morphological traits based on 106 samples from 11 populations of M. gymnostomum were determined. The 106 samples were preliminarily clustered into eight groups based on the 13 morphological traits. Using 13 pairs of ISSR primers, 150 ISSR bands were amplified, of which 148 (98.67%) were polymorphic. The intra-population genetic diversity accounted for 62.22%, whereas the inter-population value accounted for 37.78%. Gene flow existed among populations, with a Nm index of 0.8235 and a Gst of 0.3778. The genetic distance among the 11 geographical populations varied from 0.0873 to 0.2363, with an average of 0.1508. Based on information from the 148 loci, the 106 samples were clustered into eight groups. The morphological variations of M. gymnostomum were genetically controlled to an extent (r=0.159, n=106, P < 0.2). Distinctive geographical influences on morphological (r=0.309, n=106, P < 0.01) and genetic differentiation (r=0.251, n=106, P < 0.01) were also found.
-
-
[1] 吴鹏程, 贾渝. 中国苔藓志:第5卷[M]. 北京:科学出版社, 2011:86-99. Wu PC, Jia Y. Flora Bryophytorum Sinicorum:Vol. 5[M]. Beijing:Science Press, 2011:86-99.
[2] Potier de la Varde R. Recoltes bryologiques en Afrique Equatoriale Francaise[J]. Ann Cryptog Exot, 1932, 5:53-67.
[3] Brotherus VF. Musci novi Japonici[J]. Öfvers Finska Vetensk.-Soc Förh, 1919-1920, 62A(9):1-55.
[4] Mitten W. On some species of Musci and Hepaticae, additional to the floras of Japan and the coast of China[J]. J Proc Linn Soc Bot, 1864, 8:148-162.
[5] Guo SL, Cao T, Tan BC, Song GY. Taxonomic notes onAsian species of Orthotrichaceae (Bryopsida):Macromitrium with gymnostomous capsules[J]. Gard Bull Singapore, 2007, 58(2):155-178.
[6] 李丹丹, 郭水良, 于晶, 李莎, 曹同. 基于4个叶绿体基因识别蓑藓属(Macromitrium)植物的可行性研究[J]. 植物科学学报, 2013, 31(1):23-33. Li DD, Guo SL, Yu J, Li S, Cao T. Feasibility study on the Identification of genus Macromitrium based on four chloroplast genes[J]. Plant Science Journal, 2013, 31(1):23-33.
[7] Yu J, Guo SL, Ma YH, Cao T. Taxonomic and morphometric comments on Macromitrium tosae Besch. (Orthotrichaceae), with its four new synonyms[J]. Bryologist, 2012, 115(3):388-401.
[8] 汪琛颖, 赵建成. 狭边大叶藓(Rhodobryum ontariense)遗传多样性的ISSR分析[J]. 贵州师范大学学报:自然科学版, 2010, 28(4):33-40. Wang CY, Zhao JC. Genetic diversity in a medicinal plant species:Rhodobryum ontariense(Bryaceae), detected by ISSR[J]. Journal of Guizhou Normal University:Natural Sciences, 2010, 28(4):33-40.
[9] 郭水良, 郑园园, 娄玉霞, 沈蕾. 基于RAPD数据探讨中华蓑藓(Macromitrium cavaleriei Card. & Thér.)形态变异的遗传基础[J]. 植物科学学报, 2011, 29(3):312-318. Guo SL, Zhen YY, Lou YX, Shen L. Genetic background of morphological variation in Macromitrium cavaleriei Card. & Thér. based on RAPD[J]. Plant Science Journal, 2011, 29(3):312-318.
[10] Spagnuolo V, Terracciano S, Cobianchi RC. Taxonomy of the Hypnum cupressiforme complex in Italy based on ITS and trnL sequences and ISSR markers[J]. Trans British Bryolog Society, 2013, 30(4):283-289.
[11] Shaw AJ, Andrus RE, Shaw B. Sphagnum beringiense sp. nov. (Bryophyta) from Arctic Alaska, based on morphological and molecular data[J]. Syst Bot, 2008, 33(3):469-477.
[12] 刘丽, 朱永青, 王幼芳. 鼠尾藓不同居群间形态及RAPD分析[J]. 云南植物研究, 2006, 28(6):570-574. Liu L, Zhu YQ, Wang YF. Morphological and RAPD analyses of different Myuroclada maxiowiczii (Brachy-theciaceae) populations[J]. Acta Botanica Yunnanica, 2006, 28(6):570-574.
[13] 李朝阳, 李菁, 田向荣, 姜业芳, 陈军. 梵净山尖叶拟船叶藓的遗传多样性分析[J]. 云南植物研究, 2008, 30(2):168-172. Li ZY, Li J, Tian XR, Jiang YF, Chen J. Analysis on genetic diversity of Dolichomitriopsis diversiformis (Lembophyllaceae) in Fanjing Mountain[J]. Acta Botanica Yunnanica, 2008, 30(2):168-172.
[14] 李倩影, 曹同, 于晶, 俞鹰浩. 不同种群狭叶小羽藓(Haplocladium angustifolium)重金属含量及遗传多样性[J]. 上海师范大学学报:自然科学版, 2010, 39(2):194-199. Li QY, Cao T, Yu J, Yu YH. Genetic diversity and heavy metal contents of different populations of the moss Haplocladium angustifolium[J]. Journal of Shanghai Normal University:Natural Sciences, 2010, 39(2):194-199.
[15] 张晗, 高永超, 沙伟, 李汝玉, 戴双. 紫萼藓科植物DNA提取及遗传多样性的分析[J]. 山东农业科学, 2006(1):7-9. Zhang H, Gao YC, Sha W, Li RY, Dai S. Study of DNA extraction and genetic diversity in Grimmiaceae[J]. Shandong Agricultural Sciences, 2006(1):7-9.
[16] 戴智慧, 龙骏, 俞雷民, 倪穗. 宁波地区野生换锦花遗传多样性的ISSR分析[J]. 核农学报, 2016, 30(10):1898-1905. Dai ZH, Long J, Yu LM, Ni S. Genetic diversity of wide Lycorissprengeri populations from different habitats of Ningbo based on ISSR analysis[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(10):1898-1905.
[17] 李倩影. 小羽藓属(Haplocladium)植物遗传多样性及其与环境因子关系分析[D]. 上海:上海师范大学, 2010:41-42. Li QY. Genetic biodiversity of moss genus Haplocladium and its relationship with the environmental factors[D]. Shanghai:Shanghai Normal University, 2010:41-42.
[18] 孙鹏雁. 中国曲柄藓属(Campylopus)植物的形态与系统学研究[D]. 上海:上海师范大学, 2015:66. Sun PY. A morphological and phylogenetic study on Campylopus of China[D]. Shanghai:Shanghai Normal University, 2015:66.
[19] 魏青永, 郭水良, 曹同, 于晶. 基于ISSR数据探讨卷叶凤尾藓(Fissidens dubius P. Beauv.)遗传多样性[J]. 植物科学学报, 2016, 34(2):238-245. Wei QY, Guo SL, Cao T, Yu J. Genetic diversity of Fissidens dubius P. Beauv. based on ISSR data[J]. Plant Science Journal, 2016, 34(2):238-245.
[20] 王莹莹. 浙江千岛湖生境片断化对苔藓植物物种及遗传多样性的影响[D]. 上海:华东师范大学, 2011:36. Wang YY. Effects of habitat fragmentation on bryophytes richness and genetic diversity in Thousand-Island Lake region, Zhejiang[D]. Shanghai:East China Normal University, 2011:36. [21] 石蕾. R语言在藓类形态与遗传变异研究中的应用——以大灰藓为例[D]. 上海:上海师范大学, 2015:47. Shi L. Application of R in studies of morphological and genetic variation of plant populations-A case of Hypnum plumaeforme Wilson[D]. Shanghai:Shanghai Normal University, 2015:47. -
期刊类型引用(17)
1. 徐磊，胥晓，刘沁松. 外源水杨酸对盐胁迫下珙桐幼苗抗氧化系统和基因表达的影响. 植物研究. 2023(04): 572-581 . 
百度学术
2. 张爱慧，冷欣兰，袁颖辉，任慧羚，陈雪琼，朱士农. 5-ALA对NaCl胁迫下丝瓜幼苗生长及生理特性的影响. 江苏农业科学. 2023(13): 137-141 . 百度学术
3. 朱普生，刘慧英，曹泽，丛蕴郸. 番茄GAPDH基因家族的鉴定及其在GSNO调控番茄盐胁迫中的响应. 分子植物育种. 2023(23): 7682-7688 . 百度学术
4. 张雪蒙，亢超，滕元旭，陈静怡，崔辉梅. 外源硫化氢和水杨酸对盐胁迫下加工番茄幼苗生长与生理特性的影响. 西北植物学报. 2022(02): 255-262 . 百度学术
5. 吴莺，张淑英，陈明媛，王梦柯. SNP对盐胁迫下棉花幼苗光合抑制及氧化损伤的缓解效应. 植物生理学报. 2022(04): 757-766 . 百度学术
6. 左月桃，董玲，任晓松，刘赵月，左师宇，李晶. 外源褪黑素对盐碱胁迫下小黑麦种子萌发幼苗生长、抗氧化能力的影响. 麦类作物学报. 2022(01): 90-99 . 百度学术
7. 赵野，刘威，王贺，吴华鑫，肖雅楠，闫永庆. 外源CaCl_2对盐胁迫下西伯利亚白刺活性氧代谢的影响. 植物生理学报. 2021(05): 1105-1112 . 百度学术
8. 程园，李灿婴，侯佳宝，李雪，王晓涵，葛永红. 采后硝普钠处理对南果梨果实贮藏品质和细胞壁降解酶的影响. 食品科学. 2020(01): 252-257 . 百度学术
9. 耿贵，李任任，吕春华，於丽华，王宇光. 外源调节物质对盐胁迫下植物生长调控研究进展. 中国农学通报. 2020(24): 85-90 . 百度学术
10. 刘赵月，李蕊彤，李晶，顾万荣，左师宇，任晓松，左月桃，魏湜. 盐碱胁迫下京尼平苷对玉米种子萌发及根系AsA-GSH循环的影响. 江苏农业学报. 2020(04): 842-850 . 百度学术
11. 赵宝泉，邢锦城，王静，朱小梅，刘冲，洪立洲. 水杨酸对盐胁迫下杭白菊幼苗生长和生理特性的影响. 吉林农业大学学报. 2020(04): 370-379 . 百度学术
12. 普凌，赵鑫，王艇越，侯浩南，张毅. 等渗盐胁迫对番茄幼苗生长和生理特性的影响. 陕西农业科学. 2019(05): 35-38 . 百度学术
13. 蒋景龙，沈季雪，李丽. 外源H_2O_2对盐胁迫下黄瓜幼苗氧化胁迫及抗氧化系统的影响. 西北农业学报. 2019(06): 998-1007 . 百度学术
14. 孙德智，杨恒山，张庆国，范富，苏雅乐其其格，彭靖，韩晓日. 外源一氧化氮供体硝普钠对番茄幼苗盐胁迫伤害的缓解作用. 浙江农业学报. 2019(08): 1286-1294 . 百度学术
15. 李翀，王杰，贾赵辉，程雪飞，彭孝楠，陈颖，张金池. 南林‘895’杂交杨组培苗对NaCl胁迫的生理响应. 安徽农业大学学报. 2019(06): 961-967 . 百度学术
16. 李海萍. 盐胁迫及外源物质对植物抗盐性影响的研究进展. 青海农技推广. 2018(04): 48-50 . 百度学术
17. 董亚茹，赵东晓，杜建勋，孙景诗，陈传杰，王照红. 外源NO对NaCl胁迫下桑树种子萌发及幼苗生理生化特性的影响. 蚕业科学. 2018(06): 821-827 . 百度学术
其他类型引用(16)
计量
- 文章访问数: 702
- HTML全文浏览量: 2
- PDF下载量: 677
- 被引次数: 33
下载:
