中国蜘蛛抱蛋属植物DNA条形码研究

吕定豪; 刘安莉; 徐文芬; 何顺志

doi:10.11913/PSJ.2095-0837.2018.60784

中国蜘蛛抱蛋属植物DNA条形码研究

1. 遵义市第四人民医院, 贵州遵义 563004;
2. 贵阳中医学院第二附属医院, 贵阳 550002;
3. 贵阳中医学院, 贵阳 550001

基金项目:

国家自然科学基金项目（81660640）；贵州省科技计划项目（黔科合LH[2016]7132号）；贵州省教育厅国内一流学科建设项目（GNYL[2017]008）。

详细信息

作者简介:
吕定豪(1990-),男,硕士研究生,研究方向为中药及民族药质量与鉴定(E-mail:365173732@qq.com)。

通讯作者:
何顺志,E-mail:hesz8899@126.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2018-06-07
- 网络出版日期: 2022-10-31
- 发布日期: 2018-12-27

Study on the genus Aspidistra based on DNA barcoding

1. The Fourth People's Hospital of Zunyi, Zunyi 563004, China;
2. The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;
3. Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (8160640), Science and Technology Planning Project of Guizhou Province of China (LH[2016]7132), and First-class Discipline Construction Project of Guizhou Provincial Education Department (GNYL[2017]008).

摘要

摘要: 利用植物DNA条形码候选序列matK、psbA-trnH、psbK-psbI和rbcL对蜘蛛抱蛋属（Aspidistra）植物的19种104批样品进行扩增和测序，并采用相似性搜索算法（BLAST）对各序列的鉴定效率进行评价，得出蜘蛛抱蛋属物种鉴定的最佳序列。结果显示，psbK-psbI的物种鉴定成功率为88.7%，在单一序列中成功率最高。通过多序列组合鉴定效率的比较，发现组合序列的鉴定成功率明显高于单一序列，其中matK+（psbK-psbI）组合的鉴定成功率高达100%，基于该序列组合构建蜘蛛抱蛋属植物的系统发育树，结果显示同一物种的样品聚集度较好，多表现为单系。研究结果表明matK+（psbK-psbI）序列组合可作为蜘蛛抱蛋植物种鉴定的最佳条形码序列。
- 蜘蛛抱蛋属 /
- DNA条形码 /
- 相似性搜索算法 /
- 鉴定
Abstract: In this study, plant DNA barcode (matK, psbA-trnH, psbK-psbI, and rbcL) candidate sequences were used for the PCR amplification and sequencing of 104 samples from 19 Aspidistra species. The amplification success rate, sequencing success rate, and intra- and inter-species genetic variation of each barcode sequence were investigated, with BLAST searching adopted to evaluate the identification efficiency of each sequence. Results showed that the identification success rate of psbK-psbI was 88.7%, which was the highest for a single sequence. The identification efficiency of multiple combinational sequences was also compared and found to be significantly higher than that of the single sequences, with the success rate of matK+(psbK-psbI) reaching 100%. In addition, the neighbor-joining tree constructed based on the matK+(psbK-psbI) combinational sequence showed that samples of the same species had better aggregation and were mostly monophyletic. Therefore, matK+(psbK-psbI) appears to be the best barcode sequence for interspecific identification of Aspidistra.
- Aspidistra /
- DNA barcoding /
- BLAST /
- Identification

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