茎杆的第一节间离体再生空心菜

郝园园; 舒黄英; 杨壮; 李雯; 苏晓; 周燕; 高崇伦; 朱国鹏; 成善汉; 汪志伟

doi:10.11913/PSJ.2095-0837.2019.10047

茎杆的第一节间离体再生空心菜

海南大学热带农林学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228

基金项目: 海南省研究生创新科研课题（Hys2017-87）；海南大学高层次人才启动基金（KYQD1656）

详细信息

作者简介:
郝园园(1992-),女,硕士研究生,研究方向为植物发育生物学(E-mail:627968306@qq.com)

通讯作者:
汪志伟.Email:zwwang22@163.com,wangzhiwei@hainu.edu.cn

中图分类号: Q943.1
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出版历程
- 收稿日期: 2018-09-29
- 修回日期: 2018-10-25
- 发布日期: 2019-02-27

In vitro plant regeneration from first stem internode explants of water spinach (Ipomoea aquatica)

Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

Funds: This work was supported by grants from the Innovative Research Projects for Postgraduates in Hainan Province (Hys2017-87) and Startup Funding from Hainan University (KYQD1656)

摘要

摘要: 以空心菜（Ipomoea aquatica Forsk.）品种‘白骨柳叶’为材料，通过筛选植物外植体和调整培养基激素配比等方法，首次建立了茎秆第一节间为外植体的空心菜离体再生体系。结果表明，在MS基本培养上添加0.05% 的植物组织培养抗菌剂PPM可获得大量空心菜无菌苗；植株子叶和下胚轴的切段均未诱导出不定芽，而茎秆第一节间为外植体能成功诱导出不定芽，诱导成功率为20%，最佳培养基配方为MS+1.0 mg/L 6-BA+0.1 mg/L IAA；不定芽诱导生根的最佳培养基配方为1/2 MS+0.1 mg/L NAA，生根率为100%。诱导成功后，将完整的再生苗移栽至基质土中，成活率可达100%。
- 空心菜 /
- 不定芽 /
- 第一节间 /
- 再生体系
Abstract: In this experiment, white bone willow leaf spinach (Ipomoea aquatica Forsk.) was used as research material. By screening explants and hormone ratios, an in vitro regeneration system of I. aquatica from first stem internode explants was established for the first time. Results showed that a large number of sterile I. aquatica seedlings could be obtained by adding 0.05% PPM to the basic culture of MS. Explants of cotyledons and hypocotyls did not induce adventitious buds; however, the first stem internode successfully induced adventitious buds. The optimal medium was MS+1.0 mg/L 6-BA+0.1 mg/L IAA, and the adventitious bud induction rate was 20%. The optimal medium for inducing adventitious bud roots was 1/2 MS + 0.1 mg/L NAA, with the rooting rate reaching 100%. The whole regenerated seedlings were transplanted into matrix soil, with a transplant survival rate of 100%. This study established a spinach regeneration system and laid the foundation for further research on transgenic I. aquatica.
- Ipomoea aquatica /
- Adventitious bud /
- First stem internode /
- Regeneration system

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