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摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究

李琰, 赵磊, 温鹏飞, 葛付华, 张兴

李琰, 赵磊, 温鹏飞, 葛付华, 张兴. 摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究[J]. 植物科学学报, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093
引用本文: 李琰, 赵磊, 温鹏飞, 葛付华, 张兴. 摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究[J]. 植物科学学报, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093
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Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093
Citation: Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. DOI: 10.11913/PSJ.2095-0837.2019.10093
shu
李琰, 赵磊, 温鹏飞, 葛付华, 张兴. 摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究[J]. 植物科学学报, 2019, 37(1): 93-100. CSTR: 32231.14.PSJ.2095-0837.2019.10093
引用本文: 李琰, 赵磊, 温鹏飞, 葛付华, 张兴. 摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究[J]. 植物科学学报, 2019, 37(1): 93-100. CSTR: 32231.14.PSJ.2095-0837.2019.10093
shu
Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. CSTR: 32231.14.PSJ.2095-0837.2019.10093
Citation: Li Yan, Zhao Lei, Wen Peng-Fei, Ge Fu-Hua, Zhang Xing. Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions[J]. Plant Science Journal, 2019, 37(1): 93-100. CSTR: 32231.14.PSJ.2095-0837.2019.10093
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摇瓶培养条件下雷公藤不定根生长及次生代谢产物含量的研究

  • 1.

    西北农林科技大学生命科学学院, 陕西杨凌 712100

  • 2. 陕西省生物农药工程技术研究中心, 陕西杨凌 712100

基金项目: 国家自然科学基金项目（31272110）；国家高技术研究发展计划（863）项目子课题（2011AA10A202）
详细信息
    作者简介:

    李琰(1971-),女,博士,副教授,主要从事经济植物细胞培养及次生代谢研究(E-mail:ly2659@163.com)

    通讯作者:

    李琰.Email:ly2659@163.com

  • 中图分类号: Q946.8

Study on the adventitious root culture of Tripterygium wilfordii under shaking culture conditions

  • 1.

    College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China

  • 2. Shaanxi Province Technology and Engineering Center of Biopesticide, Yangling, Shaanxi 712100, China

Funds: This work was supported by grants from the National Natural Science Foundation of China (31272110) and National High Techno-logy Research and Development Program of China (863 Program) (2011AA10A202)

摘要

    摘要
  • 摘要: 以雷公藤（Tripterygium wilfordii Hook. f.）不定根为材料，研究摇瓶悬浮培养条件下接种密度、装液比例、逐级放大、消泡剂、大孔吸附树脂种类及浓度对雷公藤不定根增长量、不定根及培养基中雷公藤内酯醇、雷公藤吉碱、雷公藤次碱含量的影响。结果显示，接种密度在15 g/L （FW）时较适合不定根的继代培养和次生代谢产物的积累。250 mL摇瓶中装入100 mL培养基，即装液量为2/5时，培养基利用率最高。随着摇瓶体积的逐渐放大，不定根增长量和3种次生代谢产物含量略有下降，5 L摇瓶中不定根增长量为对照的91.6%，内酯醇、吉碱和次碱的含量分别为对照的91.8%、91.7%和96.9%。6种大孔吸附树脂中，XAD-7处理对不定根的生长有明显促进作用，培养结束时，3种次生代谢产物产量显著提高，当XAD-7浓度为0.5 g/瓶时不定根增长量为对照的1.2倍，内酯醇、吉碱和次碱产量最高，分别为对照的2.9、2.4和2.2倍。培养基中添加消泡剂后不定根增长量、3种次生代谢产物总产量均不同程度下降，其中，LX-603处理后，虽然不定根增长量为对照的85%，内酯醇、吉碱和次碱产量分别为对照的78%、64%和87%，但明显抑制了培养过程中泡沫的产生。研究结果表明筛选的摇瓶逐级放大培养雷公藤不定根的方法效果较好，可为雷公藤不定根生物反应器放大培养奠定基础。
    Abstract: Tripterygium wilfordii Hook. f., adventitious roots were used in vitro to study the effects of inoculation density, liquid medium volume per flask, step-by-step amplification, defoaming agent, macroporous adsorption (MA) resin type, and concentrations of triptolide, wilforgine, and wilforine in roots and medium on adventitious root growth after shake-flask culture. Results showed that an inoculation density of 15 g/L was suitable for the subculture of adventitious roots and accumulation of secondary metabolite. The medium utilization rate was the highest in a 250-mL flask with 100 mL of liquid medium. Adventitious root growth declined slightly with the increase in flask volume. In a 5-L bottle, the adventitious root growth and triptolide, wilforgine, and wilforine concentrations were 91.6%, 91.8%, 91.7%, and 96.9%, respectively, compared to the control， XAD-7 resin markedly enhanced adventitious root growth and secondary metabolite content among the six kinds of MA resin. The adventitious root growth and triptolide, wilforgine, and wilforine content increased 1.2-, 2.9-, 2.4-, and 2.2-fold, respectively, compared to the control when XAD-7 resin was at 0.5 g/bottle. Adventitious root growth and secondary metabolite content all decreased at differing degrees when the defoaming agent was added to the medium. Adventitious root growth and triptolide, wilforgine, and wilforine contents were 85%, 78%, 64%, and 87%, respectively, compared to the control when LX-603 was added to the medium; however, foam was markedly inhibited during the culturing process. This study demonstrated that step-by-step amplification was a feasible approach for adventitious root culture. Furthermore, this research provides the basis for the scale-up culture of T. wilfordii adventitious roots using a bioreactor.
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  • 收稿日期:  2018-07-08
  • 修回日期:  2018-08-31
  • 发布日期:  2019-02-27

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